Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01265 and DZA65_RS01270 are separated by 82 nucleotides DZA65_RS01270 and DZA65_RS01275 are separated by 185 nucleotides DZA65_RS01275 and DZA65_RS01280 are separated by 102 nucleotides DZA65_RS01280 and DZA65_RS01285 are separated by 280 nucleotides
DZA65_RS01265: DZA65_RS01265 - 16S ribosomal RNA, at 282,076 to 283,623
_RS01265
DZA65_RS01270: DZA65_RS01270 - tRNA-Glu, at 283,706 to 283,781
_RS01270
DZA65_RS01275: DZA65_RS01275 - 23S ribosomal RNA, at 283,967 to 286,990
_RS01275
DZA65_RS01280: DZA65_RS01280 - 5S ribosomal RNA, at 287,093 to 287,208
_RS01280
DZA65_RS01285: DZA65_RS01285 - amino acid ABC transporter ATP-binding protein, at 287,489 to 288,250
_RS01285
Position (kb) 283
284
285
286
287 Strain fitness (log2 ratio) -2
-1
0
1 at 285.328 kb on + strand, within DZA65_RS01275 at 285.328 kb on + strand, within DZA65_RS01275 at 285.328 kb on + strand, within DZA65_RS01275 at 285.328 kb on + strand, within DZA65_RS01275 at 285.328 kb on + strand, within DZA65_RS01275 at 286.144 kb on + strand, within DZA65_RS01275 at 287.323 kb on - strand at 287.458 kb on - strand at 287.458 kb on - strand at 287.458 kb on - strand at 287.491 kb on - strand at 287.499 kb on - strand at 287.499 kb on - strand at 287.521 kb on + strand at 287.521 kb on + strand at 287.521 kb on + strand at 287.522 kb on - strand at 287.522 kb on - strand at 287.522 kb on - strand at 287.591 kb on - strand, within DZA65_RS01285 at 287.591 kb on - strand, within DZA65_RS01285 at 287.591 kb on - strand, within DZA65_RS01285 at 287.614 kb on + strand, within DZA65_RS01285 at 287.821 kb on + strand, within DZA65_RS01285 at 287.822 kb on - strand, within DZA65_RS01285 at 287.822 kb on - strand, within DZA65_RS01285 at 287.822 kb on - strand, within DZA65_RS01285 at 287.822 kb on - strand, within DZA65_RS01285 at 287.856 kb on + strand, within DZA65_RS01285 at 287.856 kb on + strand, within DZA65_RS01285 at 287.856 kb on + strand, within DZA65_RS01285 at 287.857 kb on - strand, within DZA65_RS01285 at 287.857 kb on - strand, within DZA65_RS01285 at 287.920 kb on + strand, within DZA65_RS01285 at 287.920 kb on + strand, within DZA65_RS01285 at 287.921 kb on - strand, within DZA65_RS01285 at 287.921 kb on - strand, within DZA65_RS01285
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 285,328 + DZA65_RS01275 0.45 +0.6 285,328 + DZA65_RS01275 0.45 -2.2 285,328 + DZA65_RS01275 0.45 -0.4 285,328 + DZA65_RS01275 0.45 -0.1 285,328 + DZA65_RS01275 0.45 -0.6 286,144 + DZA65_RS01275 0.72 -0.5 287,323 - -1.3 287,458 - -1.3 287,458 - -2.2 287,458 - +0.3 287,491 - +0.1 287,499 - +0.9 287,499 - +0.2 287,521 + +1.0 287,521 + -0.9 287,521 + -1.0 287,522 - +1.2 287,522 - +0.7 287,522 - -0.1 287,591 - DZA65_RS01285 0.13 +0.2 287,591 - DZA65_RS01285 0.13 -2.2 287,591 - DZA65_RS01285 0.13 +1.1 287,614 + DZA65_RS01285 0.16 -0.7 287,821 + DZA65_RS01285 0.44 -0.4 287,822 - DZA65_RS01285 0.44 +1.0 287,822 - DZA65_RS01285 0.44 -0.8 287,822 - DZA65_RS01285 0.44 +1.0 287,822 - DZA65_RS01285 0.44 +0.2 287,856 + DZA65_RS01285 0.48 +0.4 287,856 + DZA65_RS01285 0.48 -0.8 287,856 + DZA65_RS01285 0.48 -0.2 287,857 - DZA65_RS01285 0.48 +0.1 287,857 - DZA65_RS01285 0.48 +1.5 287,920 + DZA65_RS01285 0.57 -0.1 287,920 + DZA65_RS01285 0.57 -1.2 287,921 - DZA65_RS01285 0.57 +0.5 287,921 - DZA65_RS01285 0.57 -0.3
Or see this region's nucleotide sequence