Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01010 and DZA65_RS01015 are separated by 138 nucleotides DZA65_RS01015 and DZA65_RS01020 are separated by 36 nucleotides DZA65_RS01020 and DZA65_RS01025 are separated by 25 nucleotides DZA65_RS01025 and DZA65_RS01030 are separated by 52 nucleotides
DZA65_RS01010: DZA65_RS01010 - FAD-dependent oxidoreductase, at 229,372 to 231,045
_RS01010
DZA65_RS01015: DZA65_RS01015 - helix-turn-helix transcriptional regulator, at 231,184 to 231,501
_RS01015
DZA65_RS01020: DZA65_RS01020 - YeeE/YedE family protein, at 231,538 to 231,966
_RS01020
DZA65_RS01025: DZA65_RS01025 - membrane protein, at 231,992 to 232,420
_RS01025
DZA65_RS01030: DZA65_RS01030 - acetyl-CoA C-acyltransferase FadA, at 232,473 to 233,636
_RS01030
Position (kb)
231
232
233 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 231.033 kb on + strand at 231.033 kb on + strand at 231.033 kb on + strand at 231.033 kb on + strand at 231.033 kb on + strand at 231.034 kb on - strand at 231.034 kb on - strand at 231.034 kb on - strand at 231.065 kb on - strand at 231.098 kb on + strand at 231.183 kb on + strand at 231.438 kb on + strand, within DZA65_RS01015 at 231.439 kb on - strand, within DZA65_RS01015 at 231.468 kb on + strand, within DZA65_RS01015 at 231.468 kb on + strand, within DZA65_RS01015 at 231.468 kb on + strand, within DZA65_RS01015 at 231.468 kb on + strand, within DZA65_RS01015 at 231.469 kb on - strand, within DZA65_RS01015 at 231.469 kb on - strand, within DZA65_RS01015 at 231.469 kb on - strand, within DZA65_RS01015 at 231.469 kb on - strand, within DZA65_RS01015 at 231.469 kb on - strand, within DZA65_RS01015 at 231.471 kb on + strand at 231.471 kb on + strand at 231.471 kb on + strand at 231.471 kb on + strand at 231.472 kb on - strand at 231.472 kb on - strand at 231.472 kb on - strand at 231.472 kb on - strand at 231.472 kb on - strand at 231.478 kb on + strand at 231.478 kb on + strand at 231.478 kb on + strand at 231.479 kb on - strand at 231.479 kb on - strand at 231.479 kb on - strand at 231.479 kb on - strand at 231.479 kb on - strand at 231.487 kb on + strand at 231.487 kb on + strand at 231.487 kb on + strand at 231.487 kb on + strand at 231.487 kb on + strand at 231.487 kb on + strand at 231.487 kb on + strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.499 kb on + strand at 231.499 kb on + strand at 231.499 kb on + strand at 231.500 kb on - strand at 231.500 kb on - strand at 231.500 kb on - strand at 231.549 kb on + strand at 231.550 kb on - strand at 231.550 kb on - strand at 231.588 kb on + strand, within DZA65_RS01020 at 231.589 kb on - strand, within DZA65_RS01020 at 231.624 kb on - strand, within DZA65_RS01020 at 231.776 kb on + strand, within DZA65_RS01020 at 232.002 kb on + strand at 232.002 kb on + strand at 232.002 kb on + strand at 232.002 kb on + strand at 232.003 kb on - strand at 232.003 kb on - strand at 232.003 kb on - strand at 232.091 kb on - strand, within DZA65_RS01025 at 232.219 kb on - strand, within DZA65_RS01025 at 232.234 kb on + strand, within DZA65_RS01025 at 232.323 kb on - strand, within DZA65_RS01025 at 232.323 kb on - strand, within DZA65_RS01025 at 232.379 kb on + strand at 232.379 kb on + strand at 232.379 kb on + strand at 232.379 kb on + strand at 232.379 kb on + strand at 232.379 kb on + strand at 232.380 kb on - strand at 232.380 kb on - strand at 232.380 kb on - strand at 232.380 kb on - strand at 232.380 kb on - strand at 232.380 kb on - strand at 232.393 kb on + strand at 232.474 kb on + strand at 232.475 kb on - strand at 232.476 kb on + strand at 232.477 kb on - strand at 232.517 kb on + strand at 232.518 kb on - strand at 232.518 kb on - strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.524 kb on - strand at 232.524 kb on - strand at 232.524 kb on - strand at 232.524 kb on - strand at 232.524 kb on - strand at 232.524 kb on - strand at 232.524 kb on - strand at 232.565 kb on + strand at 232.646 kb on + strand, within DZA65_RS01030 at 232.740 kb on + strand, within DZA65_RS01030 at 232.740 kb on + strand, within DZA65_RS01030 at 232.761 kb on + strand, within DZA65_RS01030 at 232.762 kb on - strand, within DZA65_RS01030 at 232.762 kb on - strand, within DZA65_RS01030 at 232.762 kb on - strand, within DZA65_RS01030 at 232.991 kb on + strand, within DZA65_RS01030 at 233.021 kb on - strand, within DZA65_RS01030 at 233.069 kb on + strand, within DZA65_RS01030 at 233.213 kb on + strand, within DZA65_RS01030 at 233.416 kb on - strand, within DZA65_RS01030 at 233.416 kb on - strand, within DZA65_RS01030
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 231,033 + -0.3 231,033 + +0.2 231,033 + -0.8 231,033 + +0.2 231,033 + +0.4 231,034 - -0.8 231,034 - -0.5 231,034 - -1.5 231,065 - +0.1 231,098 + -0.2 231,183 + +0.1 231,438 + DZA65_RS01015 0.80 -1.0 231,439 - DZA65_RS01015 0.80 -0.8 231,468 + DZA65_RS01015 0.89 -0.8 231,468 + DZA65_RS01015 0.89 +1.1 231,468 + DZA65_RS01015 0.89 +0.6 231,468 + DZA65_RS01015 0.89 -0.5 231,469 - DZA65_RS01015 0.90 +0.6 231,469 - DZA65_RS01015 0.90 -0.0 231,469 - DZA65_RS01015 0.90 +0.4 231,469 - DZA65_RS01015 0.90 +0.1 231,469 - DZA65_RS01015 0.90 +0.4 231,471 + -0.9 231,471 + -1.3 231,471 + -0.2 231,471 + +0.5 231,472 - -0.4 231,472 - -2.2 231,472 - -1.3 231,472 - +0.6 231,472 - +0.6 231,478 + -1.4 231,478 + -0.5 231,478 + +1.4 231,479 - -0.2 231,479 - -0.4 231,479 - +0.2 231,479 - +0.4 231,479 - -0.1 231,487 + -0.1 231,487 + +0.3 231,487 + +0.0 231,487 + +0.6 231,487 + -1.4 231,487 + -0.2 231,487 + +0.3 231,488 - +1.0 231,488 - -0.5 231,488 - +0.6 231,488 - +0.5 231,488 - +1.1 231,488 - +0.8 231,488 - +0.6 231,488 - +0.5 231,488 - -2.6 231,499 + +1.7 231,499 + +0.0 231,499 + -1.2 231,500 - +0.1 231,500 - +0.6 231,500 - +0.4 231,549 + -1.4 231,550 - +0.1 231,550 - +0.3 231,588 + DZA65_RS01020 0.12 -0.2 231,589 - DZA65_RS01020 0.12 -0.4 231,624 - DZA65_RS01020 0.20 +0.0 231,776 + DZA65_RS01020 0.55 +0.3 232,002 + -1.2 232,002 + +0.3 232,002 + +0.9 232,002 + -1.7 232,003 - +1.1 232,003 - +0.3 232,003 - +1.2 232,091 - DZA65_RS01025 0.23 +0.3 232,219 - DZA65_RS01025 0.53 -0.2 232,234 + DZA65_RS01025 0.56 +1.9 232,323 - DZA65_RS01025 0.77 +0.8 232,323 - DZA65_RS01025 0.77 -0.3 232,379 + -2.9 232,379 + +0.4 232,379 + +0.5 232,379 + -0.6 232,379 + -0.3 232,379 + +0.9 232,380 - +0.5 232,380 - +0.3 232,380 - +1.3 232,380 - -0.1 232,380 - +0.5 232,380 - -2.2 232,393 + +0.3 232,474 + +0.4 232,475 - -2.4 232,476 + -1.0 232,477 - -0.1 232,517 + +0.6 232,518 - +0.3 232,518 - +0.6 232,523 + -0.1 232,523 + +0.8 232,523 + -0.2 232,523 + -0.1 232,523 + -0.3 232,523 + +0.3 232,523 + -0.1 232,523 + +0.3 232,523 + -1.0 232,523 + +0.1 232,523 + +1.6 232,524 - -0.4 232,524 - -0.1 232,524 - -0.4 232,524 - -0.3 232,524 - +0.6 232,524 - -0.6 232,524 - +0.2 232,565 + -1.0 232,646 + DZA65_RS01030 0.15 -1.7 232,740 + DZA65_RS01030 0.23 -0.7 232,740 + DZA65_RS01030 0.23 -0.4 232,761 + DZA65_RS01030 0.25 +0.1 232,762 - DZA65_RS01030 0.25 +1.2 232,762 - DZA65_RS01030 0.25 +0.8 232,762 - DZA65_RS01030 0.25 -0.7 232,991 + DZA65_RS01030 0.45 +0.3 233,021 - DZA65_RS01030 0.47 -1.5 233,069 + DZA65_RS01030 0.51 +0.9 233,213 + DZA65_RS01030 0.64 +1.2 233,416 - DZA65_RS01030 0.81 -2.4 233,416 - DZA65_RS01030 0.81 +0.6
Or see this region's nucleotide sequence