Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10045 and DZA65_RS10050 are separated by 14 nucleotides DZA65_RS10050 and DZA65_RS10055 overlap by 4 nucleotides
DZA65_RS10045: DZA65_RS10045 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 2,125,937 to 2,127,004
_RS10045
DZA65_RS10050: DZA65_RS10050 - histidinol-phosphate transaminase, at 2,127,019 to 2,128,089
_RS10050
DZA65_RS10055: DZA65_RS10055 - histidinol dehydrogenase, at 2,128,086 to 2,129,396
_RS10055
Position (kb)
2127
2128
2129 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2126.163 kb on + strand, within DZA65_RS10045 at 2126.163 kb on + strand, within DZA65_RS10045 at 2126.163 kb on + strand, within DZA65_RS10045 at 2126.164 kb on - strand, within DZA65_RS10045 at 2126.331 kb on + strand, within DZA65_RS10045 at 2126.332 kb on - strand, within DZA65_RS10045 at 2126.545 kb on + strand, within DZA65_RS10045 at 2126.545 kb on + strand, within DZA65_RS10045 at 2126.546 kb on - strand, within DZA65_RS10045 at 2126.628 kb on + strand, within DZA65_RS10045 at 2126.628 kb on + strand, within DZA65_RS10045 at 2126.628 kb on + strand, within DZA65_RS10045 at 2126.629 kb on - strand, within DZA65_RS10045 at 2126.992 kb on - strand at 2126.992 kb on - strand at 2126.992 kb on - strand at 2127.020 kb on + strand at 2127.020 kb on + strand at 2127.021 kb on - strand at 2127.021 kb on - strand at 2127.221 kb on + strand, within DZA65_RS10050 at 2127.222 kb on - strand, within DZA65_RS10050 at 2127.223 kb on + strand, within DZA65_RS10050 at 2127.232 kb on + strand at 2127.232 kb on + strand, within DZA65_RS10050 at 2127.539 kb on + strand, within DZA65_RS10050 at 2127.540 kb on - strand, within DZA65_RS10050 at 2127.540 kb on - strand, within DZA65_RS10050 at 2127.647 kb on + strand, within DZA65_RS10050 at 2127.647 kb on + strand, within DZA65_RS10050 at 2127.647 kb on + strand, within DZA65_RS10050 at 2127.648 kb on - strand, within DZA65_RS10050 at 2127.762 kb on - strand, within DZA65_RS10050 at 2127.770 kb on + strand, within DZA65_RS10050 at 2127.837 kb on + strand, within DZA65_RS10050 at 2127.838 kb on - strand, within DZA65_RS10050 at 2128.057 kb on + strand at 2128.058 kb on - strand at 2128.077 kb on + strand at 2128.078 kb on - strand at 2128.215 kb on + strand at 2128.258 kb on + strand, within DZA65_RS10055 at 2128.258 kb on + strand, within DZA65_RS10055 at 2128.342 kb on - strand, within DZA65_RS10055 at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.753 kb on + strand at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.754 kb on - strand, within DZA65_RS10055 at 2128.754 kb on - strand, within DZA65_RS10055 at 2129.000 kb on + strand, within DZA65_RS10055 at 2129.002 kb on + strand, within DZA65_RS10055 at 2129.002 kb on + strand, within DZA65_RS10055 at 2129.002 kb on + strand, within DZA65_RS10055 at 2129.003 kb on - strand, within DZA65_RS10055 at 2129.003 kb on - strand, within DZA65_RS10055
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,126,163 + DZA65_RS10045 0.21 -0.4 2,126,163 + DZA65_RS10045 0.21 -0.8 2,126,163 + DZA65_RS10045 0.21 -2.8 2,126,164 - DZA65_RS10045 0.21 +0.6 2,126,331 + DZA65_RS10045 0.37 -1.3 2,126,332 - DZA65_RS10045 0.37 -0.3 2,126,545 + DZA65_RS10045 0.57 +0.2 2,126,545 + DZA65_RS10045 0.57 -0.4 2,126,546 - DZA65_RS10045 0.57 -3.0 2,126,628 + DZA65_RS10045 0.65 -1.6 2,126,628 + DZA65_RS10045 0.65 -0.2 2,126,628 + DZA65_RS10045 0.65 +0.2 2,126,629 - DZA65_RS10045 0.65 +0.5 2,126,992 - -0.3 2,126,992 - -2.4 2,126,992 - -1.7 2,127,020 + +0.2 2,127,020 + +0.2 2,127,021 - -0.7 2,127,021 - -0.8 2,127,221 + DZA65_RS10050 0.19 -1.4 2,127,222 - DZA65_RS10050 0.19 -0.8 2,127,223 + DZA65_RS10050 0.19 -1.4 2,127,232 + +0.2 2,127,232 + DZA65_RS10050 0.20 -0.4 2,127,539 + DZA65_RS10050 0.49 -2.4 2,127,540 - DZA65_RS10050 0.49 -0.3 2,127,540 - DZA65_RS10050 0.49 +1.2 2,127,647 + DZA65_RS10050 0.59 -1.3 2,127,647 + DZA65_RS10050 0.59 -1.8 2,127,647 + DZA65_RS10050 0.59 -2.4 2,127,648 - DZA65_RS10050 0.59 -0.5 2,127,762 - DZA65_RS10050 0.69 +0.5 2,127,770 + DZA65_RS10050 0.70 +0.1 2,127,837 + DZA65_RS10050 0.76 -2.8 2,127,838 - DZA65_RS10050 0.76 -2.1 2,128,057 + -0.2 2,128,058 - -1.6 2,128,077 + -2.0 2,128,078 - -2.1 2,128,215 + +0.2 2,128,258 + DZA65_RS10055 0.13 -1.4 2,128,258 + DZA65_RS10055 0.13 -0.2 2,128,342 - DZA65_RS10055 0.20 -0.1 2,128,753 + DZA65_RS10055 0.51 -1.5 2,128,753 + +1.2 2,128,753 + DZA65_RS10055 0.51 -1.1 2,128,753 + DZA65_RS10055 0.51 -1.4 2,128,753 + DZA65_RS10055 0.51 -0.1 2,128,754 - DZA65_RS10055 0.51 -2.8 2,128,754 - DZA65_RS10055 0.51 +0.5 2,129,000 + DZA65_RS10055 0.70 +0.9 2,129,002 + DZA65_RS10055 0.70 -0.3 2,129,002 + DZA65_RS10055 0.70 -0.9 2,129,002 + DZA65_RS10055 0.70 -3.7 2,129,003 - DZA65_RS10055 0.70 -0.5 2,129,003 - DZA65_RS10055 0.70 -3.1
Or see this region's nucleotide sequence