Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS00010 and DZA65_RS00015 are separated by 165 nucleotides DZA65_RS00015 and DZA65_RS00020 are separated by 17 nucleotides
DZA65_RS00010: DZA65_RS00010 - DNA polymerase III subunit beta, at 1,325 to 2,425
_RS00010
DZA65_RS00015: DZA65_RS00015 - DNA replication/repair protein RecF, at 2,591 to 3,676
_RS00015
DZA65_RS00020: DZA65_RS00020 - DNA topoisomerase (ATP-hydrolyzing) subunit B, at 3,694 to 6,105
_RS00020
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2.423 kb on + strand at 2.424 kb on - strand at 2.517 kb on + strand at 2.517 kb on + strand at 2.517 kb on + strand at 2.517 kb on + strand at 2.518 kb on - strand at 2.518 kb on - strand at 2.531 kb on + strand at 2.531 kb on + strand at 2.531 kb on + strand at 2.532 kb on - strand at 2.532 kb on - strand at 2.532 kb on - strand at 2.532 kb on - strand at 2.540 kb on + strand at 2.540 kb on + strand at 2.611 kb on + strand at 2.612 kb on - strand at 2.630 kb on - strand at 2.720 kb on + strand, within DZA65_RS00015 at 2.720 kb on + strand, within DZA65_RS00015 at 2.720 kb on + strand, within DZA65_RS00015 at 2.720 kb on + strand, within DZA65_RS00015 at 2.720 kb on + strand, within DZA65_RS00015 at 2.720 kb on + strand, within DZA65_RS00015 at 2.720 kb on + strand, within DZA65_RS00015 at 2.720 kb on + strand, within DZA65_RS00015 at 2.721 kb on - strand, within DZA65_RS00015 at 2.721 kb on - strand, within DZA65_RS00015 at 2.721 kb on - strand, within DZA65_RS00015 at 2.721 kb on - strand, within DZA65_RS00015 at 2.721 kb on - strand, within DZA65_RS00015 at 2.721 kb on - strand, within DZA65_RS00015 at 2.721 kb on - strand, within DZA65_RS00015 at 2.721 kb on - strand, within DZA65_RS00015 at 2.721 kb on - strand at 2.721 kb on - strand, within DZA65_RS00015 at 2.721 kb on - strand, within DZA65_RS00015 at 2.721 kb on - strand, within DZA65_RS00015 at 2.721 kb on - strand, within DZA65_RS00015 at 2.721 kb on - strand, within DZA65_RS00015 at 2.752 kb on + strand, within DZA65_RS00015 at 2.753 kb on - strand, within DZA65_RS00015 at 2.810 kb on - strand, within DZA65_RS00015 at 2.930 kb on - strand, within DZA65_RS00015 at 2.982 kb on - strand, within DZA65_RS00015 at 3.047 kb on - strand, within DZA65_RS00015 at 3.047 kb on - strand, within DZA65_RS00015 at 3.073 kb on + strand, within DZA65_RS00015 at 3.073 kb on + strand, within DZA65_RS00015 at 3.073 kb on + strand, within DZA65_RS00015 at 3.101 kb on + strand, within DZA65_RS00015 at 3.102 kb on - strand, within DZA65_RS00015 at 3.173 kb on + strand, within DZA65_RS00015 at 3.173 kb on + strand, within DZA65_RS00015 at 3.173 kb on + strand, within DZA65_RS00015 at 3.174 kb on - strand, within DZA65_RS00015 at 3.174 kb on - strand, within DZA65_RS00015 at 3.278 kb on + strand at 3.279 kb on - strand, within DZA65_RS00015 at 3.485 kb on + strand, within DZA65_RS00015 at 3.485 kb on + strand, within DZA65_RS00015 at 3.602 kb on - strand at 3.649 kb on + strand at 3.674 kb on + strand at 3.674 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,423 + +0.4 2,424 - -2.0 2,517 + -1.1 2,517 + -0.7 2,517 + -0.2 2,517 + -1.6 2,518 - -0.5 2,518 - -0.1 2,531 + -0.1 2,531 + -1.2 2,531 + +0.1 2,532 - -0.0 2,532 - -1.3 2,532 - +0.8 2,532 - -0.7 2,540 + +0.6 2,540 + -0.9 2,611 + -1.0 2,612 - -1.7 2,630 - -0.6 2,720 + DZA65_RS00015 0.12 +0.5 2,720 + DZA65_RS00015 0.12 -1.4 2,720 + DZA65_RS00015 0.12 -1.6 2,720 + DZA65_RS00015 0.12 -1.1 2,720 + DZA65_RS00015 0.12 -0.6 2,720 + DZA65_RS00015 0.12 -0.6 2,720 + DZA65_RS00015 0.12 -0.4 2,720 + DZA65_RS00015 0.12 +0.9 2,721 - DZA65_RS00015 0.12 +0.4 2,721 - DZA65_RS00015 0.12 -0.6 2,721 - DZA65_RS00015 0.12 -1.0 2,721 - DZA65_RS00015 0.12 -0.5 2,721 - DZA65_RS00015 0.12 -0.0 2,721 - DZA65_RS00015 0.12 -1.0 2,721 - DZA65_RS00015 0.12 -0.6 2,721 - DZA65_RS00015 0.12 -0.5 2,721 - +1.8 2,721 - DZA65_RS00015 0.12 +0.4 2,721 - DZA65_RS00015 0.12 -0.7 2,721 - DZA65_RS00015 0.12 -1.3 2,721 - DZA65_RS00015 0.12 -2.4 2,721 - DZA65_RS00015 0.12 +0.8 2,752 + DZA65_RS00015 0.15 -1.0 2,753 - DZA65_RS00015 0.15 -2.6 2,810 - DZA65_RS00015 0.20 -0.8 2,930 - DZA65_RS00015 0.31 +0.5 2,982 - DZA65_RS00015 0.36 -1.2 3,047 - DZA65_RS00015 0.42 -0.8 3,047 - DZA65_RS00015 0.42 +0.2 3,073 + DZA65_RS00015 0.44 -0.1 3,073 + DZA65_RS00015 0.44 -2.5 3,073 + DZA65_RS00015 0.44 +1.3 3,101 + DZA65_RS00015 0.47 -1.0 3,102 - DZA65_RS00015 0.47 -0.2 3,173 + DZA65_RS00015 0.54 -1.0 3,173 + DZA65_RS00015 0.54 +0.2 3,173 + DZA65_RS00015 0.54 +0.0 3,174 - DZA65_RS00015 0.54 -0.8 3,174 - DZA65_RS00015 0.54 -0.4 3,278 + +0.2 3,279 - DZA65_RS00015 0.63 -0.0 3,485 + DZA65_RS00015 0.82 +0.2 3,485 + DZA65_RS00015 0.82 -0.9 3,602 - +1.3 3,649 + -0.8 3,674 + +2.2 3,674 + +0.8
Or see this region's nucleotide sequence