Strain Fitness in Escherichia coli BL21 around ECD_00925

Experiment: Bas33

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00923 and ECD_00924 overlap by 24 nucleotidesECD_00924 and ECD_00925 are separated by 135 nucleotidesECD_00925 and ECD_00926 overlap by 4 nucleotides ECD_00923: ECD_00923 - protein kinase-like domain protein, at 976,842 to 977,735 _00923 ECD_00924: ECD_00924 - envelope biogenesis factor; DUF218 superfamily protein, at 977,712 to 978,491 _00924 ECD_00925: ECD_00925 - putative S-adenosyl-L-methionine-dependent methyltransferase, at 978,627 to 979,412 _00925 ECD_00926: ECD_00926 - chromosome condensin MukBEF, kleisin-like subunit, binds calcium, at 979,409 to 980,731 _00926 Position (kb) 978 979 980Strain fitness (log2 ratio) -2 -1 0 1at 977.634 kb on + strand, within ECD_00923at 978.183 kb on - strand, within ECD_00924at 978.516 kb on + strandat 978.516 kb on + strandat 978.518 kb on + strandat 978.518 kb on + strandat 978.518 kb on + strandat 978.519 kb on - strandat 978.538 kb on + strandat 978.538 kb on + strandat 978.541 kb on + strandat 978.541 kb on + strandat 978.541 kb on + strandat 978.919 kb on + strand, within ECD_00925at 979.018 kb on + strand, within ECD_00925at 979.266 kb on + strand, within ECD_00925at 979.347 kb on + strandat 979.347 kb on + strandat 979.349 kb on + strandat 979.352 kb on + strandat 979.367 kb on + strandat 979.367 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas33
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977,634 + ECD_00923 0.89 +0.1
978,183 - ECD_00924 0.60 +1.2
978,516 + +0.5
978,516 + +0.4
978,518 + +0.1
978,518 + -0.1
978,518 + -0.8
978,519 - +0.8
978,538 + -0.1
978,538 + -0.6
978,541 + -0.5
978,541 + -0.9
978,541 + +0.1
978,919 + ECD_00925 0.37 -2.6
979,018 + ECD_00925 0.50 -0.1
979,266 + ECD_00925 0.81 -0.9
979,347 + +0.1
979,347 + +0.6
979,349 + -0.1
979,352 + -0.8
979,367 + -0.4
979,367 + +0.2

Or see this region's nucleotide sequence