Strain Fitness in Escherichia coli BL21 around ECD_00459

Experiment: Control_TnN

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00457 and ECD_00458 are separated by 12 nucleotidesECD_00458 and ECD_00459 are separated by 99 nucleotidesECD_00459 and ECD_00460 are separated by 31 nucleotidesECD_00460 and ECD_00461 overlap by 142 nucleotides ECD_00457: ECD_00457 - glyoxylate carboligase, at 503,061 to 504,842 _00457 ECD_00458: ECD_00458 - hydroxypyruvate isomerase, at 504,855 to 505,631 _00458 ECD_00459: ECD_00459 - tartronate semialdehyde reductase, NADH-dependent, at 505,731 to 506,609 _00459 ECD_00460: ECD_00460 - hypothetical protein, at 506,641 to 506,919 _00460 ECD_00461: ECD_00461 - putative allantoin transporter, at 506,778 to 508,232 _00461 Position (kb) 505 506 507Strain fitness (log2 ratio) -1 0 1at 505.807 kb on + strandat 505.807 kb on + strandat 506.004 kb on - strand, within ECD_00459at 506.129 kb on + strand, within ECD_00459at 506.165 kb on + strand, within ECD_00459at 506.166 kb on - strand, within ECD_00459at 506.515 kb on + strand, within ECD_00459at 507.079 kb on + strand, within ECD_00461at 507.265 kb on + strand, within ECD_00461at 507.351 kb on + strand, within ECD_00461at 507.484 kb on + strand, within ECD_00461

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_TnN
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505,807 + +0.5
505,807 + -1.3
506,004 - ECD_00459 0.31 +0.2
506,129 + ECD_00459 0.45 -1.0
506,165 + ECD_00459 0.49 -0.7
506,166 - ECD_00459 0.49 +0.8
506,515 + ECD_00459 0.89 +1.2
507,079 + ECD_00461 0.21 -0.4
507,265 + ECD_00461 0.33 -0.1
507,351 + ECD_00461 0.39 -0.2
507,484 + ECD_00461 0.49 -0.3

Or see this region's nucleotide sequence