Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS03300 and DDA3937_RS03305 are separated by 88 nucleotides DDA3937_RS03305 and DDA3937_RS03310 are separated by 40 nucleotides DDA3937_RS03310 and DDA3937_RS03315 are separated by 168 nucleotides
DDA3937_RS03300: DDA3937_RS03300 - site-specific tyrosine recombinase XerD, at 720,584 to 721,483
_RS03300
DDA3937_RS03305: DDA3937_RS03305 - bifunctional protein-disulfide isomerase/oxidoreductase DsbC, at 721,572 to 722,288
_RS03305
DDA3937_RS03310: DDA3937_RS03310 - single-stranded-DNA-specific exonuclease RecJ, at 722,329 to 724,059
_RS03310
DDA3937_RS03315: DDA3937_RS03315 - peptide chain release factor 2, at 724,228 to 725,326
_RS03315
Position (kb)
722
723
724
725 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 721.534 kb on + strand at 721.558 kb on - strand at 721.565 kb on + strand at 721.566 kb on - strand at 721.566 kb on - strand at 721.566 kb on - strand at 721.585 kb on + strand at 721.585 kb on + strand at 721.585 kb on + strand at 721.585 kb on + strand at 721.585 kb on + strand at 721.588 kb on + strand at 721.588 kb on + strand at 721.588 kb on + strand at 721.588 kb on + strand at 721.589 kb on - strand at 721.589 kb on - strand at 721.589 kb on - strand at 721.589 kb on - strand at 721.589 kb on - strand at 721.589 kb on - strand at 721.589 kb on - strand at 721.661 kb on - strand, within DDA3937_RS03305 at 721.661 kb on - strand, within DDA3937_RS03305 at 721.661 kb on - strand, within DDA3937_RS03305 at 721.661 kb on - strand, within DDA3937_RS03305 at 721.746 kb on + strand, within DDA3937_RS03305 at 721.746 kb on + strand, within DDA3937_RS03305 at 721.746 kb on + strand, within DDA3937_RS03305 at 721.746 kb on + strand, within DDA3937_RS03305 at 721.746 kb on + strand, within DDA3937_RS03305 at 721.746 kb on + strand, within DDA3937_RS03305 at 721.746 kb on + strand, within DDA3937_RS03305 at 721.746 kb on + strand, within DDA3937_RS03305 at 721.746 kb on + strand, within DDA3937_RS03305 at 721.746 kb on + strand, within DDA3937_RS03305 at 721.746 kb on + strand, within DDA3937_RS03305 at 721.746 kb on + strand, within DDA3937_RS03305 at 721.746 kb on + strand, within DDA3937_RS03305 at 721.746 kb on + strand at 721.747 kb on - strand, within DDA3937_RS03305 at 721.747 kb on - strand, within DDA3937_RS03305 at 721.747 kb on - strand, within DDA3937_RS03305 at 721.747 kb on - strand, within DDA3937_RS03305 at 721.747 kb on - strand, within DDA3937_RS03305 at 721.747 kb on - strand, within DDA3937_RS03305 at 721.747 kb on - strand, within DDA3937_RS03305 at 721.748 kb on + strand, within DDA3937_RS03305 at 721.748 kb on + strand, within DDA3937_RS03305 at 721.749 kb on - strand at 721.788 kb on + strand, within DDA3937_RS03305 at 721.788 kb on + strand, within DDA3937_RS03305 at 721.788 kb on + strand, within DDA3937_RS03305 at 721.788 kb on + strand, within DDA3937_RS03305 at 721.788 kb on + strand, within DDA3937_RS03305 at 721.789 kb on - strand, within DDA3937_RS03305 at 721.789 kb on - strand, within DDA3937_RS03305 at 721.789 kb on - strand at 721.789 kb on - strand, within DDA3937_RS03305 at 721.789 kb on - strand, within DDA3937_RS03305 at 721.875 kb on + strand, within DDA3937_RS03305 at 721.875 kb on + strand, within DDA3937_RS03305 at 721.876 kb on - strand, within DDA3937_RS03305 at 721.877 kb on + strand, within DDA3937_RS03305 at 721.877 kb on + strand, within DDA3937_RS03305 at 721.922 kb on + strand at 721.922 kb on + strand, within DDA3937_RS03305 at 721.922 kb on + strand, within DDA3937_RS03305 at 721.922 kb on + strand, within DDA3937_RS03305 at 721.922 kb on + strand at 721.923 kb on - strand, within DDA3937_RS03305 at 721.923 kb on - strand, within DDA3937_RS03305 at 721.923 kb on - strand, within DDA3937_RS03305 at 721.965 kb on + strand, within DDA3937_RS03305 at 721.966 kb on - strand, within DDA3937_RS03305 at 721.966 kb on - strand, within DDA3937_RS03305 at 721.966 kb on - strand, within DDA3937_RS03305 at 721.992 kb on + strand, within DDA3937_RS03305 at 722.091 kb on - strand, within DDA3937_RS03305 at 722.123 kb on + strand, within DDA3937_RS03305 at 722.123 kb on + strand, within DDA3937_RS03305 at 722.124 kb on - strand, within DDA3937_RS03305 at 722.124 kb on - strand, within DDA3937_RS03305 at 722.124 kb on - strand, within DDA3937_RS03305 at 722.303 kb on + strand at 722.412 kb on - strand at 722.412 kb on - strand at 722.413 kb on + strand at 722.414 kb on - strand at 722.613 kb on + strand, within DDA3937_RS03310 at 722.614 kb on - strand, within DDA3937_RS03310 at 722.614 kb on - strand, within DDA3937_RS03310 at 722.638 kb on + strand, within DDA3937_RS03310 at 722.743 kb on - strand, within DDA3937_RS03310 at 723.060 kb on + strand, within DDA3937_RS03310 at 723.080 kb on + strand, within DDA3937_RS03310 at 723.081 kb on - strand, within DDA3937_RS03310 at 723.222 kb on + strand, within DDA3937_RS03310 at 723.222 kb on + strand, within DDA3937_RS03310 at 723.223 kb on - strand, within DDA3937_RS03310 at 723.223 kb on - strand, within DDA3937_RS03310 at 723.223 kb on - strand, within DDA3937_RS03310 at 723.223 kb on - strand, within DDA3937_RS03310 at 723.223 kb on - strand, within DDA3937_RS03310 at 723.223 kb on - strand, within DDA3937_RS03310 at 723.252 kb on + strand, within DDA3937_RS03310 at 723.338 kb on + strand, within DDA3937_RS03310 at 723.338 kb on + strand, within DDA3937_RS03310 at 723.338 kb on + strand, within DDA3937_RS03310 at 723.406 kb on + strand, within DDA3937_RS03310 at 723.406 kb on + strand, within DDA3937_RS03310 at 723.406 kb on + strand, within DDA3937_RS03310 at 723.406 kb on + strand, within DDA3937_RS03310 at 723.406 kb on + strand, within DDA3937_RS03310 at 723.407 kb on - strand, within DDA3937_RS03310 at 723.407 kb on - strand, within DDA3937_RS03310 at 723.985 kb on + strand at 723.986 kb on - strand at 724.025 kb on + strand at 724.026 kb on - strand at 724.079 kb on - strand at 724.122 kb on - strand at 724.130 kb on - strand at 724.138 kb on + strand at 724.138 kb on + strand at 724.138 kb on + strand at 724.148 kb on + strand at 724.148 kb on + strand at 724.183 kb on + strand at 724.183 kb on + strand at 724.183 kb on + strand at 724.183 kb on + strand at 724.192 kb on + strand at 724.192 kb on + strand at 724.213 kb on + strand at 724.213 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 721,534 + +0.3 721,558 - +1.0 721,565 + +1.3 721,566 - +1.5 721,566 - -0.3 721,566 - -0.2 721,585 + +1.3 721,585 + +0.7 721,585 + +0.2 721,585 + +0.7 721,585 + +0.7 721,588 + -0.8 721,588 + -0.7 721,588 + +1.7 721,588 + -0.3 721,589 - +0.8 721,589 - +0.5 721,589 - -0.3 721,589 - -1.4 721,589 - +0.2 721,589 - -0.7 721,589 - +1.7 721,661 - DDA3937_RS03305 0.12 -0.1 721,661 - DDA3937_RS03305 0.12 +0.8 721,661 - DDA3937_RS03305 0.12 +0.5 721,661 - DDA3937_RS03305 0.12 +0.4 721,746 + DDA3937_RS03305 0.24 -0.1 721,746 + DDA3937_RS03305 0.24 +1.7 721,746 + DDA3937_RS03305 0.24 +0.9 721,746 + DDA3937_RS03305 0.24 -0.8 721,746 + DDA3937_RS03305 0.24 -0.4 721,746 + DDA3937_RS03305 0.24 -0.4 721,746 + DDA3937_RS03305 0.24 +0.5 721,746 + DDA3937_RS03305 0.24 -0.6 721,746 + DDA3937_RS03305 0.24 +0.3 721,746 + DDA3937_RS03305 0.24 -0.8 721,746 + DDA3937_RS03305 0.24 +0.5 721,746 + DDA3937_RS03305 0.24 +0.4 721,746 + DDA3937_RS03305 0.24 +1.5 721,746 + +0.4 721,747 - DDA3937_RS03305 0.24 +1.7 721,747 - DDA3937_RS03305 0.24 -0.0 721,747 - DDA3937_RS03305 0.24 +0.2 721,747 - DDA3937_RS03305 0.24 +0.7 721,747 - DDA3937_RS03305 0.24 +0.9 721,747 - DDA3937_RS03305 0.24 -0.3 721,747 - DDA3937_RS03305 0.24 -2.0 721,748 + DDA3937_RS03305 0.25 +0.2 721,748 + DDA3937_RS03305 0.25 -0.2 721,749 - -0.2 721,788 + DDA3937_RS03305 0.30 -0.2 721,788 + DDA3937_RS03305 0.30 -0.2 721,788 + DDA3937_RS03305 0.30 +0.7 721,788 + DDA3937_RS03305 0.30 +1.7 721,788 + DDA3937_RS03305 0.30 +0.5 721,789 - DDA3937_RS03305 0.30 +0.7 721,789 - DDA3937_RS03305 0.30 +0.7 721,789 - +0.2 721,789 - DDA3937_RS03305 0.30 +0.3 721,789 - DDA3937_RS03305 0.30 -0.3 721,875 + DDA3937_RS03305 0.42 +1.1 721,875 + DDA3937_RS03305 0.42 +0.5 721,876 - DDA3937_RS03305 0.42 -0.3 721,877 + DDA3937_RS03305 0.43 +2.0 721,877 + DDA3937_RS03305 0.43 +0.2 721,922 + +0.2 721,922 + DDA3937_RS03305 0.49 +0.2 721,922 + DDA3937_RS03305 0.49 +0.2 721,922 + DDA3937_RS03305 0.49 -0.3 721,922 + +0.5 721,923 - DDA3937_RS03305 0.49 +1.2 721,923 - DDA3937_RS03305 0.49 +0.2 721,923 - DDA3937_RS03305 0.49 -0.0 721,965 + DDA3937_RS03305 0.55 +0.6 721,966 - DDA3937_RS03305 0.55 +1.4 721,966 - DDA3937_RS03305 0.55 +1.0 721,966 - DDA3937_RS03305 0.55 +0.4 721,992 + DDA3937_RS03305 0.59 +1.7 722,091 - DDA3937_RS03305 0.72 -0.5 722,123 + DDA3937_RS03305 0.77 -0.2 722,123 + DDA3937_RS03305 0.77 -0.8 722,124 - DDA3937_RS03305 0.77 +0.0 722,124 - DDA3937_RS03305 0.77 +0.7 722,124 - DDA3937_RS03305 0.77 +0.6 722,303 + +0.7 722,412 - -0.9 722,412 - -2.0 722,413 + -1.1 722,414 - -1.1 722,613 + DDA3937_RS03310 0.16 -0.2 722,614 - DDA3937_RS03310 0.16 -0.4 722,614 - DDA3937_RS03310 0.16 -1.7 722,638 + DDA3937_RS03310 0.18 -0.8 722,743 - DDA3937_RS03310 0.24 +0.6 723,060 + DDA3937_RS03310 0.42 +0.6 723,080 + DDA3937_RS03310 0.43 -0.8 723,081 - DDA3937_RS03310 0.43 +0.2 723,222 + DDA3937_RS03310 0.52 +0.7 723,222 + DDA3937_RS03310 0.52 -0.4 723,223 - DDA3937_RS03310 0.52 -0.1 723,223 - DDA3937_RS03310 0.52 -0.2 723,223 - DDA3937_RS03310 0.52 +0.2 723,223 - DDA3937_RS03310 0.52 +0.9 723,223 - DDA3937_RS03310 0.52 -0.8 723,223 - DDA3937_RS03310 0.52 -0.4 723,252 + DDA3937_RS03310 0.53 -1.0 723,338 + DDA3937_RS03310 0.58 +0.2 723,338 + DDA3937_RS03310 0.58 -0.1 723,338 + DDA3937_RS03310 0.58 -0.3 723,406 + DDA3937_RS03310 0.62 -1.8 723,406 + DDA3937_RS03310 0.62 -1.8 723,406 + DDA3937_RS03310 0.62 -0.8 723,406 + DDA3937_RS03310 0.62 -1.5 723,406 + DDA3937_RS03310 0.62 -1.2 723,407 - DDA3937_RS03310 0.62 -1.3 723,407 - DDA3937_RS03310 0.62 -0.1 723,985 + -0.7 723,986 - -1.4 724,025 + -0.3 724,026 - +0.5 724,079 - -0.3 724,122 - -1.0 724,130 - -0.4 724,138 + +0.7 724,138 + +0.3 724,138 + +0.0 724,148 + +0.3 724,148 + -1.5 724,183 + -0.0 724,183 + +0.8 724,183 + -0.1 724,183 + +0.3 724,192 + +1.0 724,192 + -0.1 724,213 + -0.7 724,213 + -1.8
Or see this region's nucleotide sequence