Strain Fitness in Escherichia coli BL21 around ECD_01717

Experiment: Bas32

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01716 and ECD_01717 overlap by 4 nucleotidesECD_01717 and ECD_01718 are separated by 445 nucleotides ECD_01716: ECD_01716 - arginine succinyltransferase, at 1,774,144 to 1,775,178 _01716 ECD_01717: ECD_01717 - succinylornithine transaminase, PLP-dependent, at 1,775,175 to 1,776,395 _01717 ECD_01718: ECD_01718 - exonuclease III, at 1,776,841 to 1,777,647 _01718 Position (kb) 1775 1776 1777Strain fitness (log2 ratio) -2 -1 0 1at 1774.433 kb on + strand, within ECD_01716at 1774.433 kb on + strand, within ECD_01716at 1774.434 kb on - strand, within ECD_01716at 1774.434 kb on - strand, within ECD_01716at 1775.357 kb on - strand, within ECD_01717at 1775.371 kb on - strand, within ECD_01717at 1775.430 kb on - strand, within ECD_01717at 1775.551 kb on + strand, within ECD_01717at 1775.649 kb on - strand, within ECD_01717at 1775.652 kb on + strand, within ECD_01717at 1775.690 kb on - strand, within ECD_01717at 1775.847 kb on - strand, within ECD_01717at 1775.880 kb on - strand, within ECD_01717at 1775.886 kb on - strand, within ECD_01717at 1775.888 kb on - strand, within ECD_01717at 1775.935 kb on + strand, within ECD_01717at 1776.189 kb on - strand, within ECD_01717at 1776.275 kb on - strandat 1776.275 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas32
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1,774,433 + ECD_01716 0.28 -0.7
1,774,433 + ECD_01716 0.28 -1.8
1,774,434 - ECD_01716 0.28 -0.8
1,774,434 - ECD_01716 0.28 +0.3
1,775,357 - ECD_01717 0.15 +0.3
1,775,371 - ECD_01717 0.16 +0.0
1,775,430 - ECD_01717 0.21 +0.5
1,775,551 + ECD_01717 0.31 +1.5
1,775,649 - ECD_01717 0.39 -0.6
1,775,652 + ECD_01717 0.39 +0.5
1,775,690 - ECD_01717 0.42 +0.4
1,775,847 - ECD_01717 0.55 -0.4
1,775,880 - ECD_01717 0.58 +0.5
1,775,886 - ECD_01717 0.58 +0.8
1,775,888 - ECD_01717 0.58 -0.4
1,775,935 + ECD_01717 0.62 +0.6
1,776,189 - ECD_01717 0.83 +1.5
1,776,275 - +0.8
1,776,275 - -1.5

Or see this region's nucleotide sequence