Strain Fitness in Escherichia coli BL21 around ECD_01216

Experiment: Ox4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01215 and ECD_01216 are separated by 476 nucleotidesECD_01216 and ECD_01217 are separated by 737 nucleotides ECD_01215: ECD_01215 - fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase, at 1,282,237 to 1,284,912 _01215 ECD_01216: ECD_01216 - UPF0056 family inner membrane protein, at 1,285,389 to 1,286,036 _01216 ECD_01217: ECD_01217 - oligopeptide ABC transporter periplasmic binding protein, at 1,286,774 to 1,288,405 _01217 Position (kb) 1285 1286 1287Strain fitness (log2 ratio) -3 -2 -1 0 1at 1284.553 kb on - strand, within ECD_01215at 1284.553 kb on - strand, within ECD_01215at 1284.684 kb on - strandat 1284.690 kb on - strandat 1285.557 kb on - strand, within ECD_01216at 1285.607 kb on + strand, within ECD_01216at 1285.694 kb on + strand, within ECD_01216at 1285.829 kb on + strand, within ECD_01216at 1285.991 kb on + strandat 1286.610 kb on + strandat 1286.610 kb on + strandat 1286.610 kb on + strandat 1286.658 kb on + strandat 1286.658 kb on + strandat 1286.658 kb on + strandat 1286.658 kb on + strandat 1286.658 kb on + strandat 1286.658 kb on + strandat 1286.750 kb on + strandat 1286.802 kb on + strandat 1286.859 kb on + strandat 1286.859 kb on + strandat 1286.962 kb on + strand, within ECD_01217at 1286.962 kb on + strand, within ECD_01217at 1286.965 kb on + strand, within ECD_01217

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Per-strain Table

Position Strand Gene LocusTag Fraction Ox4
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1,284,553 - ECD_01215 0.87 +0.3
1,284,553 - ECD_01215 0.87 -0.8
1,284,684 - -0.2
1,284,690 - +0.1
1,285,557 - ECD_01216 0.26 +0.2
1,285,607 + ECD_01216 0.34 +1.0
1,285,694 + ECD_01216 0.47 +0.8
1,285,829 + ECD_01216 0.68 +0.8
1,285,991 + +0.7
1,286,610 + -0.2
1,286,610 + -0.3
1,286,610 + -2.4
1,286,658 + +1.4
1,286,658 + -0.1
1,286,658 + +0.2
1,286,658 + -0.4
1,286,658 + +0.6
1,286,658 + -0.4
1,286,750 + +1.2
1,286,802 + -1.0
1,286,859 + +0.1
1,286,859 + -0.1
1,286,962 + ECD_01217 0.12 -2.8
1,286,962 + ECD_01217 0.12 +1.4
1,286,965 + ECD_01217 0.12 -0.7

Or see this region's nucleotide sequence