Strain Fitness in Escherichia coli BL21 around ECD_02148

Experiment: Bas26

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02147 and ECD_02148 are separated by 195 nucleotidesECD_02148 and ECD_02149 overlap by 1 nucleotidesECD_02149 and ECD_02150 overlap by 4 nucleotides ECD_02147: ECD_02147 - fused response regulator of ato operon, in two-component system with AtoS: response regulator/sigma54 interaction protein, at 2,212,227 to 2,213,612 _02147 ECD_02148: ECD_02148 - acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit, at 2,213,808 to 2,214,470 _02148 ECD_02149: ECD_02149 - acetyl-CoA:acetoacetyl-CoA transferase, beta subunit, at 2,214,470 to 2,215,120 _02149 ECD_02150: ECD_02150 - short chain fatty acid transporter, at 2,215,117 to 2,216,439 _02150 Position (kb) 2213 2214 2215Strain fitness (log2 ratio) -1 0 1at 2212.996 kb on + strand, within ECD_02147at 2213.104 kb on + strand, within ECD_02147at 2213.158 kb on + strand, within ECD_02147at 2213.193 kb on - strand, within ECD_02147at 2213.572 kb on - strandat 2213.608 kb on + strandat 2213.609 kb on - strandat 2213.763 kb on - strandat 2213.830 kb on + strandat 2213.830 kb on + strandat 2213.886 kb on - strand, within ECD_02148at 2214.074 kb on - strand, within ECD_02148at 2214.111 kb on - strand, within ECD_02148at 2214.307 kb on - strand, within ECD_02148at 2214.356 kb on + strand, within ECD_02148at 2214.589 kb on + strand, within ECD_02149at 2214.590 kb on - strand, within ECD_02149at 2214.590 kb on - strand, within ECD_02149at 2214.997 kb on + strand, within ECD_02149at 2215.099 kb on + strandat 2215.116 kb on + strandat 2215.117 kb on - strandat 2215.314 kb on + strand, within ECD_02150

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas26
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2,212,996 + ECD_02147 0.55 +1.7
2,213,104 + ECD_02147 0.63 -0.6
2,213,158 + ECD_02147 0.67 +1.7
2,213,193 - ECD_02147 0.70 +0.3
2,213,572 - -0.5
2,213,608 + +0.2
2,213,609 - -0.0
2,213,763 - -0.5
2,213,830 + +1.2
2,213,830 + -0.4
2,213,886 - ECD_02148 0.12 +0.4
2,214,074 - ECD_02148 0.40 +0.3
2,214,111 - ECD_02148 0.46 +0.6
2,214,307 - ECD_02148 0.75 +1.0
2,214,356 + ECD_02148 0.83 -1.3
2,214,589 + ECD_02149 0.18 +0.3
2,214,590 - ECD_02149 0.18 +1.4
2,214,590 - ECD_02149 0.18 -0.2
2,214,997 + ECD_02149 0.81 +0.8
2,215,099 + +0.4
2,215,116 + +0.3
2,215,117 - +0.1
2,215,314 + ECD_02150 0.15 -0.2

Or see this region's nucleotide sequence