Strain Fitness in Escherichia coli BL21 around ECD_10005

Experiment: Control_TnN

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_10004 and ECD_10005 are separated by 908 nucleotidesECD_10005 and ECD_10006 are separated by 39 nucleotidesECD_10006 and ECD_10007 are separated by 88 nucleotidesECD_10007 and ECD_10008 are separated by 191 nucleotides ECD_10004: ECD_10004 - T7 DNA-directed RNA polymerase, at 750,421 to 753,072 _10004 ECD_10005: ECD_10005 - Excisionase, at 753,981 to 754,199 _10005 ECD_10006: ECD_10006 - hypothetical protein, at 754,239 to 754,406 _10006 ECD_10007: ECD_10007 - ea8.5, at 754,495 to 754,776 _10007 ECD_10008: ECD_10008 - ea22, at 754,968 to 755,516 _10008 Position (kb) 753 754 755Strain fitness (log2 ratio) -2 -1 0 1 2at 753.142 kb on - strandat 753.150 kb on - strandat 753.182 kb on - strandat 753.192 kb on - strandat 753.316 kb on - strandat 753.330 kb on - strandat 753.524 kb on + strandat 753.525 kb on - strandat 753.525 kb on - strandat 753.526 kb on + strandat 753.526 kb on + strandat 753.526 kb on + strandat 753.526 kb on + strandat 753.526 kb on + strandat 753.526 kb on + strandat 753.527 kb on - strandat 753.527 kb on - strandat 753.609 kb on - strandat 753.609 kb on - strandat 753.706 kb on - strandat 753.710 kb on + strandat 753.777 kb on - strandat 753.791 kb on - strandat 753.791 kb on - strandat 753.857 kb on + strandat 753.951 kb on - strandat 753.951 kb on - strandat 753.951 kb on - strandat 753.955 kb on - strandat 754.111 kb on - strand, within ECD_10005at 754.196 kb on - strandat 754.196 kb on - strandat 754.196 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_TnN
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753,142 - +0.8
753,150 - +0.3
753,182 - -0.3
753,192 - -0.4
753,316 - -0.5
753,330 - -0.6
753,524 + +0.2
753,525 - +0.0
753,525 - +0.9
753,526 + +2.0
753,526 + -0.2
753,526 + +0.9
753,526 + -0.5
753,526 + +1.3
753,526 + -0.1
753,527 - +0.0
753,527 - +0.3
753,609 - -0.3
753,609 - +0.0
753,706 - +0.5
753,710 + +0.7
753,777 - -0.9
753,791 - +0.1
753,791 - -0.3
753,857 + +0.2
753,951 - -0.0
753,951 - +0.5
753,951 - +0.0
753,955 - -0.2
754,111 - ECD_10005 0.59 -1.4
754,196 - -1.0
754,196 - +0.3
754,196 - -1.9

Or see this region's nucleotide sequence