Strain Fitness in Escherichia coli BL21 around ECD_01169

Experiment: Ox4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01167 and ECD_01168 are separated by 99 nucleotidesECD_01168 and ECD_01169 are separated by 1 nucleotidesECD_01169 and ECD_01170 are separated by 200 nucleotidesECD_01170 and ECD_01171 are separated by 177 nucleotides ECD_01167: ECD_01167 - murein tetrapeptide carboxypeptidase; LD-carboxypeptidase A, at 1,230,314 to 1,231,228 _01167 ECD_01168: ECD_01168 - lytic murein endotransglycosylase E, at 1,231,328 to 1,231,939 _01168 ECD_01169: ECD_01169 - flagellar velocity braking protein, c-di-GMP-regulated, at 1,231,941 to 1,232,675 _01169 ECD_01170: ECD_01170 - UPF0410 family putative inner membrane protein, at 1,232,876 to 1,233,130 _01170 ECD_01171: ECD_01171 - uncharacterized protein, at 1,233,308 to 1,233,748 _01171 Position (kb) 1231 1232 1233Strain fitness (log2 ratio) -1 0 1at 1231.186 kb on + strandat 1231.262 kb on - strandat 1231.262 kb on - strandat 1231.263 kb on + strandat 1231.313 kb on - strandat 1231.575 kb on + strand, within ECD_01168at 1231.763 kb on + strand, within ECD_01168at 1231.907 kb on + strandat 1231.994 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ox4
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1,231,186 + -1.5
1,231,262 - -0.1
1,231,262 - -1.4
1,231,263 + +0.3
1,231,313 - -0.1
1,231,575 + ECD_01168 0.40 +0.3
1,231,763 + ECD_01168 0.71 +0.2
1,231,907 + +0.1
1,231,994 + -0.8

Or see this region's nucleotide sequence