Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS13570 and DZA65_RS13575 are separated by 360 nucleotides DZA65_RS13575 and DZA65_RS13580 are separated by 294 nucleotides DZA65_RS13580 and DZA65_RS13585 are separated by 35 nucleotides
DZA65_RS13570: DZA65_RS13570 - glycine zipper 2TM domain-containing protein, at 2,915,058 to 2,915,597
_RS13570
DZA65_RS13575: DZA65_RS13575 - NAD(P)/FAD-dependent oxidoreductase, at 2,915,958 to 2,917,265
_RS13575
DZA65_RS13580: DZA65_RS13580 - alpha/beta hydrolase, at 2,917,560 to 2,918,102
_RS13580
DZA65_RS13585: DZA65_RS13585 - beta-N-acetylhexosaminidase, at 2,918,138 to 2,919,163
_RS13585
Position (kb)
2915
2916
2917
2918 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2914.962 kb on + strand at 2914.962 kb on + strand at 2914.962 kb on + strand at 2914.962 kb on + strand at 2914.962 kb on + strand at 2914.963 kb on - strand at 2915.159 kb on + strand, within DZA65_RS13570 at 2915.159 kb on + strand, within DZA65_RS13570 at 2915.160 kb on - strand, within DZA65_RS13570 at 2915.161 kb on + strand, within DZA65_RS13570 at 2915.162 kb on - strand, within DZA65_RS13570 at 2915.201 kb on - strand, within DZA65_RS13570 at 2915.278 kb on + strand, within DZA65_RS13570 at 2915.310 kb on + strand, within DZA65_RS13570 at 2915.311 kb on - strand, within DZA65_RS13570 at 2915.311 kb on - strand, within DZA65_RS13570 at 2915.311 kb on - strand, within DZA65_RS13570 at 2915.472 kb on - strand, within DZA65_RS13570 at 2915.562 kb on - strand at 2915.613 kb on + strand at 2915.614 kb on - strand at 2915.614 kb on - strand at 2915.617 kb on + strand at 2915.618 kb on - strand at 2915.647 kb on + strand at 2915.647 kb on + strand at 2915.648 kb on - strand at 2915.648 kb on - strand at 2915.648 kb on - strand at 2915.650 kb on + strand at 2915.650 kb on + strand at 2915.651 kb on - strand at 2915.667 kb on + strand at 2915.668 kb on - strand at 2915.668 kb on - strand at 2915.668 kb on - strand at 2915.668 kb on - strand at 2915.686 kb on - strand at 2915.731 kb on - strand at 2915.771 kb on - strand at 2915.786 kb on - strand at 2915.786 kb on - strand at 2915.810 kb on + strand at 2915.847 kb on - strand at 2915.859 kb on + strand at 2915.859 kb on + strand at 2915.860 kb on - strand at 2915.864 kb on + strand at 2915.864 kb on + strand at 2915.890 kb on + strand at 2916.056 kb on + strand at 2916.056 kb on + strand at 2916.056 kb on + strand at 2916.056 kb on + strand at 2916.057 kb on - strand at 2916.057 kb on - strand at 2916.074 kb on - strand at 2916.187 kb on + strand, within DZA65_RS13575 at 2916.187 kb on + strand, within DZA65_RS13575 at 2916.193 kb on + strand, within DZA65_RS13575 at 2916.193 kb on + strand, within DZA65_RS13575 at 2916.194 kb on - strand, within DZA65_RS13575 at 2916.213 kb on - strand, within DZA65_RS13575 at 2916.230 kb on + strand, within DZA65_RS13575 at 2916.231 kb on - strand, within DZA65_RS13575 at 2916.231 kb on - strand, within DZA65_RS13575 at 2916.231 kb on - strand at 2916.239 kb on - strand, within DZA65_RS13575 at 2916.411 kb on - strand, within DZA65_RS13575 at 2916.489 kb on - strand, within DZA65_RS13575 at 2916.552 kb on - strand, within DZA65_RS13575 at 2916.606 kb on - strand, within DZA65_RS13575 at 2916.606 kb on - strand, within DZA65_RS13575 at 2916.746 kb on + strand, within DZA65_RS13575 at 2916.861 kb on - strand, within DZA65_RS13575 at 2917.060 kb on + strand, within DZA65_RS13575 at 2917.060 kb on + strand, within DZA65_RS13575 at 2917.061 kb on - strand, within DZA65_RS13575 at 2917.061 kb on - strand, within DZA65_RS13575 at 2917.155 kb on + strand at 2917.155 kb on + strand at 2917.326 kb on + strand at 2917.329 kb on + strand at 2917.359 kb on - strand at 2917.411 kb on - strand at 2917.413 kb on - strand at 2917.420 kb on + strand at 2917.429 kb on + strand at 2917.444 kb on + strand at 2917.453 kb on - strand at 2917.574 kb on + strand at 2917.575 kb on - strand at 2917.608 kb on - strand at 2917.619 kb on + strand, within DZA65_RS13580 at 2917.619 kb on + strand, within DZA65_RS13580 at 2917.651 kb on + strand, within DZA65_RS13580 at 2917.654 kb on + strand, within DZA65_RS13580 at 2917.654 kb on + strand, within DZA65_RS13580 at 2917.654 kb on + strand, within DZA65_RS13580 at 2917.655 kb on - strand, within DZA65_RS13580 at 2917.721 kb on + strand, within DZA65_RS13580 at 2917.722 kb on - strand, within DZA65_RS13580 at 2917.722 kb on - strand, within DZA65_RS13580 at 2917.722 kb on - strand, within DZA65_RS13580 at 2917.722 kb on - strand, within DZA65_RS13580 at 2917.780 kb on + strand, within DZA65_RS13580 at 2917.780 kb on + strand, within DZA65_RS13580 at 2917.780 kb on + strand, within DZA65_RS13580 at 2917.780 kb on + strand, within DZA65_RS13580 at 2917.781 kb on - strand, within DZA65_RS13580 at 2917.812 kb on - strand, within DZA65_RS13580 at 2917.812 kb on - strand, within DZA65_RS13580 at 2917.845 kb on + strand, within DZA65_RS13580 at 2917.845 kb on + strand, within DZA65_RS13580 at 2917.845 kb on + strand, within DZA65_RS13580 at 2917.845 kb on + strand, within DZA65_RS13580 at 2917.846 kb on - strand, within DZA65_RS13580 at 2917.846 kb on - strand, within DZA65_RS13580 at 2918.002 kb on + strand, within DZA65_RS13580 at 2918.002 kb on + strand, within DZA65_RS13580 at 2918.002 kb on + strand, within DZA65_RS13580 at 2918.002 kb on + strand, within DZA65_RS13580 at 2918.002 kb on + strand, within DZA65_RS13580 at 2918.003 kb on - strand, within DZA65_RS13580 at 2918.003 kb on - strand, within DZA65_RS13580 at 2918.003 kb on - strand, within DZA65_RS13580 at 2918.047 kb on - strand, within DZA65_RS13580 at 2918.060 kb on + strand at 2918.092 kb on + strand at 2918.092 kb on + strand at 2918.092 kb on + strand at 2918.093 kb on - strand at 2918.139 kb on + strand at 2918.253 kb on + strand, within DZA65_RS13585 at 2918.254 kb on - strand, within DZA65_RS13585 at 2918.254 kb on - strand, within DZA65_RS13585 at 2918.254 kb on - strand, within DZA65_RS13585
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,914,962 + +0.8 2,914,962 + -0.5 2,914,962 + +2.0 2,914,962 + +1.4 2,914,962 + -0.6 2,914,963 - -0.4 2,915,159 + DZA65_RS13570 0.19 -0.9 2,915,159 + DZA65_RS13570 0.19 +1.1 2,915,160 - DZA65_RS13570 0.19 +2.0 2,915,161 + DZA65_RS13570 0.19 -0.4 2,915,162 - DZA65_RS13570 0.19 +0.9 2,915,201 - DZA65_RS13570 0.26 -0.4 2,915,278 + DZA65_RS13570 0.41 -0.5 2,915,310 + DZA65_RS13570 0.47 -0.6 2,915,311 - DZA65_RS13570 0.47 +1.1 2,915,311 - DZA65_RS13570 0.47 -1.4 2,915,311 - DZA65_RS13570 0.47 -0.5 2,915,472 - DZA65_RS13570 0.77 -0.6 2,915,562 - -0.2 2,915,613 + -0.2 2,915,614 - -1.2 2,915,614 - +0.8 2,915,617 + -0.0 2,915,618 - -0.5 2,915,647 + -0.2 2,915,647 + -0.6 2,915,648 - +0.0 2,915,648 - -1.2 2,915,648 - +0.2 2,915,650 + -0.6 2,915,650 + +0.4 2,915,651 - +0.0 2,915,667 + -2.2 2,915,668 - +0.4 2,915,668 - +0.6 2,915,668 - -0.6 2,915,668 - -2.8 2,915,686 - -1.2 2,915,731 - -1.2 2,915,771 - +0.1 2,915,786 - -0.4 2,915,786 - -1.4 2,915,810 + +0.6 2,915,847 - -0.3 2,915,859 + -0.8 2,915,859 + -0.7 2,915,860 - -0.2 2,915,864 + -0.7 2,915,864 + -0.7 2,915,890 + +1.4 2,916,056 + -1.5 2,916,056 + -1.2 2,916,056 + -0.6 2,916,056 + -0.1 2,916,057 - -0.8 2,916,057 - -1.5 2,916,074 - +1.4 2,916,187 + DZA65_RS13575 0.18 -0.6 2,916,187 + DZA65_RS13575 0.18 -0.7 2,916,193 + DZA65_RS13575 0.18 -0.9 2,916,193 + DZA65_RS13575 0.18 -0.1 2,916,194 - DZA65_RS13575 0.18 -1.2 2,916,213 - DZA65_RS13575 0.19 -1.2 2,916,230 + DZA65_RS13575 0.21 +0.2 2,916,231 - DZA65_RS13575 0.21 -0.0 2,916,231 - DZA65_RS13575 0.21 -0.2 2,916,231 - +0.4 2,916,239 - DZA65_RS13575 0.21 -0.2 2,916,411 - DZA65_RS13575 0.35 -0.2 2,916,489 - DZA65_RS13575 0.41 +0.6 2,916,552 - DZA65_RS13575 0.45 -0.4 2,916,606 - DZA65_RS13575 0.50 -0.2 2,916,606 - DZA65_RS13575 0.50 -1.2 2,916,746 + DZA65_RS13575 0.60 -1.2 2,916,861 - DZA65_RS13575 0.69 +1.0 2,917,060 + DZA65_RS13575 0.84 -1.6 2,917,060 + DZA65_RS13575 0.84 -0.2 2,917,061 - DZA65_RS13575 0.84 +0.6 2,917,061 - DZA65_RS13575 0.84 -0.6 2,917,155 + +0.4 2,917,155 + -0.5 2,917,326 + +1.4 2,917,329 + -1.4 2,917,359 - -0.0 2,917,411 - -0.8 2,917,413 - -1.3 2,917,420 + -0.6 2,917,429 + -1.1 2,917,444 + +0.4 2,917,453 - +0.3 2,917,574 + +0.1 2,917,575 - -0.2 2,917,608 - +0.6 2,917,619 + DZA65_RS13580 0.11 +0.4 2,917,619 + DZA65_RS13580 0.11 -0.8 2,917,651 + DZA65_RS13580 0.17 +0.3 2,917,654 + DZA65_RS13580 0.17 -0.8 2,917,654 + DZA65_RS13580 0.17 +0.5 2,917,654 + DZA65_RS13580 0.17 +1.0 2,917,655 - DZA65_RS13580 0.17 +0.1 2,917,721 + DZA65_RS13580 0.30 -0.5 2,917,722 - DZA65_RS13580 0.30 -0.7 2,917,722 - DZA65_RS13580 0.30 -1.0 2,917,722 - DZA65_RS13580 0.30 -3.0 2,917,722 - DZA65_RS13580 0.30 +0.6 2,917,780 + DZA65_RS13580 0.41 -1.9 2,917,780 + DZA65_RS13580 0.41 +0.4 2,917,780 + DZA65_RS13580 0.41 -1.4 2,917,780 + DZA65_RS13580 0.41 +0.2 2,917,781 - DZA65_RS13580 0.41 -2.5 2,917,812 - DZA65_RS13580 0.46 -2.9 2,917,812 - DZA65_RS13580 0.46 -0.2 2,917,845 + DZA65_RS13580 0.52 -0.5 2,917,845 + DZA65_RS13580 0.52 +0.4 2,917,845 + DZA65_RS13580 0.52 +0.1 2,917,845 + DZA65_RS13580 0.52 +2.1 2,917,846 - DZA65_RS13580 0.53 -0.1 2,917,846 - DZA65_RS13580 0.53 -0.2 2,918,002 + DZA65_RS13580 0.81 -1.4 2,918,002 + DZA65_RS13580 0.81 -0.7 2,918,002 + DZA65_RS13580 0.81 -0.6 2,918,002 + DZA65_RS13580 0.81 +2.2 2,918,002 + DZA65_RS13580 0.81 -0.0 2,918,003 - DZA65_RS13580 0.82 +1.4 2,918,003 - DZA65_RS13580 0.82 +1.1 2,918,003 - DZA65_RS13580 0.82 -0.0 2,918,047 - DZA65_RS13580 0.90 -0.4 2,918,060 + -0.8 2,918,092 + -1.6 2,918,092 + -0.9 2,918,092 + -1.6 2,918,093 - -0.6 2,918,139 + -1.4 2,918,253 + DZA65_RS13585 0.11 +0.2 2,918,254 - DZA65_RS13585 0.11 +0.4 2,918,254 - DZA65_RS13585 0.11 +0.4 2,918,254 - DZA65_RS13585 0.11 -0.1
Or see this region's nucleotide sequence