Strain Fitness in Escherichia coli BL21 around ECD_00660

Experiment: Bas33

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00658 and ECD_00659 overlap by 1 nucleotidesECD_00659 and ECD_00660 are separated by 117 nucleotidesECD_00660 and ECD_00661 overlap by 4 nucleotidesECD_00661 and ECD_00662 are separated by 195 nucleotides ECD_00658: ECD_00658 - Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor, at 689,635 to 691,299 _00658 ECD_00659: ECD_00659 - putative membrane protein, at 691,299 to 691,625 _00659 ECD_00660: ECD_00660 - conserved protein, rhs-like protein, at 691,743 to 693,176 _00660 ECD_00661: ECD_00661 - putative secreted protein, at 693,173 to 693,742 _00661 ECD_00662: ECD_00662 - hypothetical protein, at 693,938 to 694,213 _00662 Position (kb) 691 692 693 694Strain fitness (log2 ratio) -1 0 1at 690.830 kb on + strand, within ECD_00658at 690.831 kb on - strand, within ECD_00658at 691.042 kb on - strand, within ECD_00658at 691.093 kb on - strand, within ECD_00658at 691.649 kb on + strandat 691.649 kb on + strandat 691.651 kb on + strandat 691.655 kb on + strandat 692.626 kb on - strand, within ECD_00660at 692.697 kb on + strand, within ECD_00660at 692.807 kb on - strand, within ECD_00660at 692.895 kb on + strand, within ECD_00660at 692.945 kb on + strand, within ECD_00660at 693.035 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas33
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690,830 + ECD_00658 0.72 +0.1
690,831 - ECD_00658 0.72 +0.2
691,042 - ECD_00658 0.85 -1.1
691,093 - ECD_00658 0.88 -0.4
691,649 + +0.1
691,649 + -0.6
691,651 + +1.1
691,655 + -0.0
692,626 - ECD_00660 0.62 -0.4
692,697 + ECD_00660 0.67 -0.9
692,807 - ECD_00660 0.74 +0.6
692,895 + ECD_00660 0.80 -1.1
692,945 + ECD_00660 0.84 +0.1
693,035 - +0.3

Or see this region's nucleotide sequence