Strain Fitness in Escherichia coli BL21 around ECD_00191

Experiment: Ox4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00189 and ECD_00190 overlap by 4 nucleotidesECD_00190 and ECD_00191 are separated by 13 nucleotidesECD_00191 and ECD_00192 are separated by 199 nucleotides ECD_00189: ECD_00189 - PDDEXK superfamily protein, at 217,134 to 217,679 _00189 ECD_00190: ECD_00190 - alternative stalled-ribosome rescue factor B; peptidyl-tRNA hydrolase, ribosome-attached, at 217,676 to 218,098 _00190 ECD_00191: ECD_00191 - lipoprotein involved with copper homeostasis and adhesion, at 218,112 to 218,822 _00191 ECD_00192: ECD_00192 - putative lipoprotein, at 219,022 to 219,846 _00192 Position (kb) 218 219Strain fitness (log2 ratio) -2 -1 0 1 2at 217.250 kb on + strand, within ECD_00189at 217.251 kb on - strand, within ECD_00189at 217.281 kb on + strand, within ECD_00189at 217.281 kb on + strand, within ECD_00189at 217.452 kb on + strand, within ECD_00189at 217.452 kb on + strand, within ECD_00189at 217.452 kb on + strand, within ECD_00189at 217.452 kb on + strand, within ECD_00189at 217.453 kb on - strand, within ECD_00189at 217.453 kb on - strand, within ECD_00189at 217.454 kb on + strand, within ECD_00189at 217.454 kb on + strand, within ECD_00189at 217.638 kb on - strandat 217.769 kb on - strand, within ECD_00190at 217.826 kb on + strand, within ECD_00190at 217.827 kb on - strand, within ECD_00190at 217.829 kb on + strand, within ECD_00190at 217.829 kb on + strand, within ECD_00190at 217.968 kb on + strand, within ECD_00190at 217.968 kb on + strand, within ECD_00190at 218.159 kb on + strandat 218.159 kb on + strandat 218.174 kb on + strandat 218.177 kb on + strandat 218.373 kb on + strand, within ECD_00191at 218.378 kb on + strand, within ECD_00191at 218.508 kb on + strand, within ECD_00191at 218.509 kb on - strand, within ECD_00191at 218.511 kb on - strand, within ECD_00191at 218.536 kb on + strand, within ECD_00191at 218.536 kb on + strand, within ECD_00191at 218.537 kb on - strand, within ECD_00191at 218.540 kb on + strand, within ECD_00191at 218.568 kb on + strand, within ECD_00191at 218.574 kb on + strand, within ECD_00191at 218.575 kb on - strand, within ECD_00191at 218.576 kb on + strand, within ECD_00191at 218.695 kb on + strand, within ECD_00191at 218.704 kb on + strand, within ECD_00191at 218.718 kb on - strand, within ECD_00191at 218.759 kb on - strandat 218.969 kb on + strandat 218.970 kb on - strandat 218.978 kb on + strandat 218.978 kb on + strandat 218.993 kb on - strandat 219.015 kb on + strandat 219.016 kb on - strandat 219.059 kb on + strandat 219.059 kb on + strandat 219.060 kb on - strandat 219.086 kb on + strandat 219.087 kb on - strandat 219.135 kb on + strand, within ECD_00192at 219.135 kb on + strand, within ECD_00192at 219.136 kb on - strand, within ECD_00192at 219.180 kb on + strand, within ECD_00192at 219.381 kb on - strand, within ECD_00192at 219.384 kb on + strand, within ECD_00192at 219.394 kb on - strand, within ECD_00192at 219.480 kb on + strand, within ECD_00192at 219.743 kb on - strand, within ECD_00192at 219.774 kb on + strandat 219.778 kb on + strandat 219.778 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ox4
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217,250 + ECD_00189 0.21 -1.0
217,251 - ECD_00189 0.21 -0.5
217,281 + ECD_00189 0.27 +0.6
217,281 + ECD_00189 0.27 +0.3
217,452 + ECD_00189 0.58 -0.4
217,452 + ECD_00189 0.58 -0.7
217,452 + ECD_00189 0.58 -0.5
217,452 + ECD_00189 0.58 +2.5
217,453 - ECD_00189 0.58 -2.0
217,453 - ECD_00189 0.58 +0.2
217,454 + ECD_00189 0.59 +0.1
217,454 + ECD_00189 0.59 +0.2
217,638 - +0.7
217,769 - ECD_00190 0.22 +0.3
217,826 + ECD_00190 0.35 -1.1
217,827 - ECD_00190 0.36 -0.5
217,829 + ECD_00190 0.36 -1.2
217,829 + ECD_00190 0.36 +0.4
217,968 + ECD_00190 0.69 +0.1
217,968 + ECD_00190 0.69 +1.2
218,159 + -0.2
218,159 + -1.0
218,174 + +0.0
218,177 + +0.0
218,373 + ECD_00191 0.37 +0.8
218,378 + ECD_00191 0.37 +0.7
218,508 + ECD_00191 0.56 -0.6
218,509 - ECD_00191 0.56 -1.2
218,511 - ECD_00191 0.56 +1.4
218,536 + ECD_00191 0.60 +1.4
218,536 + ECD_00191 0.60 -0.1
218,537 - ECD_00191 0.60 +0.0
218,540 + ECD_00191 0.60 -0.4
218,568 + ECD_00191 0.64 +0.0
218,574 + ECD_00191 0.65 +0.9
218,575 - ECD_00191 0.65 +0.6
218,576 + ECD_00191 0.65 -0.2
218,695 + ECD_00191 0.82 -0.3
218,704 + ECD_00191 0.83 -0.1
218,718 - ECD_00191 0.85 +1.2
218,759 - +0.2
218,969 + -1.3
218,970 - +0.4
218,978 + -0.5
218,978 + +0.8
218,993 - +1.8
219,015 + +0.2
219,016 - +1.0
219,059 + +0.6
219,059 + +0.1
219,060 - -0.3
219,086 + -1.1
219,087 - -0.3
219,135 + ECD_00192 0.14 -1.4
219,135 + ECD_00192 0.14 +0.7
219,136 - ECD_00192 0.14 -1.2
219,180 + ECD_00192 0.19 -0.1
219,381 - ECD_00192 0.44 +0.8
219,384 + ECD_00192 0.44 +0.9
219,394 - ECD_00192 0.45 +1.3
219,480 + ECD_00192 0.56 +1.0
219,743 - ECD_00192 0.87 -0.3
219,774 + +0.8
219,778 + -0.7
219,778 + -0.4

Or see this region's nucleotide sequence