Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS12340 and DZA65_RS12345 are separated by 141 nucleotides DZA65_RS12345 and DZA65_RS12350 are separated by 12 nucleotides DZA65_RS12350 and DZA65_RS12355 overlap by 4 nucleotides DZA65_RS12355 and DZA65_RS12360 are separated by 1 nucleotides
DZA65_RS12340: DZA65_RS12340 - amidohydrolase, at 2,635,029 to 2,636,231
_RS12340
DZA65_RS12345: DZA65_RS12345 - SDR family oxidoreductase, at 2,636,373 to 2,637,152
_RS12345
DZA65_RS12350: DZA65_RS12350 - ABC transporter ATP-binding protein, at 2,637,165 to 2,638,862
_RS12350
DZA65_RS12355: DZA65_RS12355 - ABC transporter permease, at 2,638,859 to 2,639,728
_RS12355
DZA65_RS12360: DZA65_RS12360 - ABC transporter permease, at 2,639,730 to 2,640,716
_RS12360
Position (kb)
2637
2638
2639 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2636.168 kb on + strand at 2636.199 kb on + strand at 2636.200 kb on - strand at 2636.200 kb on - strand at 2636.200 kb on - strand at 2636.200 kb on - strand at 2636.200 kb on - strand at 2636.221 kb on + strand at 2636.221 kb on + strand at 2636.221 kb on + strand at 2636.221 kb on + strand at 2636.221 kb on + strand at 2636.222 kb on - strand at 2636.334 kb on + strand at 2636.536 kb on + strand, within DZA65_RS12345 at 2636.537 kb on - strand, within DZA65_RS12345 at 2636.612 kb on + strand, within DZA65_RS12345 at 2636.613 kb on - strand, within DZA65_RS12345 at 2636.613 kb on - strand, within DZA65_RS12345 at 2636.638 kb on - strand at 2636.641 kb on - strand, within DZA65_RS12345 at 2636.954 kb on + strand, within DZA65_RS12345 at 2636.954 kb on + strand, within DZA65_RS12345 at 2636.954 kb on + strand, within DZA65_RS12345 at 2636.954 kb on + strand, within DZA65_RS12345 at 2636.955 kb on - strand, within DZA65_RS12345 at 2637.117 kb on + strand at 2637.117 kb on + strand at 2637.117 kb on + strand at 2637.118 kb on - strand at 2637.118 kb on - strand at 2637.166 kb on + strand at 2637.166 kb on + strand at 2637.166 kb on + strand at 2637.167 kb on - strand at 2637.167 kb on - strand at 2637.167 kb on - strand at 2637.242 kb on + strand at 2637.242 kb on + strand at 2637.242 kb on + strand at 2637.242 kb on + strand at 2637.242 kb on + strand at 2637.243 kb on - strand at 2637.244 kb on + strand at 2637.244 kb on + strand at 2637.244 kb on + strand at 2637.245 kb on - strand at 2637.245 kb on - strand at 2637.334 kb on + strand at 2637.334 kb on + strand at 2637.334 kb on + strand at 2637.394 kb on - strand, within DZA65_RS12350 at 2637.630 kb on + strand, within DZA65_RS12350 at 2637.630 kb on + strand, within DZA65_RS12350 at 2637.630 kb on + strand, within DZA65_RS12350 at 2637.630 kb on + strand, within DZA65_RS12350 at 2637.630 kb on + strand, within DZA65_RS12350 at 2637.630 kb on + strand, within DZA65_RS12350 at 2637.630 kb on + strand, within DZA65_RS12350 at 2637.631 kb on - strand, within DZA65_RS12350 at 2637.631 kb on - strand, within DZA65_RS12350 at 2637.631 kb on - strand, within DZA65_RS12350 at 2637.631 kb on - strand, within DZA65_RS12350 at 2637.631 kb on - strand, within DZA65_RS12350 at 2637.755 kb on + strand, within DZA65_RS12350 at 2637.755 kb on + strand, within DZA65_RS12350 at 2637.756 kb on - strand, within DZA65_RS12350 at 2637.759 kb on - strand, within DZA65_RS12350 at 2637.759 kb on - strand, within DZA65_RS12350 at 2637.996 kb on - strand, within DZA65_RS12350 at 2637.996 kb on - strand, within DZA65_RS12350 at 2638.127 kb on + strand, within DZA65_RS12350 at 2638.129 kb on + strand, within DZA65_RS12350 at 2638.129 kb on + strand, within DZA65_RS12350 at 2638.129 kb on + strand, within DZA65_RS12350 at 2638.129 kb on + strand, within DZA65_RS12350 at 2638.130 kb on - strand, within DZA65_RS12350 at 2638.833 kb on + strand at 2638.862 kb on + strand at 2638.862 kb on + strand at 2638.862 kb on + strand at 2638.862 kb on + strand at 2638.862 kb on + strand at 2638.863 kb on - strand at 2638.863 kb on - strand at 2638.863 kb on - strand at 2638.863 kb on - strand at 2638.863 kb on - strand at 2638.863 kb on - strand at 2638.863 kb on - strand at 2638.863 kb on - strand at 2639.212 kb on + strand, within DZA65_RS12355 at 2639.333 kb on - strand, within DZA65_RS12355 at 2639.333 kb on - strand, within DZA65_RS12355 at 2639.657 kb on - strand at 2639.783 kb on + strand at 2639.783 kb on + strand at 2639.784 kb on - strand at 2639.854 kb on - strand, within DZA65_RS12360
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,636,168 + -0.8 2,636,199 + +0.8 2,636,200 - +0.1 2,636,200 - +0.9 2,636,200 - +0.5 2,636,200 - -0.3 2,636,200 - +0.7 2,636,221 + +0.7 2,636,221 + +1.0 2,636,221 + -0.5 2,636,221 + -0.8 2,636,221 + -0.0 2,636,222 - -3.4 2,636,334 + +0.9 2,636,536 + DZA65_RS12345 0.21 +0.8 2,636,537 - DZA65_RS12345 0.21 +0.7 2,636,612 + DZA65_RS12345 0.31 -0.5 2,636,613 - DZA65_RS12345 0.31 -1.0 2,636,613 - DZA65_RS12345 0.31 -0.8 2,636,638 - +0.2 2,636,641 - DZA65_RS12345 0.34 -0.5 2,636,954 + DZA65_RS12345 0.74 -0.1 2,636,954 + DZA65_RS12345 0.74 +1.1 2,636,954 + DZA65_RS12345 0.74 -0.6 2,636,954 + DZA65_RS12345 0.74 +0.2 2,636,955 - DZA65_RS12345 0.75 -2.1 2,637,117 + +1.5 2,637,117 + +1.5 2,637,117 + -0.2 2,637,118 - +0.6 2,637,118 - +1.4 2,637,166 + -1.2 2,637,166 + -0.8 2,637,166 + +1.0 2,637,167 - +0.6 2,637,167 - +1.8 2,637,167 - -0.3 2,637,242 + -0.9 2,637,242 + +1.5 2,637,242 + -0.3 2,637,242 + +0.2 2,637,242 + +1.6 2,637,243 - +0.2 2,637,244 + -0.8 2,637,244 + +0.9 2,637,244 + -0.0 2,637,245 - +0.3 2,637,245 - -1.0 2,637,334 + -0.0 2,637,334 + -0.3 2,637,334 + +0.6 2,637,394 - DZA65_RS12350 0.13 +0.5 2,637,630 + DZA65_RS12350 0.27 +1.1 2,637,630 + DZA65_RS12350 0.27 -0.2 2,637,630 + DZA65_RS12350 0.27 +0.7 2,637,630 + DZA65_RS12350 0.27 -0.1 2,637,630 + DZA65_RS12350 0.27 -1.6 2,637,630 + DZA65_RS12350 0.27 -0.0 2,637,630 + DZA65_RS12350 0.27 +0.9 2,637,631 - DZA65_RS12350 0.27 -1.2 2,637,631 - DZA65_RS12350 0.27 -0.3 2,637,631 - DZA65_RS12350 0.27 +0.2 2,637,631 - DZA65_RS12350 0.27 -0.4 2,637,631 - DZA65_RS12350 0.27 +0.7 2,637,755 + DZA65_RS12350 0.35 -0.1 2,637,755 + DZA65_RS12350 0.35 +0.8 2,637,756 - DZA65_RS12350 0.35 +0.6 2,637,759 - DZA65_RS12350 0.35 +0.6 2,637,759 - DZA65_RS12350 0.35 +0.3 2,637,996 - DZA65_RS12350 0.49 +0.3 2,637,996 - DZA65_RS12350 0.49 +0.4 2,638,127 + DZA65_RS12350 0.57 -0.2 2,638,129 + DZA65_RS12350 0.57 -0.5 2,638,129 + DZA65_RS12350 0.57 +0.2 2,638,129 + DZA65_RS12350 0.57 -0.4 2,638,129 + DZA65_RS12350 0.57 -1.1 2,638,130 - DZA65_RS12350 0.57 +1.8 2,638,833 + -0.9 2,638,862 + -0.1 2,638,862 + -2.1 2,638,862 + +1.0 2,638,862 + +0.3 2,638,862 + -2.6 2,638,863 - -0.4 2,638,863 - -1.6 2,638,863 - +0.2 2,638,863 - -0.1 2,638,863 - +0.2 2,638,863 - -0.1 2,638,863 - -1.2 2,638,863 - -1.0 2,639,212 + DZA65_RS12355 0.41 -0.4 2,639,333 - DZA65_RS12355 0.54 +0.1 2,639,333 - DZA65_RS12355 0.54 +0.3 2,639,657 - -0.7 2,639,783 + -0.9 2,639,783 + +0.5 2,639,784 - -0.3 2,639,854 - DZA65_RS12360 0.13 +0.2
Or see this region's nucleotide sequence