Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS19080 and DZA65_RS19085 are separated by 97 nucleotides DZA65_RS19085 and DZA65_RS19090 are separated by 27 nucleotides DZA65_RS19090 and DZA65_RS19095 are separated by 16 nucleotides
DZA65_RS19080: DZA65_RS19080 - preprotein translocase subunit SecA, at 4,123,271 to 4,125,964
_RS19080
DZA65_RS19085: DZA65_RS19085 - secA regulator SecM, at 4,126,062 to 4,126,562
_RS19085
DZA65_RS19090: DZA65_RS19090 - DUF721 domain-containing protein, at 4,126,590 to 4,127,111
_RS19090
DZA65_RS19095: DZA65_RS19095 - UDP-3-O-acyl-N-acetylglucosamine deacetylase, at 4,127,128 to 4,128,045
_RS19095
Position (kb)
4126
4127
4128 Strain fitness (log2 ratio)
-2
-1
0
1 at 4126.228 kb on - strand, within DZA65_RS19085 at 4126.391 kb on - strand at 4126.391 kb on - strand, within DZA65_RS19085 at 4126.940 kb on + strand, within DZA65_RS19090 at 4126.940 kb on + strand, within DZA65_RS19090 at 4126.940 kb on + strand, within DZA65_RS19090 at 4126.958 kb on + strand, within DZA65_RS19090 at 4126.958 kb on + strand, within DZA65_RS19090 at 4126.959 kb on - strand, within DZA65_RS19090 at 4126.991 kb on + strand, within DZA65_RS19090 at 4127.009 kb on - strand, within DZA65_RS19090 at 4127.053 kb on + strand, within DZA65_RS19090 at 4127.056 kb on + strand, within DZA65_RS19090 at 4127.087 kb on + strand at 4127.087 kb on + strand at 4127.088 kb on - strand at 4127.109 kb on + strand at 4127.109 kb on + strand at 4127.110 kb on - strand at 4127.110 kb on - strand at 4127.143 kb on - strand at 4128.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,126,228 - DZA65_RS19085 0.33 -0.9 4,126,391 - +1.7 4,126,391 - DZA65_RS19085 0.66 -1.9 4,126,940 + DZA65_RS19090 0.67 -2.4 4,126,940 + DZA65_RS19090 0.67 -0.7 4,126,940 + DZA65_RS19090 0.67 +0.1 4,126,958 + DZA65_RS19090 0.70 -1.0 4,126,958 + DZA65_RS19090 0.70 -1.5 4,126,959 - DZA65_RS19090 0.71 -1.3 4,126,991 + DZA65_RS19090 0.77 -0.3 4,127,009 - DZA65_RS19090 0.80 -0.2 4,127,053 + DZA65_RS19090 0.89 +1.3 4,127,056 + DZA65_RS19090 0.89 +1.1 4,127,087 + -0.5 4,127,087 + -0.9 4,127,088 - -2.0 4,127,109 + -0.2 4,127,109 + -0.4 4,127,110 - -1.0 4,127,110 - -1.8 4,127,143 - +0.3 4,128,073 - -0.1
Or see this region's nucleotide sequence