Experiment: 7.5mM_PTB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS11210 and DDA3937_RS11215 are separated by 3 nucleotides DDA3937_RS11215 and DDA3937_RS11220 are separated by 72 nucleotides DDA3937_RS11220 and DDA3937_RS11225 are separated by 198 nucleotides
DDA3937_RS11210: DDA3937_RS11210 - septum site-determining protein MinD, at 2,548,079 to 2,548,891
_RS11210
DDA3937_RS11215: DDA3937_RS11215 - cell division topological specificity factor MinE, at 2,548,895 to 2,549,176
_RS11215
DDA3937_RS11220: DDA3937_RS11220 - ribonuclease D, at 2,549,249 to 2,550,370
_RS11220
DDA3937_RS11225: DDA3937_RS11225 - long-chain-fatty-acid--CoA ligase FadD, at 2,550,569 to 2,552,238
_RS11225
Position (kb)
2549
2550
2551 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2548.250 kb on + strand, within DDA3937_RS11210 at 2548.250 kb on + strand, within DDA3937_RS11210 at 2548.250 kb on + strand at 2548.309 kb on + strand, within DDA3937_RS11210 at 2548.328 kb on + strand, within DDA3937_RS11210 at 2548.329 kb on - strand, within DDA3937_RS11210 at 2548.329 kb on - strand, within DDA3937_RS11210 at 2548.331 kb on - strand, within DDA3937_RS11210 at 2548.479 kb on - strand, within DDA3937_RS11210 at 2548.479 kb on - strand, within DDA3937_RS11210 at 2548.625 kb on + strand at 2548.794 kb on - strand, within DDA3937_RS11210 at 2549.174 kb on + strand at 2549.175 kb on - strand at 2549.175 kb on - strand at 2549.230 kb on + strand at 2549.248 kb on - strand at 2549.461 kb on + strand, within DDA3937_RS11220 at 2549.461 kb on + strand, within DDA3937_RS11220 at 2549.461 kb on + strand, within DDA3937_RS11220 at 2549.462 kb on - strand, within DDA3937_RS11220 at 2549.462 kb on - strand at 2549.462 kb on - strand, within DDA3937_RS11220 at 2549.462 kb on - strand at 2549.462 kb on - strand, within DDA3937_RS11220 at 2549.692 kb on + strand at 2549.693 kb on - strand, within DDA3937_RS11220 at 2549.898 kb on + strand, within DDA3937_RS11220 at 2549.898 kb on + strand, within DDA3937_RS11220 at 2549.920 kb on - strand, within DDA3937_RS11220 at 2550.024 kb on + strand, within DDA3937_RS11220 at 2550.024 kb on + strand, within DDA3937_RS11220 at 2550.024 kb on + strand, within DDA3937_RS11220 at 2550.232 kb on - strand, within DDA3937_RS11220 at 2550.232 kb on - strand, within DDA3937_RS11220 at 2550.261 kb on + strand at 2550.262 kb on - strand at 2550.262 kb on - strand at 2550.353 kb on - strand at 2550.364 kb on - strand at 2550.428 kb on + strand at 2550.428 kb on + strand at 2550.428 kb on + strand at 2550.429 kb on - strand at 2550.429 kb on - strand at 2550.429 kb on - strand at 2550.429 kb on - strand at 2550.432 kb on - strand at 2550.555 kb on - strand at 2550.589 kb on + strand at 2550.654 kb on - strand at 2550.668 kb on - strand at 2550.668 kb on - strand at 2550.683 kb on - strand at 2550.683 kb on - strand at 2550.956 kb on + strand, within DDA3937_RS11225 at 2550.956 kb on + strand, within DDA3937_RS11225 at 2550.957 kb on - strand, within DDA3937_RS11225 at 2550.957 kb on - strand at 2550.957 kb on - strand, within DDA3937_RS11225 at 2551.066 kb on + strand, within DDA3937_RS11225 at 2551.067 kb on - strand, within DDA3937_RS11225 at 2551.069 kb on + strand at 2551.069 kb on + strand at 2551.070 kb on - strand, within DDA3937_RS11225 at 2551.070 kb on - strand, within DDA3937_RS11225 at 2551.070 kb on - strand, within DDA3937_RS11225 at 2551.113 kb on + strand, within DDA3937_RS11225 at 2551.114 kb on - strand, within DDA3937_RS11225 at 2551.114 kb on - strand, within DDA3937_RS11225 at 2551.128 kb on + strand, within DDA3937_RS11225 at 2551.171 kb on - strand, within DDA3937_RS11225 at 2551.318 kb on + strand, within DDA3937_RS11225 at 2551.318 kb on + strand, within DDA3937_RS11225 at 2551.318 kb on + strand, within DDA3937_RS11225 at 2551.319 kb on - strand, within DDA3937_RS11225 at 2551.319 kb on - strand, within DDA3937_RS11225 at 2551.339 kb on + strand, within DDA3937_RS11225 at 2551.350 kb on + strand, within DDA3937_RS11225 at 2551.351 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7.5mM_PTB remove 2,548,250 + DDA3937_RS11210 0.21 -1.6 2,548,250 + DDA3937_RS11210 0.21 -1.2 2,548,250 + -2.8 2,548,309 + DDA3937_RS11210 0.28 -1.9 2,548,328 + DDA3937_RS11210 0.31 -1.5 2,548,329 - DDA3937_RS11210 0.31 -1.5 2,548,329 - DDA3937_RS11210 0.31 -1.8 2,548,331 - DDA3937_RS11210 0.31 -1.2 2,548,479 - DDA3937_RS11210 0.49 -0.2 2,548,479 - DDA3937_RS11210 0.49 -1.8 2,548,625 + -2.9 2,548,794 - DDA3937_RS11210 0.88 -1.8 2,549,174 + -1.5 2,549,175 - +1.1 2,549,175 - -2.1 2,549,230 + -2.6 2,549,248 - -1.1 2,549,461 + DDA3937_RS11220 0.19 +0.8 2,549,461 + DDA3937_RS11220 0.19 -2.4 2,549,461 + DDA3937_RS11220 0.19 -1.4 2,549,462 - DDA3937_RS11220 0.19 -1.1 2,549,462 - -0.5 2,549,462 - DDA3937_RS11220 0.19 -0.1 2,549,462 - -1.5 2,549,462 - DDA3937_RS11220 0.19 -1.3 2,549,692 + +0.5 2,549,693 - DDA3937_RS11220 0.40 -0.5 2,549,898 + DDA3937_RS11220 0.58 +0.2 2,549,898 + DDA3937_RS11220 0.58 +0.3 2,549,920 - DDA3937_RS11220 0.60 -2.5 2,550,024 + DDA3937_RS11220 0.69 -1.1 2,550,024 + DDA3937_RS11220 0.69 -1.5 2,550,024 + DDA3937_RS11220 0.69 +0.0 2,550,232 - DDA3937_RS11220 0.88 -2.3 2,550,232 - DDA3937_RS11220 0.88 -0.5 2,550,261 + -0.2 2,550,262 - -2.3 2,550,262 - +0.2 2,550,353 - -1.1 2,550,364 - -0.3 2,550,428 + +0.2 2,550,428 + -0.1 2,550,428 + -0.6 2,550,429 - +0.5 2,550,429 - +0.5 2,550,429 - +0.1 2,550,429 - -0.2 2,550,432 - -0.7 2,550,555 - +0.2 2,550,589 + +0.3 2,550,654 - -0.5 2,550,668 - +0.5 2,550,668 - +0.9 2,550,683 - -0.1 2,550,683 - +2.7 2,550,956 + DDA3937_RS11225 0.23 -0.3 2,550,956 + DDA3937_RS11225 0.23 -0.9 2,550,957 - DDA3937_RS11225 0.23 -1.1 2,550,957 - -0.1 2,550,957 - DDA3937_RS11225 0.23 -0.5 2,551,066 + DDA3937_RS11225 0.30 -0.2 2,551,067 - DDA3937_RS11225 0.30 -0.3 2,551,069 + +0.2 2,551,069 + -1.1 2,551,070 - DDA3937_RS11225 0.30 -0.2 2,551,070 - DDA3937_RS11225 0.30 +0.4 2,551,070 - DDA3937_RS11225 0.30 -0.9 2,551,113 + DDA3937_RS11225 0.33 -2.8 2,551,114 - DDA3937_RS11225 0.33 +0.0 2,551,114 - DDA3937_RS11225 0.33 +0.0 2,551,128 + DDA3937_RS11225 0.33 -1.3 2,551,171 - DDA3937_RS11225 0.36 +0.3 2,551,318 + DDA3937_RS11225 0.45 +0.0 2,551,318 + DDA3937_RS11225 0.45 +1.2 2,551,318 + DDA3937_RS11225 0.45 -0.7 2,551,319 - DDA3937_RS11225 0.45 +0.6 2,551,319 - DDA3937_RS11225 0.45 +1.5 2,551,339 + DDA3937_RS11225 0.46 -0.3 2,551,350 + DDA3937_RS11225 0.47 +0.7 2,551,351 - +1.0
Or see this region's nucleotide sequence