Strain Fitness in Escherichia coli BL21 around ECD_00020

Experiment: Ox4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00019 and ECD_00020 are separated by 38 nucleotidesECD_00020 and ECD_00021 are separated by 12 nucleotides ECD_00019: ECD_00019 - transcriptional activator of nhaA, at 18,714 to 19,619 _00019 ECD_00020: ECD_00020 - hypothetical protein, at 19,658 to 20,617 _00020 ECD_00021: ECD_00021 - putative usher protein, at 20,630 to 21,775 _00021 Position (kb) 19 20 21Strain fitness (log2 ratio) -2 -1 0 1at 18.728 kb on + strandat 18.736 kb on - strandat 18.751 kb on - strandat 18.765 kb on + strandat 18.765 kb on + strandat 18.805 kb on - strand, within ECD_00019at 18.868 kb on + strand, within ECD_00019at 18.872 kb on + strand, within ECD_00019at 18.978 kb on + strand, within ECD_00019at 18.978 kb on + strand, within ECD_00019at 18.997 kb on + strand, within ECD_00019at 18.998 kb on - strand, within ECD_00019at 19.051 kb on + strand, within ECD_00019at 19.051 kb on + strand, within ECD_00019at 19.051 kb on + strand, within ECD_00019at 19.051 kb on + strand, within ECD_00019at 19.069 kb on + strand, within ECD_00019at 19.132 kb on + strand, within ECD_00019at 19.181 kb on + strand, within ECD_00019at 19.182 kb on - strand, within ECD_00019at 19.458 kb on + strand, within ECD_00019at 19.654 kb on + strandat 19.660 kb on - strandat 19.660 kb on - strandat 19.715 kb on - strandat 19.722 kb on + strandat 19.959 kb on - strand, within ECD_00020at 20.517 kb on - strand, within ECD_00020at 20.852 kb on + strand, within ECD_00021at 20.853 kb on - strand, within ECD_00021at 21.201 kb on + strand, within ECD_00021at 21.213 kb on - strand, within ECD_00021

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Per-strain Table

Position Strand Gene LocusTag Fraction Ox4
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18,728 + +0.3
18,736 - -0.5
18,751 - +1.1
18,765 + +0.8
18,765 + +1.1
18,805 - ECD_00019 0.10 -0.4
18,868 + ECD_00019 0.17 +0.3
18,872 + ECD_00019 0.17 -0.4
18,978 + ECD_00019 0.29 +0.2
18,978 + ECD_00019 0.29 -0.9
18,997 + ECD_00019 0.31 -0.6
18,998 - ECD_00019 0.31 +0.2
19,051 + ECD_00019 0.37 +0.0
19,051 + ECD_00019 0.37 +0.6
19,051 + ECD_00019 0.37 +0.3
19,051 + ECD_00019 0.37 -0.7
19,069 + ECD_00019 0.39 -1.0
19,132 + ECD_00019 0.46 +0.9
19,181 + ECD_00019 0.52 -0.0
19,182 - ECD_00019 0.52 +0.3
19,458 + ECD_00019 0.82 -0.4
19,654 + -0.5
19,660 - +0.4
19,660 - -0.8
19,715 - +0.2
19,722 + -2.4
19,959 - ECD_00020 0.31 +0.7
20,517 - ECD_00020 0.89 -0.7
20,852 + ECD_00021 0.19 +1.0
20,853 - ECD_00021 0.19 +1.0
21,201 + ECD_00021 0.50 +0.3
21,213 - ECD_00021 0.51 -0.1

Or see this region's nucleotide sequence