Strain Fitness in Escherichia coli BL21 around ECD_00001

Experiment: Bas33

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntECD_00001 and ECD_00002 are separated by 80 nucleotides ECD_00001: ECD_00001 - thr operon leader peptide, at 190 to 255 _00001 ECD_00002: ECD_00002 - Bifunctional aspartokinase/homoserine dehydrogenase 1, at 336 to 2,798 _00002 Position (kb) 0 1Strain fitness (log2 ratio) -1 0 1at 0.029 kb on + strandat 0.029 kb on + strandat 0.117 kb on + strandat 0.118 kb on - strandat 0.125 kb on + strandat 0.126 kb on - strandat 0.143 kb on + strandat 0.143 kb on + strandat 0.143 kb on + strandat 0.143 kb on + strandat 0.143 kb on + strandat 0.178 kb on + strandat 0.178 kb on + strandat 0.179 kb on - strandat 0.201 kb on + strand, within ECD_00001at 0.202 kb on - strand, within ECD_00001at 0.240 kb on + strand, within ECD_00001at 0.328 kb on + strandat 0.329 kb on - strandat 0.329 kb on - strandat 0.659 kb on + strand, within ECD_00002at 0.659 kb on + strand, within ECD_00002at 0.735 kb on - strand, within ECD_00002at 0.745 kb on + strand, within ECD_00002at 0.746 kb on - strand, within ECD_00002at 0.748 kb on + strand, within ECD_00002at 0.749 kb on - strand, within ECD_00002at 0.813 kb on + strand, within ECD_00002at 0.988 kb on + strand, within ECD_00002at 0.989 kb on - strand, within ECD_00002at 1.032 kb on + strand, within ECD_00002at 1.032 kb on + strand, within ECD_00002at 1.032 kb on + strand, within ECD_00002at 1.035 kb on - strand, within ECD_00002

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Bas33
remove
29 + +0.1
29 + +0.1
117 + +0.7
118 - +0.3
125 + -0.6
126 - +0.5
143 + +0.3
143 + -0.4
143 + +0.1
143 + -0.1
143 + -0.6
178 + -0.9
178 + -0.4
179 - +0.4
201 + ECD_00001 0.17 -0.6
202 - ECD_00001 0.18 -0.2
240 + ECD_00001 0.76 -0.6
328 + -0.2
329 - -0.2
329 - -0.0
659 + ECD_00002 0.13 -0.0
659 + ECD_00002 0.13 -0.4
735 - ECD_00002 0.16 -0.4
745 + ECD_00002 0.17 -0.4
746 - ECD_00002 0.17 -0.8
748 + ECD_00002 0.17 -0.9
749 - ECD_00002 0.17 -0.5
813 + ECD_00002 0.19 -0.5
988 + ECD_00002 0.26 -0.4
989 - ECD_00002 0.27 -0.0
1,032 + ECD_00002 0.28 -0.2
1,032 + ECD_00002 0.28 +0.7
1,032 + ECD_00002 0.28 +0.1
1,035 - ECD_00002 0.28 +0.1

Or see this region's nucleotide sequence