Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS11405 and DZA65_RS11410 are separated by 209 nucleotides DZA65_RS11410 and DZA65_RS11415 are separated by 3 nucleotides DZA65_RS11415 and DZA65_RS11420 are separated by 129 nucleotides
DZA65_RS11405: DZA65_RS11405 - glutamyl-tRNA reductase, at 2,426,569 to 2,427,825
_RS11405
DZA65_RS11410: DZA65_RS11410 - lipoprotein localization protein LolB, at 2,428,035 to 2,428,655
_RS11410
DZA65_RS11415: DZA65_RS11415 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 2,428,659 to 2,429,531
_RS11415
DZA65_RS11420: DZA65_RS11420 - ribose-phosphate diphosphokinase, at 2,429,661 to 2,430,608
_RS11420
Position (kb)
2428
2429
2430 Strain fitness (log2 ratio)
-2
-1
0
1 at 2427.850 kb on - strand at 2427.850 kb on - strand at 2427.853 kb on - strand at 2427.853 kb on - strand at 2427.878 kb on - strand at 2427.878 kb on - strand at 2428.017 kb on + strand at 2429.529 kb on + strand at 2429.529 kb on + strand at 2429.529 kb on + strand at 2429.557 kb on + strand at 2429.580 kb on + strand at 2429.580 kb on + strand at 2429.584 kb on + strand at 2429.584 kb on + strand at 2429.584 kb on + strand at 2429.584 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,427,850 - -0.7 2,427,850 - -2.0 2,427,853 - -0.3 2,427,853 - +0.3 2,427,878 - -1.3 2,427,878 - +1.3 2,428,017 + -0.5 2,429,529 + -0.5 2,429,529 + +0.2 2,429,529 + -0.5 2,429,557 + -2.1 2,429,580 + -0.8 2,429,580 + -1.5 2,429,584 + -1.5 2,429,584 + -0.1 2,429,584 + -1.9 2,429,584 + -0.0
Or see this region's nucleotide sequence