Experiment: 3.75mM_PTB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS04400 and DDA3937_RS21950 are separated by 121 nucleotides DDA3937_RS21950 and DDA3937_RS04405 are separated by 416 nucleotides DDA3937_RS04405 and DDA3937_RS04410 are separated by 35 nucleotides DDA3937_RS04410 and DDA3937_RS04415 are separated by 15 nucleotides
DDA3937_RS04400: DDA3937_RS04400 - glycosyltransferase, at 971,163 to 972,437
_RS04400
DDA3937_RS21950: DDA3937_RS21950 - helix-turn-helix domain-containing protein, at 972,559 to 972,846
_RS21950
DDA3937_RS04405: DDA3937_RS04405 - GNAT family N-acetyltransferase, at 973,263 to 973,676
_RS04405
DDA3937_RS04410: DDA3937_RS04410 - PAS and helix-turn-helix domain-containing protein, at 973,712 to 974,401
_RS04410
DDA3937_RS04415: DDA3937_RS04415 - cytosine permease, at 974,417 to 975,712
_RS04415
Position (kb) 973
974 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 972.351 kb on - strand at 972.455 kb on + strand at 972.455 kb on + strand at 972.455 kb on + strand at 972.456 kb on - strand at 972.459 kb on + strand at 972.459 kb on + strand at 972.460 kb on - strand at 972.460 kb on - strand at 972.495 kb on - strand at 972.529 kb on + strand at 972.529 kb on + strand at 972.579 kb on + strand at 972.588 kb on + strand, within DDA3937_RS21950 at 972.622 kb on - strand, within DDA3937_RS21950 at 972.642 kb on - strand, within DDA3937_RS21950 at 972.677 kb on - strand, within DDA3937_RS21950 at 972.677 kb on - strand, within DDA3937_RS21950 at 972.719 kb on + strand, within DDA3937_RS21950 at 972.719 kb on + strand, within DDA3937_RS21950 at 972.719 kb on + strand, within DDA3937_RS21950 at 972.720 kb on - strand at 972.720 kb on - strand, within DDA3937_RS21950 at 972.720 kb on - strand, within DDA3937_RS21950 at 972.720 kb on - strand, within DDA3937_RS21950 at 972.776 kb on - strand, within DDA3937_RS21950 at 972.784 kb on - strand, within DDA3937_RS21950 at 972.839 kb on - strand at 972.841 kb on - strand at 972.847 kb on + strand at 972.848 kb on - strand at 972.848 kb on - strand at 972.858 kb on + strand at 972.859 kb on - strand at 972.897 kb on + strand at 972.921 kb on + strand at 972.922 kb on - strand at 972.922 kb on - strand at 972.985 kb on + strand at 973.016 kb on + strand at 973.027 kb on + strand at 973.043 kb on - strand at 973.044 kb on + strand at 973.056 kb on + strand at 973.097 kb on + strand at 973.101 kb on - strand at 973.152 kb on - strand at 973.160 kb on - strand at 973.251 kb on - strand at 973.260 kb on - strand at 973.260 kb on - strand at 973.308 kb on + strand, within DDA3937_RS04405 at 973.308 kb on + strand, within DDA3937_RS04405 at 973.309 kb on - strand, within DDA3937_RS04405 at 973.334 kb on + strand, within DDA3937_RS04405 at 973.358 kb on + strand, within DDA3937_RS04405 at 973.453 kb on - strand, within DDA3937_RS04405 at 973.463 kb on + strand, within DDA3937_RS04405 at 973.464 kb on - strand, within DDA3937_RS04405 at 973.478 kb on + strand, within DDA3937_RS04405 at 973.479 kb on - strand, within DDA3937_RS04405 at 973.479 kb on - strand, within DDA3937_RS04405 at 973.544 kb on + strand, within DDA3937_RS04405 at 973.551 kb on + strand, within DDA3937_RS04405 at 973.551 kb on + strand, within DDA3937_RS04405 at 973.551 kb on + strand, within DDA3937_RS04405 at 973.552 kb on - strand, within DDA3937_RS04405 at 973.552 kb on - strand, within DDA3937_RS04405 at 973.552 kb on - strand, within DDA3937_RS04405 at 973.552 kb on - strand, within DDA3937_RS04405 at 973.552 kb on - strand, within DDA3937_RS04405 at 973.552 kb on - strand, within DDA3937_RS04405 at 973.566 kb on - strand, within DDA3937_RS04405 at 973.603 kb on - strand, within DDA3937_RS04405 at 973.612 kb on - strand, within DDA3937_RS04405 at 973.637 kb on + strand at 973.637 kb on + strand at 973.637 kb on + strand at 973.637 kb on + strand at 973.637 kb on + strand at 973.637 kb on + strand at 973.638 kb on - strand at 973.638 kb on - strand at 973.638 kb on - strand at 973.638 kb on - strand at 973.638 kb on - strand at 973.706 kb on - strand at 973.742 kb on + strand at 973.743 kb on - strand at 973.743 kb on - strand at 973.745 kb on - strand at 973.816 kb on + strand, within DDA3937_RS04410 at 973.820 kb on + strand, within DDA3937_RS04410 at 973.821 kb on - strand, within DDA3937_RS04410 at 973.844 kb on + strand, within DDA3937_RS04410 at 973.847 kb on - strand, within DDA3937_RS04410 at 973.997 kb on + strand, within DDA3937_RS04410 at 974.013 kb on + strand, within DDA3937_RS04410 at 974.112 kb on + strand, within DDA3937_RS04410 at 974.117 kb on + strand, within DDA3937_RS04410 at 974.158 kb on + strand, within DDA3937_RS04410 at 974.158 kb on + strand, within DDA3937_RS04410 at 974.159 kb on - strand, within DDA3937_RS04410 at 974.169 kb on + strand, within DDA3937_RS04410 at 974.289 kb on - strand, within DDA3937_RS04410 at 974.330 kb on + strand, within DDA3937_RS04410 at 974.360 kb on - strand at 974.391 kb on + strand at 974.395 kb on + strand at 974.395 kb on + strand at 974.395 kb on + strand at 974.396 kb on - strand at 974.396 kb on - strand at 974.400 kb on - strand at 974.527 kb on + strand at 974.527 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3.75mM_PTB remove 972,351 - -0.9 972,455 + +0.0 972,455 + -0.0 972,455 + -0.8 972,456 - +0.2 972,459 + -0.3 972,459 + -0.2 972,460 - -0.8 972,460 - -0.1 972,495 - +0.0 972,529 + +0.5 972,529 + +0.6 972,579 + -1.1 972,588 + DDA3937_RS21950 0.10 +0.7 972,622 - DDA3937_RS21950 0.22 -0.1 972,642 - DDA3937_RS21950 0.29 -0.4 972,677 - DDA3937_RS21950 0.41 -0.7 972,677 - DDA3937_RS21950 0.41 -1.5 972,719 + DDA3937_RS21950 0.56 -3.1 972,719 + DDA3937_RS21950 0.56 -0.4 972,719 + DDA3937_RS21950 0.56 -0.5 972,720 - +0.3 972,720 - DDA3937_RS21950 0.56 -0.0 972,720 - DDA3937_RS21950 0.56 -0.4 972,720 - DDA3937_RS21950 0.56 -0.3 972,776 - DDA3937_RS21950 0.75 +0.3 972,784 - DDA3937_RS21950 0.78 -0.7 972,839 - -0.9 972,841 - +0.6 972,847 + -2.1 972,848 - +0.2 972,848 - -0.3 972,858 + -1.0 972,859 - -0.9 972,897 + -1.9 972,921 + -1.7 972,922 - -0.3 972,922 - +0.3 972,985 + +0.9 973,016 + +0.9 973,027 + -1.0 973,043 - -0.2 973,044 + -0.7 973,056 + +0.9 973,097 + +0.2 973,101 - -0.1 973,152 - -0.4 973,160 - -2.3 973,251 - -0.6 973,260 - +0.3 973,260 - +1.6 973,308 + DDA3937_RS04405 0.11 -0.5 973,308 + DDA3937_RS04405 0.11 -1.7 973,309 - DDA3937_RS04405 0.11 +1.0 973,334 + DDA3937_RS04405 0.17 +0.1 973,358 + DDA3937_RS04405 0.23 -0.7 973,453 - DDA3937_RS04405 0.46 -0.5 973,463 + DDA3937_RS04405 0.48 -0.8 973,464 - DDA3937_RS04405 0.49 -1.1 973,478 + DDA3937_RS04405 0.52 +0.9 973,479 - DDA3937_RS04405 0.52 -0.6 973,479 - DDA3937_RS04405 0.52 +0.1 973,544 + DDA3937_RS04405 0.68 -0.0 973,551 + DDA3937_RS04405 0.70 -0.2 973,551 + DDA3937_RS04405 0.70 -0.1 973,551 + DDA3937_RS04405 0.70 -0.9 973,552 - DDA3937_RS04405 0.70 +0.6 973,552 - DDA3937_RS04405 0.70 -0.1 973,552 - DDA3937_RS04405 0.70 +0.2 973,552 - DDA3937_RS04405 0.70 -0.5 973,552 - DDA3937_RS04405 0.70 +0.9 973,552 - DDA3937_RS04405 0.70 -0.7 973,566 - DDA3937_RS04405 0.73 -0.7 973,603 - DDA3937_RS04405 0.82 +0.3 973,612 - DDA3937_RS04405 0.84 -0.3 973,637 + +0.3 973,637 + -0.0 973,637 + -0.5 973,637 + +0.1 973,637 + -0.5 973,637 + +0.5 973,638 - -0.3 973,638 - +0.5 973,638 - -0.1 973,638 - +0.6 973,638 - +0.0 973,706 - -1.0 973,742 + +1.3 973,743 - -0.2 973,743 - -1.4 973,745 - +0.2 973,816 + DDA3937_RS04410 0.15 +0.6 973,820 + DDA3937_RS04410 0.16 -0.7 973,821 - DDA3937_RS04410 0.16 -0.3 973,844 + DDA3937_RS04410 0.19 -1.7 973,847 - DDA3937_RS04410 0.20 -0.6 973,997 + DDA3937_RS04410 0.41 +0.1 974,013 + DDA3937_RS04410 0.44 -1.2 974,112 + DDA3937_RS04410 0.58 +2.9 974,117 + DDA3937_RS04410 0.59 +0.1 974,158 + DDA3937_RS04410 0.65 -0.7 974,158 + DDA3937_RS04410 0.65 -1.7 974,159 - DDA3937_RS04410 0.65 -0.3 974,169 + DDA3937_RS04410 0.66 -0.1 974,289 - DDA3937_RS04410 0.84 -0.8 974,330 + DDA3937_RS04410 0.90 -1.8 974,360 - -0.9 974,391 + +0.1 974,395 + -0.8 974,395 + -0.3 974,395 + +0.1 974,396 - +0.1 974,396 - +0.1 974,400 - -0.7 974,527 + -0.6 974,527 + -0.5
Or see this region's nucleotide sequence