Strain Fitness in Escherichia coli BL21 around ECD_00142

Experiment: Bas33

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00140 and ECD_00141 are separated by 369 nucleotidesECD_00141 and ECD_00142 overlap by 4 nucleotidesECD_00142 and ECD_00143 are separated by 92 nucleotides ECD_00140: ECD_00140 - putative fimbrial-like adhesin protein, at 159,151 to 159,735 _00140 ECD_00141: ECD_00141 - 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase, at 160,105 to 160,584 _00141 ECD_00142: ECD_00142 - poly(A) polymerase, at 160,581 to 161,945 _00142 ECD_00143: ECD_00143 - glutamyl-Q tRNA(Asp) synthetase, at 162,038 to 162,964 _00143 Position (kb) 160 161 162Strain fitness (log2 ratio) -1 0 1at 160.572 kb on - strandat 160.572 kb on - strandat 160.778 kb on - strand, within ECD_00142at 160.842 kb on - strand, within ECD_00142at 161.174 kb on - strand, within ECD_00142at 161.190 kb on - strand, within ECD_00142at 161.192 kb on - strand, within ECD_00142at 161.355 kb on - strand, within ECD_00142at 161.474 kb on - strand, within ECD_00142at 161.474 kb on - strand, within ECD_00142at 161.592 kb on - strand, within ECD_00142at 161.688 kb on - strand, within ECD_00142at 161.731 kb on - strand, within ECD_00142at 161.761 kb on - strand, within ECD_00142at 161.822 kb on - strandat 161.822 kb on - strandat 161.865 kb on - strandat 161.865 kb on - strandat 162.292 kb on - strand, within ECD_00143at 162.421 kb on - strand, within ECD_00143at 162.421 kb on - strand, within ECD_00143at 162.421 kb on - strand, within ECD_00143at 162.592 kb on - strand, within ECD_00143at 162.592 kb on - strand, within ECD_00143at 162.592 kb on - strand, within ECD_00143at 162.637 kb on - strand, within ECD_00143at 162.640 kb on - strand, within ECD_00143at 162.644 kb on + strand, within ECD_00143at 162.645 kb on - strand, within ECD_00143at 162.757 kb on + strand, within ECD_00143at 162.804 kb on - strand, within ECD_00143at 162.805 kb on + strand, within ECD_00143at 162.805 kb on + strand, within ECD_00143at 162.806 kb on - strand, within ECD_00143at 162.806 kb on - strand, within ECD_00143at 162.806 kb on - strand, within ECD_00143at 162.810 kb on - strand, within ECD_00143at 162.846 kb on + strand, within ECD_00143at 162.847 kb on - strand, within ECD_00143at 162.847 kb on - strand, within ECD_00143at 162.916 kb on + strandat 162.916 kb on + strandat 162.917 kb on - strandat 162.918 kb on + strandat 162.918 kb on + strandat 162.918 kb on + strandat 162.918 kb on + strandat 162.918 kb on + strandat 162.919 kb on - strandat 162.919 kb on - strandat 162.919 kb on - strandat 162.919 kb on - strandat 162.919 kb on - strandat 162.919 kb on - strandat 162.919 kb on - strandat 162.919 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas33
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160,572 - -0.0
160,572 - +0.8
160,778 - ECD_00142 0.14 -1.0
160,842 - ECD_00142 0.19 +0.2
161,174 - ECD_00142 0.43 -1.2
161,190 - ECD_00142 0.45 -0.5
161,192 - ECD_00142 0.45 -1.4
161,355 - ECD_00142 0.57 -1.4
161,474 - ECD_00142 0.65 +0.5
161,474 - ECD_00142 0.65 -1.0
161,592 - ECD_00142 0.74 -1.1
161,688 - ECD_00142 0.81 -0.7
161,731 - ECD_00142 0.84 -0.7
161,761 - ECD_00142 0.86 +1.2
161,822 - -0.0
161,822 - +0.1
161,865 - +0.2
161,865 - +0.5
162,292 - ECD_00143 0.27 +0.4
162,421 - ECD_00143 0.41 -0.1
162,421 - ECD_00143 0.41 -0.2
162,421 - ECD_00143 0.41 +0.9
162,592 - ECD_00143 0.60 +0.1
162,592 - ECD_00143 0.60 -0.8
162,592 - ECD_00143 0.60 -0.4
162,637 - ECD_00143 0.65 -0.5
162,640 - ECD_00143 0.65 +0.2
162,644 + ECD_00143 0.65 -0.2
162,645 - ECD_00143 0.65 +0.3
162,757 + ECD_00143 0.78 +0.4
162,804 - ECD_00143 0.83 +0.6
162,805 + ECD_00143 0.83 +0.1
162,805 + ECD_00143 0.83 +0.2
162,806 - ECD_00143 0.83 +0.2
162,806 - ECD_00143 0.83 +0.2
162,806 - ECD_00143 0.83 +0.0
162,810 - ECD_00143 0.83 +0.0
162,846 + ECD_00143 0.87 +0.5
162,847 - ECD_00143 0.87 -0.6
162,847 - ECD_00143 0.87 -0.5
162,916 + +0.5
162,916 + -0.2
162,917 - +0.0
162,918 + +0.2
162,918 + -0.2
162,918 + -0.3
162,918 + -0.2
162,918 + +0.1
162,919 - -1.0
162,919 - -0.2
162,919 - +0.5
162,919 - +0.2
162,919 - -0.8
162,919 - -1.6
162,919 - -0.2
162,919 - -1.2

Or see this region's nucleotide sequence