Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS05180 and DDA3937_RS05185 overlap by 4 nucleotides DDA3937_RS05185 and DDA3937_RS05190 are separated by 75 nucleotides DDA3937_RS05190 and DDA3937_RS05195 are separated by 12 nucleotides
DDA3937_RS05180: DDA3937_RS05180 - lipid-A-disaccharide synthase, at 1,155,414 to 1,156,562
_RS05180
DDA3937_RS05185: DDA3937_RS05185 - ribonuclease HII, at 1,156,559 to 1,157,155
_RS05185
DDA3937_RS05190: DDA3937_RS05190 - DNA polymerase III subunit alpha, at 1,157,231 to 1,160,713
_RS05190
DDA3937_RS05195: DDA3937_RS05195 - acetyl-CoA carboxylase carboxyl transferase subunit alpha, at 1,160,726 to 1,161,685
_RS05195
Position (kb)
1157
1158
1159
1160
1161 Strain fitness (log2 ratio)
-1
0
1
2 at 1156.594 kb on + strand at 1156.594 kb on + strand at 1156.632 kb on + strand, within DDA3937_RS05185 at 1156.733 kb on + strand, within DDA3937_RS05185 at 1156.733 kb on + strand, within DDA3937_RS05185 at 1156.804 kb on + strand, within DDA3937_RS05185 at 1156.861 kb on + strand, within DDA3937_RS05185 at 1156.861 kb on + strand, within DDA3937_RS05185 at 1156.862 kb on - strand, within DDA3937_RS05185 at 1157.060 kb on - strand, within DDA3937_RS05185 at 1157.066 kb on + strand, within DDA3937_RS05185 at 1157.175 kb on + strand at 1157.175 kb on + strand at 1157.175 kb on + strand at 1160.711 kb on + strand at 1161.677 kb on + strand at 1161.689 kb on - strand at 1161.697 kb on - strand at 1161.699 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,156,594 + +0.2 1,156,594 + +1.6 1,156,632 + DDA3937_RS05185 0.12 +0.1 1,156,733 + DDA3937_RS05185 0.29 +0.2 1,156,733 + DDA3937_RS05185 0.29 +0.0 1,156,804 + DDA3937_RS05185 0.41 +0.1 1,156,861 + DDA3937_RS05185 0.51 +0.4 1,156,861 + DDA3937_RS05185 0.51 -0.6 1,156,862 - DDA3937_RS05185 0.51 +0.0 1,157,060 - DDA3937_RS05185 0.84 +0.0 1,157,066 + DDA3937_RS05185 0.85 -1.0 1,157,175 + +1.0 1,157,175 + -0.3 1,157,175 + +2.0 1,160,711 + +0.6 1,161,677 + -1.6 1,161,689 - +0.6 1,161,697 - -1.0 1,161,699 - -0.5
Or see this region's nucleotide sequence