Strain Fitness in Dickeya dianthicola ME23

Strain Fitness in Dickeya dianthicola ME23 around DZA65_RS22085

Experiment: LB

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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Per-strain Table

Position	Strand	LocusTag	Fraction	LB
				remove
4,773,540	-	DZA65_RS22080	0.23	-0.8
4,773,540	-	DZA65_RS22080	0.23	-0.6
4,773,549	+	DZA65_RS22080	0.24	+0.1
4,773,636	+	DZA65_RS22080	0.31	+0.8
4,773,637	-	DZA65_RS22080	0.31	+0.2
4,773,713	+	DZA65_RS22080	0.37	-0.0
4,773,713	+	DZA65_RS22080	0.37	-1.5
4,773,713	+	DZA65_RS22080	0.37	-0.8
4,773,714	-	DZA65_RS22080	0.37	-0.3
4,773,753	+	DZA65_RS22080	0.40	+0.0
4,773,753	+			+0.8
4,773,753	+	DZA65_RS22080	0.40	-0.7
4,773,754	-	DZA65_RS22080	0.40	+0.6
4,773,754	-	DZA65_RS22080	0.40	-0.3
4,773,754	-	DZA65_RS22080	0.40	+0.7
4,773,754	-	DZA65_RS22080	0.40	+0.1
4,773,754	-	DZA65_RS22080	0.40	-0.1
4,773,754	-	DZA65_RS22080	0.40	-0.2
4,773,754	-	DZA65_RS22080	0.40	+0.1
4,773,754	-	DZA65_RS22080	0.40	-0.2
4,773,754	-	DZA65_RS22080	0.40	-0.9
4,773,755	+	DZA65_RS22080	0.40	-0.0
4,773,755	+	DZA65_RS22080	0.40	-0.8
4,773,755	+	DZA65_RS22080	0.40	-1.4
4,773,755	+	DZA65_RS22080	0.40	-0.2
4,773,756	-	DZA65_RS22080	0.40	-1.1
4,773,756	-	DZA65_RS22080	0.40	-1.0
4,773,756	-	DZA65_RS22080	0.40	-2.1
4,773,762	-	DZA65_RS22080	0.41	+0.1
4,773,762	-	DZA65_RS22080	0.41	+0.2
4,773,785	+	DZA65_RS22080	0.42	-0.5
4,773,785	+	DZA65_RS22080	0.42	-0.2
4,773,785	+	DZA65_RS22080	0.42	-0.1
4,773,785	+	DZA65_RS22080	0.42	+1.2
4,773,785	+	DZA65_RS22080	0.42	+0.0
4,773,785	+	DZA65_RS22080	0.42	+0.3
4,773,786	-	DZA65_RS22080	0.43	+0.2
4,773,786	-	DZA65_RS22080	0.43	-0.8
4,773,786	-	DZA65_RS22080	0.43	-0.2
4,773,789	+	DZA65_RS22080	0.43	+0.8
4,773,789	+	DZA65_RS22080	0.43	-0.9
4,773,789	+	DZA65_RS22080	0.43	+0.5
4,773,789	+	DZA65_RS22080	0.43	-0.8
4,773,789	+	DZA65_RS22080	0.43	-1.6
4,773,789	+	DZA65_RS22080	0.43	-1.5
4,773,789	+	DZA65_RS22080	0.43	-1.3
4,773,789	+	DZA65_RS22080	0.43	+0.0
4,773,790	-	DZA65_RS22080	0.43	-1.9
4,773,790	-	DZA65_RS22080	0.43	+1.2
4,773,790	-	DZA65_RS22080	0.43	-1.4
4,773,790	-	DZA65_RS22080	0.43	-1.0
4,773,790	-	DZA65_RS22080	0.43	+1.2
4,773,790	-	DZA65_RS22080	0.43	+1.1
4,773,790	-	DZA65_RS22080	0.43	-0.5
4,773,790	-	DZA65_RS22080	0.43	+0.4
4,773,790	-	DZA65_RS22080	0.43	-0.2
4,773,790	-	DZA65_RS22080	0.43	+0.5
4,773,790	-	DZA65_RS22080	0.43	-1.1
4,773,790	-	DZA65_RS22080	0.43	-0.4
4,773,790	-	DZA65_RS22080	0.43	-0.1
4,773,959	+	DZA65_RS22080	0.56	+0.2
4,773,959	+	DZA65_RS22080	0.56	-1.1
4,773,959	+	DZA65_RS22080	0.56	-0.2
4,773,960	-	DZA65_RS22080	0.56	+0.8
4,773,960	-	DZA65_RS22080	0.56	-0.2
4,774,076	+	DZA65_RS22080	0.65	+0.2
4,774,076	+	DZA65_RS22080	0.65	+1.2
4,774,076	+	DZA65_RS22080	0.65	+0.1
4,774,077	-	DZA65_RS22080	0.65	-1.6
4,774,077	-	DZA65_RS22080	0.65	-0.4
4,774,079	+	DZA65_RS22080	0.65	-1.3
4,774,079	+	DZA65_RS22080	0.65	-0.1
4,774,079	+	DZA65_RS22080	0.65	-1.9
4,774,079	+	DZA65_RS22080	0.65	+0.2
4,774,079	+	DZA65_RS22080	0.65	-0.7
4,774,080	-	DZA65_RS22080	0.65	-1.0
4,774,080	-	DZA65_RS22080	0.65	+0.8
4,774,080	-	DZA65_RS22080	0.65	+0.0
4,774,080	-	DZA65_RS22080	0.65	+0.4
4,774,080	-	DZA65_RS22080	0.65	+0.0
4,774,080	-	DZA65_RS22080	0.65	-1.8
4,774,080	-	DZA65_RS22080	0.65	-0.3
4,774,080	-	DZA65_RS22080	0.65	-1.2
4,774,115	+	DZA65_RS22080	0.68	-0.5
4,774,185	+	DZA65_RS22080	0.74	-0.8
4,774,193	+	DZA65_RS22080	0.74	-0.8
4,774,193	+	DZA65_RS22080	0.74	-0.8
4,774,193	+	DZA65_RS22080	0.74	-1.2
4,774,193	+	DZA65_RS22080	0.74	-0.8
4,774,193	+	DZA65_RS22080	0.74	-1.6
4,774,193	+	DZA65_RS22080	0.74	+0.5
4,774,193	+	DZA65_RS22080	0.74	-0.8
4,774,193	+	DZA65_RS22080	0.74	+1.2
4,774,193	+	DZA65_RS22080	0.74	-1.0
4,774,193	+	DZA65_RS22080	0.74	-0.6
4,774,194	-	DZA65_RS22080	0.74	+0.9
4,774,194	-	DZA65_RS22080	0.74	-1.3
4,774,194	-	DZA65_RS22080	0.74	-0.2
4,774,194	-	DZA65_RS22080	0.74	+0.4
4,774,194	-	DZA65_RS22080	0.74	-1.8
4,774,194	-	DZA65_RS22080	0.74	+1.1
4,774,194	-	DZA65_RS22080	0.74	-0.5
4,774,194	-	DZA65_RS22080	0.74	+1.8
4,774,319	+	DZA65_RS22080	0.84	+0.1
4,774,319	+	DZA65_RS22080	0.84	-0.1
4,774,319	+	DZA65_RS22080	0.84	-0.9
4,774,319	+	DZA65_RS22080	0.84	+0.4
4,774,319	+	DZA65_RS22080	0.84	-0.8
4,774,320	-	DZA65_RS22080	0.84	-1.6
4,774,320	-	DZA65_RS22080	0.84	-1.5
4,774,320	-	DZA65_RS22080	0.84	-0.6
4,774,320	-	DZA65_RS22080	0.84	-0.3
4,774,363	-	DZA65_RS22080	0.88	-1.5
4,774,363	-	DZA65_RS22080	0.88	+0.2
4,774,363	-	DZA65_RS22080	0.88	-1.4
4,774,363	-	DZA65_RS22080	0.88	-0.3
4,774,369	-	DZA65_RS22080	0.88	-1.1
4,774,369	-	DZA65_RS22080	0.88	-0.6
4,774,410	+			+1.2
4,774,490	-			+1.1
4,774,511	-			-1.0
4,774,522	+			-1.4
4,774,522	+			-0.8
4,774,522	+			-0.1
4,774,523	-			-0.8
4,774,581	+			-1.9
4,774,582	-			-0.8
4,774,582	-			-1.1
4,774,625	+	DZA65_RS22085	0.12	+0.4
4,774,625	+	DZA65_RS22085	0.12	-0.6
4,774,626	-	DZA65_RS22085	0.12	-0.8
4,774,626	-	DZA65_RS22085	0.12	-1.4
4,774,637	+	DZA65_RS22085	0.13	-0.3
4,774,638	-	DZA65_RS22085	0.13	-0.9
4,774,638	-	DZA65_RS22085	0.13	-1.9
4,774,638	-	DZA65_RS22085	0.13	-1.2
4,774,645	+	DZA65_RS22085	0.14	-1.0
4,774,645	+	DZA65_RS22085	0.14	-1.5
4,774,645	+	DZA65_RS22085	0.14	+1.1
4,774,709	+	DZA65_RS22085	0.21	-0.5
4,774,709	+	DZA65_RS22085	0.21	-0.6
4,774,709	+	DZA65_RS22085	0.21	-0.7
4,774,709	+	DZA65_RS22085	0.21	+1.2
4,774,710	-	DZA65_RS22085	0.21	+0.3
4,774,710	-	DZA65_RS22085	0.21	-0.2
4,774,710	-	DZA65_RS22085	0.21	-0.5
4,774,710	-	DZA65_RS22085	0.21	-0.5
4,774,732	+	DZA65_RS22085	0.24	-1.2
4,774,732	+	DZA65_RS22085	0.24	-1.5
4,774,732	+	DZA65_RS22085	0.24	+0.5
4,774,732	+	DZA65_RS22085	0.24	+0.8
4,774,732	+	DZA65_RS22085	0.24	-0.4
4,774,732	+	DZA65_RS22085	0.24	-0.5
4,774,732	+	DZA65_RS22085	0.24	-0.5
4,774,732	+	DZA65_RS22085	0.24	+0.1
4,774,732	+	DZA65_RS22085	0.24	+1.8
4,774,732	+	DZA65_RS22085	0.24	-1.1
4,774,732	+	DZA65_RS22085	0.24	-1.0
4,774,732	+	DZA65_RS22085	0.24	-0.7
4,774,732	+	DZA65_RS22085	0.24	-0.9
4,774,732	+	DZA65_RS22085	0.24	-0.4
4,774,732	+	DZA65_RS22085	0.24	-0.1
4,774,732	+	DZA65_RS22085	0.24	+0.4
4,774,732	+	DZA65_RS22085	0.24	-1.0
4,774,732	+	DZA65_RS22085	0.24	-0.1
4,774,732	+	DZA65_RS22085	0.24	+1.8
4,774,733	-	DZA65_RS22085	0.24	-2.8
4,774,733	-			-0.2
4,774,733	-	DZA65_RS22085	0.24	+0.2
4,774,733	-	DZA65_RS22085	0.24	-1.2
4,774,733	-	DZA65_RS22085	0.24	+0.2
4,774,733	-	DZA65_RS22085	0.24	-0.5
4,774,733	-	DZA65_RS22085	0.24	-0.5
4,774,733	-	DZA65_RS22085	0.24	+0.5
4,774,733	-	DZA65_RS22085	0.24	-0.2
4,774,733	-	DZA65_RS22085	0.24	-1.2
4,774,733	-	DZA65_RS22085	0.24	-1.5
4,774,733	-	DZA65_RS22085	0.24	-0.9
4,774,733	-	DZA65_RS22085	0.24	-0.9
4,774,733	-	DZA65_RS22085	0.24	-1.6
4,774,733	-	DZA65_RS22085	0.24	-0.9
4,774,733	-	DZA65_RS22085	0.24	-1.4
4,774,733	-	DZA65_RS22085	0.24	-1.3
4,774,733	-	DZA65_RS22085	0.24	-0.2
4,774,741	+	DZA65_RS22085	0.25	+0.8
4,774,741	+	DZA65_RS22085	0.25	-3.3
4,774,742	-	DZA65_RS22085	0.25	+0.2
4,774,742	-	DZA65_RS22085	0.25	+0.8
4,774,757	+	DZA65_RS22085	0.26	-2.3
4,774,757	+	DZA65_RS22085	0.26	+0.2
4,774,757	+	DZA65_RS22085	0.26	+0.6
4,774,757	+	DZA65_RS22085	0.26	+0.2
4,774,772	+			+0.8
4,774,788	+	DZA65_RS22085	0.30	-1.5
4,774,789	-	DZA65_RS22085	0.30	-1.0
4,774,789	-	DZA65_RS22085	0.30	-1.1
4,774,789	-	DZA65_RS22085	0.30	+1.2
4,774,808	+	DZA65_RS22085	0.32	-1.5
4,774,809	-	DZA65_RS22085	0.32	-1.0
4,774,809	-	DZA65_RS22085	0.32	+0.4
4,774,841	+	DZA65_RS22085	0.36	-1.0
4,774,841	+	DZA65_RS22085	0.36	-0.7
4,774,842	-	DZA65_RS22085	0.36	-0.3
4,774,842	-	DZA65_RS22085	0.36	-0.8
4,774,842	-	DZA65_RS22085	0.36	-0.2
4,774,857	+	DZA65_RS22085	0.38	+0.8
4,774,890	+	DZA65_RS22085	0.41	-1.9
4,774,890	+	DZA65_RS22085	0.41	+0.0
4,774,890	+	DZA65_RS22085	0.41	+0.3
4,774,890	+	DZA65_RS22085	0.41	+0.8
4,774,895	-	DZA65_RS22085	0.42	-0.5
4,774,895	-	DZA65_RS22085	0.42	-0.5
4,774,978	+	DZA65_RS22085	0.51	+0.5
4,774,978	+	DZA65_RS22085	0.51	-0.3
4,774,978	+	DZA65_RS22085	0.51	-1.2
4,774,978	+	DZA65_RS22085	0.51	-1.4
4,774,979	-	DZA65_RS22085	0.51	+0.2
4,774,979	-	DZA65_RS22085	0.51	-0.5
4,774,979	-	DZA65_RS22085	0.51	+0.2
4,774,979	-	DZA65_RS22085	0.51	-0.4
4,774,979	-	DZA65_RS22085	0.51	-0.5
4,774,989	-	DZA65_RS22085	0.52	-1.0
4,774,990	+	DZA65_RS22085	0.52	-1.2
4,774,990	+	DZA65_RS22085	0.52	-0.2
4,774,990	+	DZA65_RS22085	0.52	-0.2
4,774,990	+	DZA65_RS22085	0.52	-0.2
4,774,990	+	DZA65_RS22085	0.52	+1.2
4,774,990	+	DZA65_RS22085	0.52	-0.3
4,774,990	+	DZA65_RS22085	0.52	+0.8
4,774,990	+	DZA65_RS22085	0.52	+0.5
4,774,990	+	DZA65_RS22085	0.52	-0.3
4,774,990	+	DZA65_RS22085	0.52	-2.4
4,774,990	+	DZA65_RS22085	0.52	-0.2
4,774,991	-	DZA65_RS22085	0.53	-1.9
4,774,991	-	DZA65_RS22085	0.53	+3.4
4,774,991	-	DZA65_RS22085	0.53	-0.2
4,774,991	-	DZA65_RS22085	0.53	-1.1
4,774,991	-	DZA65_RS22085	0.53	-1.6
4,774,991	-	DZA65_RS22085	0.53	+0.1
4,774,991	-	DZA65_RS22085	0.53	-1.4
4,774,991	-	DZA65_RS22085	0.53	-0.1
4,774,991	-	DZA65_RS22085	0.53	-1.5
4,775,012	+	DZA65_RS22085	0.55	-0.1
4,775,012	+	DZA65_RS22085	0.55	+0.7
4,775,012	+	DZA65_RS22085	0.55	+1.2
4,775,012	+	DZA65_RS22085	0.55	+0.4
4,775,012	+	DZA65_RS22085	0.55	-0.6
4,775,012	+	DZA65_RS22085	0.55	+2.8
4,775,012	+	DZA65_RS22085	0.55	+2.2
4,775,012	+	DZA65_RS22085	0.55	-0.8
4,775,012	+	DZA65_RS22085	0.55	-1.4
4,775,012	+	DZA65_RS22085	0.55	-0.5
4,775,013	-	DZA65_RS22085	0.55	-1.0
4,775,013	-	DZA65_RS22085	0.55	+0.8
4,775,013	-	DZA65_RS22085	0.55	-0.6
4,775,013	-	DZA65_RS22085	0.55	+0.0
4,775,013	-	DZA65_RS22085	0.55	+0.4
4,775,013	-	DZA65_RS22085	0.55	+0.2
4,775,013	-	DZA65_RS22085	0.55	+0.3
4,775,013	-	DZA65_RS22085	0.55	-1.1
4,775,013	-	DZA65_RS22085	0.55	-0.5
4,775,013	-	DZA65_RS22085	0.55	-0.4
4,775,013	-	DZA65_RS22085	0.55	-0.8
4,775,013	-			+0.8
4,775,013	-	DZA65_RS22085	0.55	+0.5
4,775,013	-	DZA65_RS22085	0.55	-0.8
4,775,013	-	DZA65_RS22085	0.55	-1.4
4,775,013	-	DZA65_RS22085	0.55	+0.8
4,775,013	-	DZA65_RS22085	0.55	-1.1
4,775,013	-	DZA65_RS22085	0.55	+0.2
4,775,013	-	DZA65_RS22085	0.55	-1.0
4,775,014	+	DZA65_RS22085	0.55	-0.5
4,775,014	+	DZA65_RS22085	0.55	-0.5
4,775,014	+	DZA65_RS22085	0.55	-1.2
4,775,014	+	DZA65_RS22085	0.55	+0.1
4,775,014	+	DZA65_RS22085	0.55	-0.9
4,775,014	+	DZA65_RS22085	0.55	-0.3
4,775,014	+	DZA65_RS22085	0.55	+0.2
4,775,014	+	DZA65_RS22085	0.55	+1.0
4,775,014	+	DZA65_RS22085	0.55	+0.5
4,775,014	+	DZA65_RS22085	0.55	-0.4
4,775,014	+	DZA65_RS22085	0.55	-1.1
4,775,015	-	DZA65_RS22085	0.55	+0.3
4,775,015	-	DZA65_RS22085	0.55	-0.5
4,775,015	-	DZA65_RS22085	0.55	+0.1
4,775,015	-	DZA65_RS22085	0.55	-0.5
4,775,015	-	DZA65_RS22085	0.55	-1.0
4,775,015	-	DZA65_RS22085	0.55	-0.4
4,775,015	-	DZA65_RS22085	0.55	-0.9
4,775,015	-	DZA65_RS22085	0.55	-0.4
4,775,015	-	DZA65_RS22085	0.55	-0.8
4,775,015	-	DZA65_RS22085	0.55	+0.8
4,775,015	-	DZA65_RS22085	0.55	-0.9
4,775,015	-			+1.8
4,775,015	-	DZA65_RS22085	0.55	+0.0
4,775,015	-	DZA65_RS22085	0.55	+0.4
4,775,028	+	DZA65_RS22085	0.57	+0.2
4,775,035	+	DZA65_RS22085	0.57	-1.0
4,775,036	-	DZA65_RS22085	0.58	-0.2
4,775,036	-	DZA65_RS22085	0.58	-0.2
4,775,036	-	DZA65_RS22085	0.58	-2.6
4,775,036	-	DZA65_RS22085	0.58	+0.7
4,775,143	+	DZA65_RS22085	0.70	-0.9
4,775,143	+	DZA65_RS22085	0.70	-0.9
4,775,143	+	DZA65_RS22085	0.70	-1.0
4,775,143	+	DZA65_RS22085	0.70	+1.3
4,775,143	+	DZA65_RS22085	0.70	+0.3
4,775,143	+	DZA65_RS22085	0.70	-0.3
4,775,143	+	DZA65_RS22085	0.70	-1.6
4,775,143	+	DZA65_RS22085	0.70	-0.5
4,775,143	+	DZA65_RS22085	0.70	-0.4
4,775,144	-	DZA65_RS22085	0.70	-1.4
4,775,144	-	DZA65_RS22085	0.70	-1.1
4,775,144	-	DZA65_RS22085	0.70	-1.4
4,775,144	-	DZA65_RS22085	0.70	-1.4
4,775,144	-	DZA65_RS22085	0.70	-1.5
4,775,144	-	DZA65_RS22085	0.70	+0.8
4,775,151	+	DZA65_RS22085	0.70	-0.1
4,775,175	+	DZA65_RS22085	0.73	+0.0
4,775,175	+	DZA65_RS22085	0.73	-0.5
4,775,427	+			-0.2
4,775,427	+			-0.2
4,775,427	+			+1.1
4,775,553	+	DZA65_RS22090	0.16	-0.3
4,775,553	+	DZA65_RS22090	0.16	-0.5
4,775,553	+	DZA65_RS22090	0.16	-1.0
4,775,553	+	DZA65_RS22090	0.16	+0.5
4,775,554	-	DZA65_RS22090	0.16	-1.2
4,775,554	-	DZA65_RS22090	0.16	-0.4
4,775,554	-	DZA65_RS22090	0.16	-0.8
4,775,554	-	DZA65_RS22090	0.16	-0.4
4,775,554	-	DZA65_RS22090	0.16	+0.9
4,775,554	-	DZA65_RS22090	0.16	-0.4
4,775,554	-	DZA65_RS22090	0.16	+0.2
4,775,554	-	DZA65_RS22090	0.16	+0.4
4,775,554	-	DZA65_RS22090	0.16	-0.8
4,775,554	-	DZA65_RS22090	0.16	-0.5
4,775,642	+	DZA65_RS22090	0.26	-1.6
4,775,690	+	DZA65_RS22090	0.32	+0.1
4,775,690	+	DZA65_RS22090	0.32	-0.1
4,775,805	-	DZA65_RS22090	0.45	+1.8
4,775,985	+	DZA65_RS22090	0.66	-0.8
4,775,986	-	DZA65_RS22090	0.66	-0.2
4,776,045	-	DZA65_RS22090	0.73	-0.5
4,776,129	+	DZA65_RS22090	0.83	-0.5
4,776,133	+	DZA65_RS22090	0.84	-1.5
4,776,134	-	DZA65_RS22090	0.84	+0.4
4,776,153	+			-0.2
4,776,154	-	DZA65_RS22090	0.86	-2.0
4,776,166	+	DZA65_RS22090	0.88	-1.7
4,776,166	+	DZA65_RS22090	0.88	+0.1
4,776,166	+	DZA65_RS22090	0.88	+0.4
4,776,166	+	DZA65_RS22090	0.88	-1.3
4,776,166	+	DZA65_RS22090	0.88	-0.1
4,776,167	-	DZA65_RS22090	0.88	+0.5
4,776,167	-	DZA65_RS22090	0.88	-1.4
4,776,167	-	DZA65_RS22090	0.88	-0.8
4,776,168	+	DZA65_RS22090	0.88	+0.1
4,776,169	-	DZA65_RS22090	0.88	+0.5
4,776,169	-	DZA65_RS22090	0.88	-0.5
4,776,169	-	DZA65_RS22090	0.88	-1.0
4,776,169	-	DZA65_RS22090	0.88	-0.2
4,776,190	+			+0.5
4,776,190	+			+0.5
4,776,190	+			-0.1
4,776,191	-			+2.0
4,776,192	+			-0.6
4,776,192	+			-0.9
4,776,192	+			-1.0
4,776,193	-			-1.2
4,776,193	-			-0.5
4,776,193	-			+0.8
4,776,193	-			-1.4
4,776,193	-			-0.2
4,776,193	-			+0.2
4,776,253	+			-0.2
4,776,254	-			+0.2
4,776,254	-			-0.2
4,776,299	-			-1.8
4,776,302	+			-0.4
4,776,302	+			-2.1
4,776,303	-			+0.8
4,776,311	+			-0.4
4,776,316	+			-0.4
4,776,316	+			+0.2
4,776,353	+			+1.5
4,776,353	+			-0.8
4,776,353	+			-0.5
4,776,353	+			-0.4
4,776,353	+			-1.2
4,776,354	-			-0.5
4,776,354	-			+0.8
4,776,354	-			+0.5
4,776,354	-			-0.2
4,776,357	-			-1.2
4,776,357	-			-0.1
4,776,371	-			+1.0
4,776,371	-			-1.0
4,776,414	+			-0.5

Or see this region's nucleotide sequence