Strain Fitness in Escherichia coli BL21 around ECD_02404

Experiment: Ox4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02403 and ECD_02404 are separated by 117 nucleotidesECD_02404 and ECD_02405 are separated by 10 nucleotidesECD_02405 and ECD_02406 are separated by 17 nucleotides ECD_02403: ECD_02403 - GTPase; multicopy suppressor of ftsJ, at 2,500,364 to 2,501,836 _02403 ECD_02404: ECD_02404 - BamABCDE complex OM biogenesis lipoprotein, at 2,501,954 to 2,503,132 _02404 ECD_02405: ECD_02405 - ancillary SecYEG translocon subunit; putative anti-RcsB factor, at 2,503,143 to 2,503,763 _02405 ECD_02406: ECD_02406 - histidyl tRNA synthetase, at 2,503,781 to 2,505,055 _02406 Position (kb) 2501 2502 2503 2504Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2501.857 kb on - strandat 2501.879 kb on - strandat 2501.896 kb on - strandat 2501.963 kb on - strandat 2501.963 kb on - strandat 2501.963 kb on - strandat 2501.963 kb on - strandat 2502.136 kb on - strand, within ECD_02404at 2502.345 kb on + strand, within ECD_02404at 2502.346 kb on - strand, within ECD_02404at 2502.621 kb on - strand, within ECD_02404at 2502.766 kb on - strand, within ECD_02404at 2503.197 kb on - strandat 2503.230 kb on - strand, within ECD_02405at 2503.453 kb on - strand, within ECD_02405at 2503.508 kb on - strand, within ECD_02405at 2503.512 kb on - strand, within ECD_02405

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Per-strain Table

Position Strand Gene LocusTag Fraction Ox4
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2,501,857 - +0.2
2,501,879 - +0.3
2,501,896 - -0.1
2,501,963 - -1.1
2,501,963 - -1.3
2,501,963 - -1.4
2,501,963 - +0.2
2,502,136 - ECD_02404 0.15 +3.0
2,502,345 + ECD_02404 0.33 +0.9
2,502,346 - ECD_02404 0.33 +1.6
2,502,621 - ECD_02404 0.57 +2.5
2,502,766 - ECD_02404 0.69 +2.0
2,503,197 - +0.5
2,503,230 - ECD_02405 0.14 -0.5
2,503,453 - ECD_02405 0.50 -0.4
2,503,508 - ECD_02405 0.59 -2.2
2,503,512 - ECD_02405 0.59 -1.5

Or see this region's nucleotide sequence