Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01170 and DZA65_RS01175 are separated by 275 nucleotides DZA65_RS01175 and DZA65_RS01180 are separated by 259 nucleotides DZA65_RS01180 and DZA65_RS22770 are separated by 24 nucleotides DZA65_RS22770 and DZA65_RS01185 overlap by 4 nucleotides
DZA65_RS01170: DZA65_RS01170 - DUF1176 domain-containing protein, at 263,862 to 264,959
_RS01170
DZA65_RS01175: DZA65_RS01175 - DUF1127 domain-containing protein, at 265,235 to 265,414
_RS01175
DZA65_RS01180: DZA65_RS01180 - DUF1127 domain-containing protein, at 265,674 to 265,865
_RS01180
DZA65_RS22770: DZA65_RS22770 - hypothetical protein, at 265,890 to 266,033
_RS22770
DZA65_RS01185: DZA65_RS01185 - PLP-dependent aminotransferase family protein, at 266,030 to 267,460
_RS01185
Position (kb)
265
266
267 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 264.896 kb on + strand at 264.896 kb on + strand at 264.896 kb on + strand at 264.897 kb on - strand at 264.897 kb on - strand at 265.039 kb on - strand at 265.039 kb on - strand at 265.057 kb on + strand at 265.074 kb on + strand at 265.074 kb on + strand at 265.075 kb on - strand at 265.075 kb on - strand at 265.075 kb on - strand at 265.075 kb on - strand at 265.143 kb on - strand at 265.143 kb on - strand at 265.143 kb on - strand at 265.151 kb on + strand at 265.151 kb on + strand at 265.152 kb on - strand at 265.152 kb on - strand at 265.154 kb on + strand at 265.208 kb on + strand at 265.297 kb on + strand, within DZA65_RS01175 at 265.297 kb on + strand, within DZA65_RS01175 at 265.297 kb on + strand, within DZA65_RS01175 at 265.297 kb on + strand, within DZA65_RS01175 at 265.297 kb on + strand, within DZA65_RS01175 at 265.298 kb on - strand, within DZA65_RS01175 at 265.298 kb on - strand, within DZA65_RS01175 at 265.301 kb on + strand, within DZA65_RS01175 at 265.301 kb on + strand, within DZA65_RS01175 at 265.301 kb on + strand, within DZA65_RS01175 at 265.302 kb on - strand, within DZA65_RS01175 at 265.302 kb on - strand, within DZA65_RS01175 at 265.302 kb on - strand, within DZA65_RS01175 at 265.302 kb on - strand, within DZA65_RS01175 at 265.344 kb on + strand, within DZA65_RS01175 at 265.344 kb on + strand, within DZA65_RS01175 at 265.401 kb on + strand at 265.401 kb on + strand at 265.421 kb on - strand at 265.439 kb on + strand at 265.440 kb on - strand at 265.440 kb on - strand at 265.442 kb on + strand at 265.442 kb on + strand at 265.442 kb on + strand at 265.442 kb on + strand at 265.443 kb on - strand at 265.443 kb on - strand at 265.443 kb on - strand at 265.443 kb on - strand at 265.472 kb on + strand at 265.472 kb on + strand at 265.502 kb on + strand at 265.503 kb on - strand at 265.518 kb on + strand at 265.519 kb on - strand at 265.632 kb on - strand at 265.650 kb on + strand at 265.650 kb on + strand at 265.651 kb on - strand at 265.651 kb on - strand at 265.718 kb on + strand, within DZA65_RS01180 at 265.745 kb on + strand, within DZA65_RS01180 at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.855 kb on - strand at 265.855 kb on - strand at 265.855 kb on - strand at 265.855 kb on - strand at 265.855 kb on - strand at 265.857 kb on + strand at 265.857 kb on + strand at 265.857 kb on + strand at 265.857 kb on + strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.916 kb on + strand, within DZA65_RS22770 at 265.963 kb on + strand, within DZA65_RS22770 at 265.963 kb on + strand, within DZA65_RS22770 at 265.963 kb on + strand, within DZA65_RS22770 at 265.964 kb on - strand, within DZA65_RS22770 at 265.964 kb on - strand, within DZA65_RS22770 at 265.964 kb on - strand, within DZA65_RS22770 at 266.001 kb on + strand, within DZA65_RS22770 at 266.023 kb on + strand at 266.023 kb on + strand at 266.023 kb on + strand at 266.024 kb on - strand at 266.024 kb on - strand at 266.029 kb on + strand at 266.029 kb on + strand at 266.030 kb on - strand at 266.030 kb on - strand at 266.030 kb on - strand at 266.058 kb on + strand at 266.087 kb on + strand at 266.087 kb on + strand at 266.087 kb on + strand at 266.088 kb on - strand at 266.115 kb on + strand at 266.116 kb on - strand at 266.124 kb on + strand at 266.124 kb on + strand at 266.124 kb on + strand at 266.146 kb on + strand at 266.146 kb on + strand at 266.168 kb on + strand at 266.169 kb on - strand at 266.169 kb on - strand at 266.169 kb on - strand at 266.169 kb on - strand at 266.169 kb on - strand at 266.227 kb on + strand, within DZA65_RS01185 at 266.400 kb on + strand, within DZA65_RS01185 at 266.410 kb on + strand, within DZA65_RS01185 at 266.411 kb on - strand, within DZA65_RS01185 at 266.472 kb on + strand, within DZA65_RS01185 at 266.472 kb on + strand, within DZA65_RS01185 at 266.560 kb on + strand, within DZA65_RS01185 at 266.560 kb on + strand, within DZA65_RS01185 at 266.596 kb on + strand, within DZA65_RS01185 at 266.596 kb on + strand, within DZA65_RS01185 at 266.596 kb on + strand, within DZA65_RS01185 at 266.597 kb on - strand, within DZA65_RS01185 at 266.597 kb on - strand, within DZA65_RS01185 at 266.597 kb on - strand, within DZA65_RS01185 at 266.653 kb on - strand, within DZA65_RS01185 at 266.653 kb on - strand, within DZA65_RS01185 at 266.653 kb on - strand, within DZA65_RS01185 at 266.876 kb on + strand, within DZA65_RS01185 at 266.876 kb on + strand, within DZA65_RS01185 at 266.876 kb on + strand, within DZA65_RS01185 at 266.877 kb on - strand, within DZA65_RS01185 at 266.877 kb on - strand, within DZA65_RS01185 at 266.877 kb on - strand, within DZA65_RS01185 at 266.877 kb on - strand, within DZA65_RS01185 at 266.891 kb on + strand at 266.891 kb on + strand, within DZA65_RS01185 at 266.891 kb on + strand, within DZA65_RS01185 at 266.891 kb on + strand, within DZA65_RS01185 at 266.891 kb on + strand, within DZA65_RS01185 at 266.891 kb on + strand, within DZA65_RS01185 at 266.891 kb on + strand, within DZA65_RS01185 at 266.891 kb on + strand, within DZA65_RS01185 at 266.891 kb on + strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.897 kb on + strand, within DZA65_RS01185 at 266.897 kb on + strand, within DZA65_RS01185 at 266.898 kb on - strand, within DZA65_RS01185 at 266.898 kb on - strand, within DZA65_RS01185 at 266.898 kb on - strand, within DZA65_RS01185 at 266.911 kb on + strand, within DZA65_RS01185 at 266.912 kb on - strand, within DZA65_RS01185 at 266.912 kb on - strand, within DZA65_RS01185
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 264,896 + -1.1 264,896 + -0.4 264,896 + +1.0 264,897 - -0.7 264,897 - +1.0 265,039 - -0.1 265,039 - +0.1 265,057 + -0.5 265,074 + +0.9 265,074 + +1.4 265,075 - -0.4 265,075 - +0.7 265,075 - -1.1 265,075 - +0.5 265,143 - -1.1 265,143 - -0.1 265,143 - +1.2 265,151 + -0.3 265,151 + -2.4 265,152 - +0.4 265,152 - -1.3 265,154 + -1.3 265,208 + -1.9 265,297 + DZA65_RS01175 0.34 +1.7 265,297 + DZA65_RS01175 0.34 -1.1 265,297 + DZA65_RS01175 0.34 +1.3 265,297 + DZA65_RS01175 0.34 +1.2 265,297 + DZA65_RS01175 0.34 +0.0 265,298 - DZA65_RS01175 0.35 -0.6 265,298 - DZA65_RS01175 0.35 +0.1 265,301 + DZA65_RS01175 0.37 -0.6 265,301 + DZA65_RS01175 0.37 -0.3 265,301 + DZA65_RS01175 0.37 -0.1 265,302 - DZA65_RS01175 0.37 -0.4 265,302 - DZA65_RS01175 0.37 -0.3 265,302 - DZA65_RS01175 0.37 +0.0 265,302 - DZA65_RS01175 0.37 -0.9 265,344 + DZA65_RS01175 0.61 -0.4 265,344 + DZA65_RS01175 0.61 -0.3 265,401 + -1.2 265,401 + -0.8 265,421 - -2.4 265,439 + +0.4 265,440 - -0.7 265,440 - -0.9 265,442 + -1.3 265,442 + -0.0 265,442 + +0.4 265,442 + +0.7 265,443 - -0.4 265,443 - -1.3 265,443 - -0.1 265,443 - -1.1 265,472 + -1.3 265,472 + -0.6 265,502 + +0.3 265,503 - -1.5 265,518 + -0.7 265,519 - +0.3 265,632 - -1.6 265,650 + -1.3 265,650 + -1.0 265,651 - -0.4 265,651 - -1.2 265,718 + DZA65_RS01180 0.23 +0.5 265,745 + DZA65_RS01180 0.37 -1.1 265,854 + +0.3 265,854 + +0.1 265,854 + -0.0 265,854 + -1.4 265,854 + -1.7 265,854 + -1.4 265,854 + -0.8 265,854 + -1.1 265,854 + -0.4 265,854 + -0.1 265,854 + -0.5 265,854 + -0.1 265,854 + +1.4 265,855 - -1.4 265,855 - +0.0 265,855 - -1.3 265,855 - -0.3 265,855 - -0.4 265,857 + +0.1 265,857 + -0.3 265,857 + +1.0 265,857 + -0.1 265,858 - -2.1 265,858 - -0.4 265,858 - -0.1 265,858 - -0.7 265,858 - -2.2 265,858 - -0.6 265,858 - -1.6 265,858 - +0.1 265,893 + -0.8 265,893 + -0.4 265,893 + -0.6 265,893 + +2.3 265,893 + -0.5 265,893 + +2.3 265,893 + +0.9 265,894 - -1.0 265,894 - -0.2 265,894 - +1.0 265,894 - -0.3 265,894 - -1.9 265,894 - +0.0 265,894 - +0.3 265,894 - -0.3 265,894 - -0.1 265,894 - -1.2 265,894 - -0.6 265,916 + DZA65_RS22770 0.18 -2.3 265,963 + DZA65_RS22770 0.51 -1.4 265,963 + DZA65_RS22770 0.51 -1.1 265,963 + DZA65_RS22770 0.51 +2.1 265,964 - DZA65_RS22770 0.51 -2.7 265,964 - DZA65_RS22770 0.51 -0.8 265,964 - DZA65_RS22770 0.51 -1.9 266,001 + DZA65_RS22770 0.77 -0.3 266,023 + +0.3 266,023 + -1.6 266,023 + -0.7 266,024 - -0.3 266,024 - -0.4 266,029 + -1.3 266,029 + -1.2 266,030 - +1.1 266,030 - -0.6 266,030 - -1.4 266,058 + +0.4 266,087 + +0.3 266,087 + -2.0 266,087 + -1.5 266,088 - -1.3 266,115 + -0.3 266,116 - +0.1 266,124 + -1.5 266,124 + -1.9 266,124 + -1.1 266,146 + +0.7 266,146 + -1.5 266,168 + -0.7 266,169 - -1.0 266,169 - +0.7 266,169 - +0.1 266,169 - +0.1 266,169 - +0.7 266,227 + DZA65_RS01185 0.14 -0.4 266,400 + DZA65_RS01185 0.26 -0.3 266,410 + DZA65_RS01185 0.27 -0.5 266,411 - DZA65_RS01185 0.27 -0.8 266,472 + DZA65_RS01185 0.31 -1.4 266,472 + DZA65_RS01185 0.31 -0.1 266,560 + DZA65_RS01185 0.37 -1.4 266,560 + DZA65_RS01185 0.37 +1.1 266,596 + DZA65_RS01185 0.40 +0.4 266,596 + DZA65_RS01185 0.40 -0.9 266,596 + DZA65_RS01185 0.40 -0.9 266,597 - DZA65_RS01185 0.40 -2.1 266,597 - DZA65_RS01185 0.40 +0.1 266,597 - DZA65_RS01185 0.40 -0.3 266,653 - DZA65_RS01185 0.44 -0.5 266,653 - DZA65_RS01185 0.44 -1.0 266,653 - DZA65_RS01185 0.44 -0.4 266,876 + DZA65_RS01185 0.59 -1.2 266,876 + DZA65_RS01185 0.59 -0.9 266,876 + DZA65_RS01185 0.59 +0.4 266,877 - DZA65_RS01185 0.59 +0.5 266,877 - DZA65_RS01185 0.59 +0.1 266,877 - DZA65_RS01185 0.59 +0.3 266,877 - DZA65_RS01185 0.59 +0.7 266,891 + +1.7 266,891 + DZA65_RS01185 0.60 +0.7 266,891 + DZA65_RS01185 0.60 -0.0 266,891 + DZA65_RS01185 0.60 -1.4 266,891 + DZA65_RS01185 0.60 -0.0 266,891 + DZA65_RS01185 0.60 +0.7 266,891 + DZA65_RS01185 0.60 -0.9 266,891 + DZA65_RS01185 0.60 -0.2 266,891 + DZA65_RS01185 0.60 +0.4 266,892 - DZA65_RS01185 0.60 +0.6 266,892 - DZA65_RS01185 0.60 -1.3 266,892 - DZA65_RS01185 0.60 -1.2 266,892 - DZA65_RS01185 0.60 -1.4 266,892 - DZA65_RS01185 0.60 +0.5 266,892 - DZA65_RS01185 0.60 -0.7 266,892 - DZA65_RS01185 0.60 +0.4 266,892 - DZA65_RS01185 0.60 +1.1 266,892 - DZA65_RS01185 0.60 +0.6 266,897 + DZA65_RS01185 0.61 -0.6 266,897 + DZA65_RS01185 0.61 +0.4 266,898 - DZA65_RS01185 0.61 +0.1 266,898 - DZA65_RS01185 0.61 -0.1 266,898 - DZA65_RS01185 0.61 +0.7 266,911 + DZA65_RS01185 0.62 -1.7 266,912 - DZA65_RS01185 0.62 -0.3 266,912 - DZA65_RS01185 0.62 -0.4
Or see this region's nucleotide sequence