Strain Fitness in Escherichia coli BL21 around ECD_01248

Experiment: Bas33

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01246 and ECD_01247 overlap by 4 nucleotidesECD_01247 and ECD_01248 are separated by 219 nucleotidesECD_01248 and ECD_01249 are separated by 35 nucleotidesECD_01249 and ECD_01250 are separated by 379 nucleotides ECD_01246: ECD_01246 - cob(I)yrinic acid a,c-diamide adenosyltransferase, at 1,314,137 to 1,314,727 _01246 ECD_01247: ECD_01247 - putative EmrKY-TolC system oxoacyl-(acyl carrier protein) reductase, at 1,314,724 to 1,315,482 _01247 ECD_01248: ECD_01248 - inner membrane protein, S49 peptidase family protein, at 1,315,702 to 1,316,751 _01248 ECD_01249: ECD_01249 - DUF2498 protein YciN, at 1,316,787 to 1,317,038 _01249 ECD_01250: ECD_01250 - DNA topoisomerase I, omega subunit, at 1,317,418 to 1,320,015 _01250 Position (kb) 1315 1316 1317Strain fitness (log2 ratio) -2 -1 0 1at 1314.990 kb on - strand, within ECD_01247at 1314.990 kb on - strand, within ECD_01247at 1315.036 kb on - strand, within ECD_01247at 1315.070 kb on - strand, within ECD_01247at 1315.316 kb on - strand, within ECD_01247at 1315.523 kb on - strandat 1315.528 kb on - strandat 1315.647 kb on - strandat 1315.803 kb on + strandat 1315.852 kb on + strand, within ECD_01248at 1315.854 kb on + strand, within ECD_01248at 1315.938 kb on + strand, within ECD_01248at 1316.639 kb on + strand, within ECD_01248at 1316.644 kb on + strand, within ECD_01248at 1317.040 kb on - strandat 1317.043 kb on + strandat 1317.177 kb on + strandat 1317.178 kb on - strandat 1317.404 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas33
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1,314,990 - ECD_01247 0.35 -0.1
1,314,990 - ECD_01247 0.35 -0.0
1,315,036 - ECD_01247 0.41 -0.3
1,315,070 - ECD_01247 0.46 -1.9
1,315,316 - ECD_01247 0.78 +0.3
1,315,523 - +0.0
1,315,528 - -0.6
1,315,647 - +1.1
1,315,803 + +0.1
1,315,852 + ECD_01248 0.14 -0.5
1,315,854 + ECD_01248 0.14 +0.2
1,315,938 + ECD_01248 0.22 -1.8
1,316,639 + ECD_01248 0.89 -0.7
1,316,644 + ECD_01248 0.90 -0.8
1,317,040 - -0.5
1,317,043 + +1.4
1,317,177 + +0.6
1,317,178 - -1.1
1,317,404 + +0.9

Or see this region's nucleotide sequence