Strain Fitness in Escherichia coli BL21 around ECD_00188

Experiment: HSDP3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00186 and ECD_00187 are separated by 48 nucleotidesECD_00187 and ECD_00188 overlap by 8 nucleotidesECD_00188 and ECD_00189 are separated by 165 nucleotidesECD_00189 and ECD_00190 overlap by 4 nucleotides ECD_00186: ECD_00186 - tRNA(Ile)-lysidine synthetase, at 215,174 to 216,472 _00186 ECD_00187: ECD_00187 - modulator of Rho-dependent transcription termination, at 216,521 to 216,775 _00187 ECD_00188: ECD_00188 - UPF0253 family protein, at 216,768 to 216,968 _00188 ECD_00189: ECD_00189 - PDDEXK superfamily protein, at 217,134 to 217,679 _00189 ECD_00190: ECD_00190 - alternative stalled-ribosome rescue factor B; peptidyl-tRNA hydrolase, ribosome-attached, at 217,676 to 218,098 _00190 Position (kb) 216 217Strain fitness (log2 ratio) -2 -1 0 1at 216.466 kb on + strandat 216.467 kb on - strandat 216.471 kb on - strandat 216.562 kb on + strand, within ECD_00187at 216.587 kb on + strand, within ECD_00187at 216.624 kb on + strand, within ECD_00187at 216.625 kb on - strand, within ECD_00187at 216.625 kb on - strand, within ECD_00187at 216.704 kb on + strand, within ECD_00187at 216.705 kb on - strand, within ECD_00187at 216.739 kb on - strand, within ECD_00187at 216.739 kb on - strand, within ECD_00187at 216.763 kb on - strandat 216.767 kb on - strandat 216.767 kb on - strandat 216.811 kb on + strand, within ECD_00188at 216.812 kb on - strand, within ECD_00188at 216.910 kb on - strand, within ECD_00188at 216.910 kb on - strand, within ECD_00188at 216.959 kb on - strandat 217.012 kb on + strandat 217.049 kb on + strandat 217.050 kb on - strandat 217.050 kb on - strandat 217.051 kb on + strandat 217.051 kb on + strandat 217.051 kb on + strandat 217.051 kb on + strandat 217.052 kb on - strandat 217.052 kb on - strandat 217.052 kb on - strandat 217.052 kb on - strandat 217.093 kb on - strandat 217.250 kb on + strand, within ECD_00189at 217.251 kb on - strand, within ECD_00189at 217.281 kb on + strand, within ECD_00189at 217.281 kb on + strand, within ECD_00189at 217.452 kb on + strand, within ECD_00189at 217.452 kb on + strand, within ECD_00189at 217.452 kb on + strand, within ECD_00189at 217.452 kb on + strand, within ECD_00189at 217.453 kb on - strand, within ECD_00189at 217.453 kb on - strand, within ECD_00189at 217.454 kb on + strand, within ECD_00189at 217.454 kb on + strand, within ECD_00189at 217.638 kb on - strandat 217.769 kb on - strand, within ECD_00190at 217.826 kb on + strand, within ECD_00190at 217.827 kb on - strand, within ECD_00190at 217.829 kb on + strand, within ECD_00190at 217.829 kb on + strand, within ECD_00190at 217.968 kb on + strand, within ECD_00190at 217.968 kb on + strand, within ECD_00190

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Per-strain Table

Position Strand Gene LocusTag Fraction HSDP3
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216,466 + -1.7
216,467 - +1.5
216,471 - -0.1
216,562 + ECD_00187 0.16 +1.3
216,587 + ECD_00187 0.26 +0.1
216,624 + ECD_00187 0.40 -0.7
216,625 - ECD_00187 0.41 +0.0
216,625 - ECD_00187 0.41 -1.6
216,704 + ECD_00187 0.72 +1.0
216,705 - ECD_00187 0.72 +0.2
216,739 - ECD_00187 0.85 -0.1
216,739 - ECD_00187 0.85 -0.2
216,763 - -1.1
216,767 - +0.4
216,767 - +1.4
216,811 + ECD_00188 0.21 -0.3
216,812 - ECD_00188 0.22 -0.4
216,910 - ECD_00188 0.71 -0.2
216,910 - ECD_00188 0.71 -0.6
216,959 - +0.4
217,012 + -0.4
217,049 + -0.4
217,050 - +0.5
217,050 - -1.8
217,051 + +0.7
217,051 + -0.6
217,051 + -0.1
217,051 + -0.3
217,052 - +0.7
217,052 - +0.0
217,052 - +0.3
217,052 - +0.6
217,093 - +0.5
217,250 + ECD_00189 0.21 +1.0
217,251 - ECD_00189 0.21 -0.6
217,281 + ECD_00189 0.27 +1.1
217,281 + ECD_00189 0.27 +1.6
217,452 + ECD_00189 0.58 -0.2
217,452 + ECD_00189 0.58 +1.1
217,452 + ECD_00189 0.58 +0.2
217,452 + ECD_00189 0.58 -0.2
217,453 - ECD_00189 0.58 -0.7
217,453 - ECD_00189 0.58 +0.6
217,454 + ECD_00189 0.59 +0.2
217,454 + ECD_00189 0.59 +0.1
217,638 - +0.4
217,769 - ECD_00190 0.22 -0.0
217,826 + ECD_00190 0.35 +0.0
217,827 - ECD_00190 0.36 -0.4
217,829 + ECD_00190 0.36 +0.1
217,829 + ECD_00190 0.36 +0.2
217,968 + ECD_00190 0.69 +0.4
217,968 + ECD_00190 0.69 +0.4

Or see this region's nucleotide sequence