Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS19965 and DZA65_RS19970 are separated by 31 nucleotides DZA65_RS19970 and DZA65_RS19975 are separated by 459 nucleotides DZA65_RS19975 and DZA65_RS19980 overlap by 14 nucleotides
DZA65_RS19965: DZA65_RS19965 - cysteine desulfurase NifS, at 4,319,044 to 4,320,306
_RS19965
DZA65_RS19970: DZA65_RS19970 - Fe-S cluster assembly protein NifU, at 4,320,338 to 4,321,183
_RS19970
DZA65_RS19975: DZA65_RS19975 - nitrogen fixation protein NifX, at 4,321,643 to 4,322,110
_RS19975
DZA65_RS19980: DZA65_RS19980 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifN, at 4,322,097 to 4,323,482
_RS19980
Position (kb)
4320
4321
4322 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4319.361 kb on + strand, within DZA65_RS19965 at 4319.362 kb on - strand, within DZA65_RS19965 at 4319.362 kb on - strand, within DZA65_RS19965 at 4319.370 kb on + strand, within DZA65_RS19965 at 4319.370 kb on + strand, within DZA65_RS19965 at 4319.371 kb on - strand, within DZA65_RS19965 at 4319.488 kb on + strand, within DZA65_RS19965 at 4319.488 kb on + strand, within DZA65_RS19965 at 4319.488 kb on + strand, within DZA65_RS19965 at 4319.488 kb on + strand, within DZA65_RS19965 at 4319.489 kb on - strand, within DZA65_RS19965 at 4319.489 kb on - strand at 4319.535 kb on + strand, within DZA65_RS19965 at 4319.615 kb on + strand, within DZA65_RS19965 at 4319.615 kb on + strand, within DZA65_RS19965 at 4319.615 kb on + strand, within DZA65_RS19965 at 4319.615 kb on + strand, within DZA65_RS19965 at 4319.615 kb on + strand, within DZA65_RS19965 at 4319.615 kb on + strand, within DZA65_RS19965 at 4319.616 kb on - strand, within DZA65_RS19965 at 4319.616 kb on - strand, within DZA65_RS19965 at 4319.616 kb on - strand, within DZA65_RS19965 at 4319.740 kb on + strand, within DZA65_RS19965 at 4319.740 kb on + strand at 4319.740 kb on + strand, within DZA65_RS19965 at 4319.740 kb on + strand, within DZA65_RS19965 at 4319.740 kb on + strand, within DZA65_RS19965 at 4319.740 kb on + strand, within DZA65_RS19965 at 4319.740 kb on + strand, within DZA65_RS19965 at 4319.740 kb on + strand, within DZA65_RS19965 at 4319.740 kb on + strand, within DZA65_RS19965 at 4319.740 kb on + strand, within DZA65_RS19965 at 4319.741 kb on - strand, within DZA65_RS19965 at 4319.741 kb on - strand, within DZA65_RS19965 at 4319.741 kb on - strand, within DZA65_RS19965 at 4319.741 kb on - strand, within DZA65_RS19965 at 4319.741 kb on - strand, within DZA65_RS19965 at 4319.741 kb on - strand, within DZA65_RS19965 at 4319.741 kb on - strand, within DZA65_RS19965 at 4319.741 kb on - strand, within DZA65_RS19965 at 4319.741 kb on - strand, within DZA65_RS19965 at 4319.855 kb on + strand, within DZA65_RS19965 at 4319.856 kb on - strand, within DZA65_RS19965 at 4319.907 kb on + strand, within DZA65_RS19965 at 4319.907 kb on + strand, within DZA65_RS19965 at 4319.907 kb on + strand, within DZA65_RS19965 at 4319.907 kb on + strand, within DZA65_RS19965 at 4319.908 kb on - strand, within DZA65_RS19965 at 4319.909 kb on + strand, within DZA65_RS19965 at 4319.909 kb on + strand, within DZA65_RS19965 at 4319.909 kb on + strand, within DZA65_RS19965 at 4319.910 kb on - strand, within DZA65_RS19965 at 4319.910 kb on - strand, within DZA65_RS19965 at 4319.910 kb on - strand, within DZA65_RS19965 at 4320.048 kb on + strand, within DZA65_RS19965 at 4320.049 kb on - strand, within DZA65_RS19965 at 4320.068 kb on + strand, within DZA65_RS19965 at 4320.069 kb on - strand, within DZA65_RS19965 at 4320.294 kb on - strand at 4320.335 kb on + strand at 4320.336 kb on - strand at 4320.336 kb on - strand at 4320.339 kb on + strand at 4320.339 kb on + strand at 4320.339 kb on + strand at 4320.339 kb on + strand at 4320.339 kb on + strand at 4320.340 kb on - strand at 4320.340 kb on - strand at 4320.358 kb on + strand at 4320.358 kb on + strand at 4320.358 kb on + strand at 4320.358 kb on + strand at 4320.358 kb on + strand at 4320.358 kb on + strand at 4320.358 kb on + strand at 4320.359 kb on - strand at 4320.359 kb on - strand at 4320.359 kb on - strand at 4320.359 kb on - strand at 4320.359 kb on - strand at 4320.359 kb on - strand at 4320.359 kb on - strand at 4320.359 kb on - strand at 4320.360 kb on + strand at 4320.360 kb on + strand at 4320.360 kb on + strand at 4320.360 kb on + strand at 4320.360 kb on + strand at 4320.360 kb on + strand at 4320.360 kb on + strand at 4320.360 kb on + strand at 4320.360 kb on + strand at 4320.360 kb on + strand at 4320.360 kb on + strand at 4320.360 kb on + strand at 4320.360 kb on + strand at 4320.360 kb on + strand at 4320.361 kb on - strand at 4320.361 kb on - strand at 4320.361 kb on - strand at 4320.361 kb on - strand at 4320.361 kb on - strand at 4320.361 kb on - strand at 4320.361 kb on - strand at 4320.361 kb on - strand at 4320.361 kb on - strand at 4320.361 kb on - strand at 4320.361 kb on - strand at 4320.487 kb on + strand, within DZA65_RS19970 at 4320.487 kb on + strand, within DZA65_RS19970 at 4320.487 kb on + strand, within DZA65_RS19970 at 4320.487 kb on + strand, within DZA65_RS19970 at 4320.488 kb on - strand, within DZA65_RS19970 at 4320.488 kb on - strand, within DZA65_RS19970 at 4320.690 kb on + strand, within DZA65_RS19970 at 4320.690 kb on + strand, within DZA65_RS19970 at 4320.691 kb on - strand, within DZA65_RS19970 at 4320.772 kb on + strand, within DZA65_RS19970 at 4320.772 kb on + strand, within DZA65_RS19970 at 4320.773 kb on - strand, within DZA65_RS19970 at 4320.773 kb on - strand, within DZA65_RS19970 at 4320.773 kb on - strand, within DZA65_RS19970 at 4320.773 kb on - strand, within DZA65_RS19970 at 4320.822 kb on + strand, within DZA65_RS19970 at 4320.822 kb on + strand, within DZA65_RS19970 at 4320.822 kb on + strand, within DZA65_RS19970 at 4320.822 kb on + strand, within DZA65_RS19970 at 4320.823 kb on - strand, within DZA65_RS19970 at 4321.145 kb on - strand at 4321.173 kb on + strand at 4321.173 kb on + strand at 4321.173 kb on + strand at 4321.173 kb on + strand at 4321.173 kb on + strand at 4321.174 kb on - strand at 4321.174 kb on - strand at 4321.174 kb on - strand at 4321.174 kb on - strand at 4321.174 kb on - strand at 4321.198 kb on - strand at 4321.364 kb on + strand at 4321.365 kb on - strand at 4321.392 kb on + strand at 4321.392 kb on + strand at 4321.413 kb on + strand at 4321.413 kb on + strand at 4321.413 kb on + strand at 4321.413 kb on + strand at 4321.413 kb on + strand at 4321.413 kb on + strand at 4321.413 kb on + strand at 4321.413 kb on + strand at 4321.413 kb on + strand at 4321.413 kb on + strand at 4321.413 kb on + strand at 4321.413 kb on + strand at 4321.413 kb on + strand at 4321.413 kb on + strand at 4321.414 kb on - strand at 4321.414 kb on - strand at 4321.414 kb on - strand at 4321.414 kb on - strand at 4321.414 kb on - strand at 4321.414 kb on - strand at 4321.414 kb on - strand at 4321.414 kb on - strand at 4321.414 kb on - strand at 4321.414 kb on - strand at 4321.414 kb on - strand at 4321.414 kb on - strand at 4321.414 kb on - strand at 4321.414 kb on - strand at 4321.414 kb on - strand at 4321.414 kb on - strand at 4321.424 kb on - strand at 4321.491 kb on + strand at 4321.501 kb on + strand at 4321.502 kb on - strand at 4321.512 kb on + strand at 4321.557 kb on + strand at 4321.557 kb on + strand at 4321.557 kb on + strand at 4321.558 kb on - strand at 4321.558 kb on - strand at 4321.558 kb on - strand at 4321.558 kb on - strand at 4321.558 kb on - strand at 4321.558 kb on - strand at 4321.558 kb on - strand at 4321.597 kb on + strand at 4321.597 kb on + strand at 4321.597 kb on + strand at 4321.598 kb on - strand at 4321.598 kb on - strand at 4321.598 kb on - strand at 4321.796 kb on + strand, within DZA65_RS19975 at 4321.863 kb on + strand, within DZA65_RS19975 at 4321.863 kb on + strand, within DZA65_RS19975 at 4321.864 kb on - strand, within DZA65_RS19975 at 4321.976 kb on + strand, within DZA65_RS19975 at 4321.976 kb on + strand, within DZA65_RS19975 at 4321.977 kb on - strand, within DZA65_RS19975 at 4321.977 kb on - strand, within DZA65_RS19975 at 4321.983 kb on + strand, within DZA65_RS19975 at 4321.983 kb on + strand, within DZA65_RS19975 at 4321.983 kb on + strand, within DZA65_RS19975 at 4321.984 kb on - strand, within DZA65_RS19975 at 4321.984 kb on - strand, within DZA65_RS19975 at 4322.032 kb on - strand, within DZA65_RS19975 at 4322.165 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,319,361 + DZA65_RS19965 0.25 +0.4 4,319,362 - DZA65_RS19965 0.25 -0.7 4,319,362 - DZA65_RS19965 0.25 -0.1 4,319,370 + DZA65_RS19965 0.26 -0.6 4,319,370 + DZA65_RS19965 0.26 -1.0 4,319,371 - DZA65_RS19965 0.26 +0.8 4,319,488 + DZA65_RS19965 0.35 +1.0 4,319,488 + DZA65_RS19965 0.35 -0.5 4,319,488 + DZA65_RS19965 0.35 +1.5 4,319,488 + DZA65_RS19965 0.35 -0.6 4,319,489 - DZA65_RS19965 0.35 -0.6 4,319,489 - +0.1 4,319,535 + DZA65_RS19965 0.39 -0.6 4,319,615 + DZA65_RS19965 0.45 -1.6 4,319,615 + DZA65_RS19965 0.45 -0.3 4,319,615 + DZA65_RS19965 0.45 -0.2 4,319,615 + DZA65_RS19965 0.45 +0.5 4,319,615 + DZA65_RS19965 0.45 -1.5 4,319,615 + DZA65_RS19965 0.45 +0.5 4,319,616 - DZA65_RS19965 0.45 -0.2 4,319,616 - DZA65_RS19965 0.45 -1.5 4,319,616 - DZA65_RS19965 0.45 -0.9 4,319,740 + DZA65_RS19965 0.55 -0.6 4,319,740 + +0.7 4,319,740 + DZA65_RS19965 0.55 -2.4 4,319,740 + DZA65_RS19965 0.55 +0.8 4,319,740 + DZA65_RS19965 0.55 -0.2 4,319,740 + DZA65_RS19965 0.55 -1.6 4,319,740 + DZA65_RS19965 0.55 -0.3 4,319,740 + DZA65_RS19965 0.55 +0.2 4,319,740 + DZA65_RS19965 0.55 -0.6 4,319,740 + DZA65_RS19965 0.55 +1.0 4,319,741 - DZA65_RS19965 0.55 -1.3 4,319,741 - DZA65_RS19965 0.55 -1.1 4,319,741 - DZA65_RS19965 0.55 -0.1 4,319,741 - DZA65_RS19965 0.55 -1.6 4,319,741 - DZA65_RS19965 0.55 +0.3 4,319,741 - DZA65_RS19965 0.55 +0.3 4,319,741 - DZA65_RS19965 0.55 +0.3 4,319,741 - DZA65_RS19965 0.55 +0.5 4,319,741 - DZA65_RS19965 0.55 -0.9 4,319,855 + DZA65_RS19965 0.64 -1.8 4,319,856 - DZA65_RS19965 0.64 -0.5 4,319,907 + DZA65_RS19965 0.68 -1.9 4,319,907 + DZA65_RS19965 0.68 -0.8 4,319,907 + DZA65_RS19965 0.68 +0.5 4,319,907 + DZA65_RS19965 0.68 +0.3 4,319,908 - DZA65_RS19965 0.68 -1.0 4,319,909 + DZA65_RS19965 0.68 +0.3 4,319,909 + DZA65_RS19965 0.68 +0.3 4,319,909 + DZA65_RS19965 0.68 +0.1 4,319,910 - DZA65_RS19965 0.69 -0.1 4,319,910 - DZA65_RS19965 0.69 -1.3 4,319,910 - DZA65_RS19965 0.69 -0.2 4,320,048 + DZA65_RS19965 0.79 -2.2 4,320,049 - DZA65_RS19965 0.80 -1.5 4,320,068 + DZA65_RS19965 0.81 -0.6 4,320,069 - DZA65_RS19965 0.81 -0.5 4,320,294 - -0.4 4,320,335 + -0.3 4,320,336 - +0.7 4,320,336 - -0.4 4,320,339 + -1.5 4,320,339 + -0.3 4,320,339 + -0.6 4,320,339 + -0.3 4,320,339 + +0.1 4,320,340 - +1.6 4,320,340 - -0.9 4,320,358 + +0.5 4,320,358 + +1.7 4,320,358 + -0.3 4,320,358 + -1.5 4,320,358 + -0.2 4,320,358 + -0.4 4,320,358 + +1.0 4,320,359 - -0.4 4,320,359 - -0.3 4,320,359 - +0.7 4,320,359 - +1.7 4,320,359 - -0.1 4,320,359 - -0.8 4,320,359 - -0.7 4,320,359 - +2.3 4,320,360 + -0.3 4,320,360 + -1.3 4,320,360 + -1.1 4,320,360 + +0.1 4,320,360 + -0.5 4,320,360 + +0.3 4,320,360 + -0.6 4,320,360 + +0.1 4,320,360 + -0.6 4,320,360 + +0.3 4,320,360 + +0.6 4,320,360 + -0.1 4,320,360 + -0.2 4,320,360 + -0.8 4,320,361 - -0.6 4,320,361 - +0.2 4,320,361 - +1.7 4,320,361 - -2.2 4,320,361 - -0.1 4,320,361 - +0.1 4,320,361 - -1.0 4,320,361 - -0.2 4,320,361 - -1.3 4,320,361 - -1.0 4,320,361 - +0.4 4,320,487 + DZA65_RS19970 0.18 -1.5 4,320,487 + DZA65_RS19970 0.18 -1.1 4,320,487 + DZA65_RS19970 0.18 -1.8 4,320,487 + DZA65_RS19970 0.18 -0.0 4,320,488 - DZA65_RS19970 0.18 +0.4 4,320,488 - DZA65_RS19970 0.18 -0.8 4,320,690 + DZA65_RS19970 0.42 -1.2 4,320,690 + DZA65_RS19970 0.42 +0.1 4,320,691 - DZA65_RS19970 0.42 -0.5 4,320,772 + DZA65_RS19970 0.51 -2.2 4,320,772 + DZA65_RS19970 0.51 +0.9 4,320,773 - DZA65_RS19970 0.51 -0.5 4,320,773 - DZA65_RS19970 0.51 -0.3 4,320,773 - DZA65_RS19970 0.51 +0.3 4,320,773 - DZA65_RS19970 0.51 +0.1 4,320,822 + DZA65_RS19970 0.57 -0.6 4,320,822 + DZA65_RS19970 0.57 +0.5 4,320,822 + DZA65_RS19970 0.57 -0.1 4,320,822 + DZA65_RS19970 0.57 -1.3 4,320,823 - DZA65_RS19970 0.57 -2.0 4,321,145 - +0.7 4,321,173 + +0.7 4,321,173 + -0.3 4,321,173 + -0.9 4,321,173 + -0.9 4,321,173 + +0.7 4,321,174 - -1.6 4,321,174 - -1.6 4,321,174 - -0.5 4,321,174 - +0.9 4,321,174 - -0.9 4,321,198 - -0.3 4,321,364 + -0.6 4,321,365 - -1.1 4,321,392 + -0.8 4,321,392 + -1.5 4,321,413 + -1.8 4,321,413 + -1.1 4,321,413 + +0.7 4,321,413 + -0.3 4,321,413 + -0.4 4,321,413 + +0.5 4,321,413 + +0.9 4,321,413 + -0.2 4,321,413 + -2.5 4,321,413 + -1.5 4,321,413 + -0.2 4,321,413 + -1.5 4,321,413 + +0.3 4,321,413 + -0.0 4,321,414 - -0.8 4,321,414 - -1.3 4,321,414 - +1.7 4,321,414 - -0.3 4,321,414 - +0.3 4,321,414 - +2.0 4,321,414 - +0.4 4,321,414 - -1.3 4,321,414 - -1.1 4,321,414 - -0.1 4,321,414 - -1.0 4,321,414 - -1.0 4,321,414 - -1.8 4,321,414 - -0.6 4,321,414 - -0.0 4,321,414 - +0.9 4,321,424 - -0.8 4,321,491 + -0.4 4,321,501 + -0.7 4,321,502 - -0.6 4,321,512 + -0.2 4,321,557 + -0.6 4,321,557 + -0.1 4,321,557 + +0.9 4,321,558 - -1.3 4,321,558 - -1.3 4,321,558 - +0.1 4,321,558 - +2.0 4,321,558 - -1.0 4,321,558 - +0.1 4,321,558 - -0.3 4,321,597 + -0.9 4,321,597 + -0.8 4,321,597 + -0.8 4,321,598 - +0.6 4,321,598 - +0.4 4,321,598 - -1.7 4,321,796 + DZA65_RS19975 0.33 +0.9 4,321,863 + DZA65_RS19975 0.47 -0.6 4,321,863 + DZA65_RS19975 0.47 -0.6 4,321,864 - DZA65_RS19975 0.47 +0.3 4,321,976 + DZA65_RS19975 0.71 -1.3 4,321,976 + DZA65_RS19975 0.71 -0.3 4,321,977 - DZA65_RS19975 0.71 -0.3 4,321,977 - DZA65_RS19975 0.71 -0.3 4,321,983 + DZA65_RS19975 0.73 +0.5 4,321,983 + DZA65_RS19975 0.73 -0.3 4,321,983 + DZA65_RS19975 0.73 -0.3 4,321,984 - DZA65_RS19975 0.73 -0.4 4,321,984 - DZA65_RS19975 0.73 -0.9 4,322,032 - DZA65_RS19975 0.83 +0.9 4,322,165 - +0.0
Or see this region's nucleotide sequence