Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS05030 and DDA3937_RS05035 overlap by 13 nucleotides DDA3937_RS05035 and DDA3937_RS05040 are separated by 3 nucleotides
DDA3937_RS05030: DDA3937_RS05030 - tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA, at 1,125,566 to 1,126,399
_RS05030
DDA3937_RS05035: DDA3937_RS05035 - cysteine desulfurase sulfur acceptor subunit CsdE, at 1,126,387 to 1,126,830
_RS05035
DDA3937_RS05040: DDA3937_RS05040 - cysteine desulfurase CsdA, at 1,126,834 to 1,128,039
_RS05040
Position (kb)
1126
1127 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1125.390 kb on - strand at 1125.432 kb on + strand at 1125.433 kb on - strand at 1125.436 kb on + strand at 1125.436 kb on + strand at 1125.451 kb on - strand at 1125.467 kb on + strand at 1125.467 kb on + strand at 1125.467 kb on + strand at 1125.467 kb on + strand at 1125.467 kb on + strand at 1125.468 kb on - strand at 1125.468 kb on - strand at 1125.468 kb on - strand at 1125.468 kb on - strand at 1125.468 kb on - strand at 1125.468 kb on - strand at 1125.468 kb on - strand at 1125.528 kb on + strand at 1125.571 kb on + strand at 1125.623 kb on + strand at 1125.736 kb on + strand, within DDA3937_RS05030 at 1125.772 kb on + strand, within DDA3937_RS05030 at 1125.773 kb on - strand, within DDA3937_RS05030 at 1125.901 kb on + strand, within DDA3937_RS05030 at 1125.901 kb on + strand, within DDA3937_RS05030 at 1125.902 kb on - strand, within DDA3937_RS05030 at 1126.082 kb on - strand, within DDA3937_RS05030 at 1126.082 kb on - strand, within DDA3937_RS05030 at 1126.132 kb on + strand, within DDA3937_RS05030 at 1126.132 kb on + strand, within DDA3937_RS05030 at 1126.132 kb on + strand, within DDA3937_RS05030 at 1126.142 kb on - strand, within DDA3937_RS05030 at 1126.200 kb on - strand, within DDA3937_RS05030 at 1126.200 kb on - strand, within DDA3937_RS05030 at 1126.200 kb on - strand, within DDA3937_RS05030 at 1126.235 kb on - strand, within DDA3937_RS05030 at 1126.251 kb on + strand, within DDA3937_RS05030 at 1126.258 kb on + strand, within DDA3937_RS05030 at 1126.258 kb on + strand, within DDA3937_RS05030 at 1126.258 kb on + strand, within DDA3937_RS05030 at 1126.259 kb on - strand, within DDA3937_RS05030 at 1126.259 kb on - strand, within DDA3937_RS05030 at 1126.490 kb on - strand, within DDA3937_RS05035 at 1126.537 kb on + strand, within DDA3937_RS05035 at 1126.537 kb on + strand at 1126.538 kb on - strand, within DDA3937_RS05035 at 1126.719 kb on + strand, within DDA3937_RS05035 at 1126.720 kb on - strand, within DDA3937_RS05035 at 1126.847 kb on - strand at 1126.904 kb on + strand at 1126.904 kb on + strand at 1126.904 kb on + strand at 1126.905 kb on - strand at 1126.905 kb on - strand at 1127.139 kb on + strand, within DDA3937_RS05040 at 1127.139 kb on + strand, within DDA3937_RS05040 at 1127.140 kb on - strand, within DDA3937_RS05040 at 1127.140 kb on - strand, within DDA3937_RS05040 at 1127.355 kb on + strand, within DDA3937_RS05040 at 1127.369 kb on + strand, within DDA3937_RS05040 at 1127.369 kb on + strand, within DDA3937_RS05040 at 1127.370 kb on - strand, within DDA3937_RS05040 at 1127.370 kb on - strand, within DDA3937_RS05040 at 1127.393 kb on + strand, within DDA3937_RS05040 at 1127.393 kb on + strand, within DDA3937_RS05040 at 1127.393 kb on + strand, within DDA3937_RS05040 at 1127.393 kb on + strand, within DDA3937_RS05040 at 1127.490 kb on + strand, within DDA3937_RS05040 at 1127.722 kb on + strand at 1127.723 kb on - strand, within DDA3937_RS05040 at 1127.723 kb on - strand, within DDA3937_RS05040
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,125,390 - -0.1 1,125,432 + -0.4 1,125,433 - +0.5 1,125,436 + +0.7 1,125,436 + -1.6 1,125,451 - +0.4 1,125,467 + -0.3 1,125,467 + -0.9 1,125,467 + -0.3 1,125,467 + +1.2 1,125,467 + -0.3 1,125,468 - +0.4 1,125,468 - +0.1 1,125,468 - -1.0 1,125,468 - +0.3 1,125,468 - -1.3 1,125,468 - +0.2 1,125,468 - -1.3 1,125,528 + -0.3 1,125,571 + +0.0 1,125,623 + -2.8 1,125,736 + DDA3937_RS05030 0.20 -0.0 1,125,772 + DDA3937_RS05030 0.25 +0.1 1,125,773 - DDA3937_RS05030 0.25 -3.2 1,125,901 + DDA3937_RS05030 0.40 -0.8 1,125,901 + DDA3937_RS05030 0.40 -0.2 1,125,902 - DDA3937_RS05030 0.40 +1.7 1,126,082 - DDA3937_RS05030 0.62 -0.6 1,126,082 - DDA3937_RS05030 0.62 +0.3 1,126,132 + DDA3937_RS05030 0.68 -1.1 1,126,132 + DDA3937_RS05030 0.68 +0.0 1,126,132 + DDA3937_RS05030 0.68 +1.3 1,126,142 - DDA3937_RS05030 0.69 -0.6 1,126,200 - DDA3937_RS05030 0.76 -0.8 1,126,200 - DDA3937_RS05030 0.76 -1.9 1,126,200 - DDA3937_RS05030 0.76 -1.7 1,126,235 - DDA3937_RS05030 0.80 -2.1 1,126,251 + DDA3937_RS05030 0.82 +0.4 1,126,258 + DDA3937_RS05030 0.83 +1.0 1,126,258 + DDA3937_RS05030 0.83 -0.9 1,126,258 + DDA3937_RS05030 0.83 -0.1 1,126,259 - DDA3937_RS05030 0.83 +1.7 1,126,259 - DDA3937_RS05030 0.83 -1.4 1,126,490 - DDA3937_RS05035 0.23 -0.5 1,126,537 + DDA3937_RS05035 0.34 -0.4 1,126,537 + +0.7 1,126,538 - DDA3937_RS05035 0.34 -0.8 1,126,719 + DDA3937_RS05035 0.75 -2.6 1,126,720 - DDA3937_RS05035 0.75 -1.5 1,126,847 - +0.7 1,126,904 + -0.0 1,126,904 + -0.1 1,126,904 + +2.5 1,126,905 - -0.7 1,126,905 - -1.9 1,127,139 + DDA3937_RS05040 0.25 -0.3 1,127,139 + DDA3937_RS05040 0.25 -1.0 1,127,140 - DDA3937_RS05040 0.25 -0.7 1,127,140 - DDA3937_RS05040 0.25 -1.1 1,127,355 + DDA3937_RS05040 0.43 -1.9 1,127,369 + DDA3937_RS05040 0.44 -0.9 1,127,369 + DDA3937_RS05040 0.44 -0.7 1,127,370 - DDA3937_RS05040 0.44 -0.5 1,127,370 - DDA3937_RS05040 0.44 -0.6 1,127,393 + DDA3937_RS05040 0.46 +0.4 1,127,393 + DDA3937_RS05040 0.46 -1.4 1,127,393 + DDA3937_RS05040 0.46 +0.0 1,127,393 + DDA3937_RS05040 0.46 -0.5 1,127,490 + DDA3937_RS05040 0.54 -0.3 1,127,722 + +0.7 1,127,723 - DDA3937_RS05040 0.74 -0.2 1,127,723 - DDA3937_RS05040 0.74 +0.0
Or see this region's nucleotide sequence