Strain Fitness in Escherichia coli BL21 around ECD_00933

Experiment: Bas33

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00932 and ECD_00933 are separated by 184 nucleotidesECD_00933 and ECD_00934 are separated by 601 nucleotides ECD_00932: ECD_00932 - aspartate aminotransferase, PLP-dependent, at 989,609 to 990,799 _00932 ECD_00933: ECD_00933 - outer membrane porin 1a (Ia, at 990,984 to 992,072 _00933 ECD_00934: ECD_00934 - asparaginyl tRNA synthetase, at 992,674 to 994,074 _00934 Position (kb) 990 991 992 993Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 991.070 kb on - strandat 991.079 kb on - strandat 991.079 kb on - strandat 991.184 kb on - strand, within ECD_00933at 991.331 kb on - strand, within ECD_00933at 991.436 kb on - strand, within ECD_00933at 991.476 kb on - strand, within ECD_00933at 991.587 kb on - strand, within ECD_00933at 991.592 kb on - strand, within ECD_00933at 991.592 kb on - strand, within ECD_00933at 991.596 kb on - strand, within ECD_00933at 991.596 kb on - strand, within ECD_00933at 991.637 kb on - strand, within ECD_00933at 991.691 kb on - strand, within ECD_00933at 991.703 kb on - strand, within ECD_00933at 991.703 kb on - strand, within ECD_00933at 991.739 kb on - strand, within ECD_00933at 991.743 kb on - strand, within ECD_00933at 991.743 kb on - strand, within ECD_00933at 992.078 kb on - strandat 992.078 kb on - strandat 992.481 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas33
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991,070 - +2.9
991,079 - -1.9
991,079 - -1.2
991,184 - ECD_00933 0.18 -1.1
991,331 - ECD_00933 0.32 -0.6
991,436 - ECD_00933 0.42 -0.6
991,476 - ECD_00933 0.45 +0.1
991,587 - ECD_00933 0.55 -1.8
991,592 - ECD_00933 0.56 -0.4
991,592 - ECD_00933 0.56 -0.6
991,596 - ECD_00933 0.56 -0.2
991,596 - ECD_00933 0.56 +0.9
991,637 - ECD_00933 0.60 -1.4
991,691 - ECD_00933 0.65 +1.4
991,703 - ECD_00933 0.66 -0.6
991,703 - ECD_00933 0.66 -1.1
991,739 - ECD_00933 0.69 -0.6
991,743 - ECD_00933 0.70 -3.1
991,743 - ECD_00933 0.70 -0.1
992,078 - -1.6
992,078 - -1.2
992,481 - -0.7

Or see this region's nucleotide sequence