Strain Fitness in Escherichia coli BL21 around ECD_02116

Experiment: Ox4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02114 and ECD_02115 are separated by 124 nucleotidesECD_02115 and ECD_02116 are separated by 138 nucleotidesECD_02116 and ECD_02117 are separated by 181 nucleotidesECD_02117 and ECD_02118 are separated by 19 nucleotides ECD_02114: ECD_02114 - putative ATP-dependent DNA or RNA helicase, at 2,175,481 to 2,177,241 _02114 ECD_02115: ECD_02115 - 50S ribosomal subunit protein L25, at 2,177,366 to 2,177,650 _02115 ECD_02116: ECD_02116 - spermidine nucleoid-associated protein, at 2,177,789 to 2,178,796 _02116 ECD_02117: ECD_02117 - UPF0352 family protein, at 2,178,978 to 2,179,205 _02117 ECD_02118: ECD_02118 - essential inner membrane DUF3413 domain-containing protein; lipid A production and membrane permeability factor, at 2,179,225 to 2,180,985 _02118 Position (kb) 2177 2178 2179Strain fitness (log2 ratio) -3 -2 -1 0 1at 2176.860 kb on + strand, within ECD_02114at 2176.860 kb on + strand, within ECD_02114at 2176.969 kb on + strand, within ECD_02114at 2177.246 kb on - strandat 2177.895 kb on - strand, within ECD_02116at 2177.915 kb on - strand, within ECD_02116at 2178.111 kb on + strand, within ECD_02116at 2178.112 kb on - strand, within ECD_02116at 2178.115 kb on + strand, within ECD_02116at 2178.116 kb on - strand, within ECD_02116at 2178.116 kb on - strand, within ECD_02116at 2178.116 kb on - strand, within ECD_02116at 2178.116 kb on - strand, within ECD_02116at 2178.118 kb on - strand, within ECD_02116at 2178.118 kb on - strand, within ECD_02116at 2178.591 kb on - strand, within ECD_02116at 2178.758 kb on - strandat 2178.800 kb on - strandat 2178.811 kb on + strandat 2178.812 kb on - strandat 2178.855 kb on - strandat 2178.942 kb on + strandat 2178.977 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ox4
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2,176,860 + ECD_02114 0.78 -0.0
2,176,860 + ECD_02114 0.78 -0.8
2,176,969 + ECD_02114 0.84 +1.0
2,177,246 - +0.9
2,177,895 - ECD_02116 0.11 -3.0
2,177,915 - ECD_02116 0.12 -0.6
2,178,111 + ECD_02116 0.32 -0.3
2,178,112 - ECD_02116 0.32 -1.2
2,178,115 + ECD_02116 0.32 -0.8
2,178,116 - ECD_02116 0.32 -1.3
2,178,116 - ECD_02116 0.32 -1.8
2,178,116 - ECD_02116 0.32 -0.2
2,178,116 - ECD_02116 0.32 -0.2
2,178,118 - ECD_02116 0.33 +0.2
2,178,118 - ECD_02116 0.33 -0.8
2,178,591 - ECD_02116 0.80 -1.2
2,178,758 - -0.4
2,178,800 - -1.9
2,178,811 + -1.5
2,178,812 - -0.7
2,178,855 - +0.7
2,178,942 + -0.3
2,178,977 + -1.2

Or see this region's nucleotide sequence