Strain Fitness in Escherichia coli BL21 around ECD_02329

Experiment: Bas24

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02328 and ECD_02329 are separated by 3 nucleotidesECD_02329 and ECD_02330 overlap by 83 nucleotidesECD_02330 and ECD_02331 are separated by 85 nucleotides ECD_02328: ECD_02328 - N-acetylmuramic acid 6-phosphate (MurNAc-6-P) etherase, at 2,418,176 to 2,419,072 _02328 ECD_02329: ECD_02329 - N-acetylmuramic acid permease, EIIBC component, PTS system, at 2,419,076 to 2,420,500 _02329 ECD_02330: ECD_02330 - penicillin binding protein PBP4B; weak DD-carboxypeptidase activity, at 2,420,418 to 2,420,594 _02330 ECD_02331: ECD_02331 - porphyrinogen oxidase, cytoplasmic, at 2,420,680 to 2,421,579 _02331 Position (kb) 2419 2420 2421Strain fitness (log2 ratio) -2 -1 0 1at 2418.159 kb on + strandat 2418.159 kb on + strandat 2418.160 kb on - strandat 2418.162 kb on + strandat 2418.163 kb on - strandat 2418.277 kb on + strand, within ECD_02328at 2418.374 kb on + strand, within ECD_02328at 2418.726 kb on + strand, within ECD_02328at 2418.895 kb on + strand, within ECD_02328at 2418.945 kb on + strand, within ECD_02328at 2419.117 kb on + strandat 2419.163 kb on - strandat 2419.163 kb on - strandat 2419.185 kb on + strandat 2419.185 kb on + strandat 2419.186 kb on - strandat 2419.495 kb on - strand, within ECD_02329at 2419.558 kb on + strand, within ECD_02329at 2419.611 kb on - strand, within ECD_02329at 2419.684 kb on + strand, within ECD_02329at 2419.759 kb on + strand, within ECD_02329at 2419.759 kb on + strand, within ECD_02329at 2419.760 kb on - strand, within ECD_02329at 2419.889 kb on + strand, within ECD_02329at 2419.900 kb on + strand, within ECD_02329at 2419.901 kb on - strand, within ECD_02329at 2419.930 kb on + strand, within ECD_02329at 2419.930 kb on + strand, within ECD_02329at 2419.930 kb on + strand, within ECD_02329at 2419.930 kb on + strand, within ECD_02329at 2419.930 kb on + strand, within ECD_02329at 2419.931 kb on - strand, within ECD_02329at 2419.931 kb on - strand, within ECD_02329at 2419.946 kb on - strand, within ECD_02329at 2419.961 kb on - strand, within ECD_02329at 2420.079 kb on - strand, within ECD_02329at 2420.080 kb on - strand, within ECD_02329at 2420.135 kb on + strand, within ECD_02329at 2420.257 kb on + strand, within ECD_02329at 2420.334 kb on + strand, within ECD_02329at 2420.455 kb on + strandat 2420.502 kb on - strand, within ECD_02330at 2420.523 kb on + strand, within ECD_02330at 2420.543 kb on + strand, within ECD_02330at 2420.543 kb on + strand, within ECD_02330at 2420.543 kb on + strand, within ECD_02330at 2420.588 kb on + strandat 2420.588 kb on + strandat 2420.588 kb on + strandat 2420.589 kb on - strandat 2420.589 kb on - strandat 2420.600 kb on + strandat 2420.600 kb on + strandat 2420.601 kb on - strandat 2420.602 kb on + strandat 2420.602 kb on + strandat 2420.602 kb on + strandat 2420.603 kb on - strandat 2420.623 kb on + strandat 2420.717 kb on + strandat 2420.766 kb on + strandat 2420.841 kb on - strand, within ECD_02331at 2420.841 kb on - strand, within ECD_02331at 2421.103 kb on - strand, within ECD_02331at 2421.331 kb on - strand, within ECD_02331at 2421.477 kb on + strand, within ECD_02331

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas24
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2,418,159 + +0.5
2,418,159 + -0.1
2,418,160 - -0.1
2,418,162 + -0.9
2,418,163 - +0.4
2,418,277 + ECD_02328 0.11 -0.7
2,418,374 + ECD_02328 0.22 +0.1
2,418,726 + ECD_02328 0.61 -0.6
2,418,895 + ECD_02328 0.80 +0.2
2,418,945 + ECD_02328 0.86 -1.1
2,419,117 + -1.8
2,419,163 - -1.3
2,419,163 - +0.4
2,419,185 + +1.1
2,419,185 + -0.6
2,419,186 - -1.6
2,419,495 - ECD_02329 0.29 +0.0
2,419,558 + ECD_02329 0.34 -0.8
2,419,611 - ECD_02329 0.38 -1.5
2,419,684 + ECD_02329 0.43 +0.2
2,419,759 + ECD_02329 0.48 -0.9
2,419,759 + ECD_02329 0.48 -2.6
2,419,760 - ECD_02329 0.48 -1.9
2,419,889 + ECD_02329 0.57 +0.6
2,419,900 + ECD_02329 0.58 -0.8
2,419,901 - ECD_02329 0.58 +0.8
2,419,930 + ECD_02329 0.60 -0.3
2,419,930 + ECD_02329 0.60 +0.4
2,419,930 + ECD_02329 0.60 +0.5
2,419,930 + ECD_02329 0.60 +0.6
2,419,930 + ECD_02329 0.60 +0.6
2,419,931 - ECD_02329 0.60 -0.9
2,419,931 - ECD_02329 0.60 -1.2
2,419,946 - ECD_02329 0.61 -0.8
2,419,961 - ECD_02329 0.62 -0.3
2,420,079 - ECD_02329 0.70 +0.3
2,420,080 - ECD_02329 0.70 -0.3
2,420,135 + ECD_02329 0.74 -0.6
2,420,257 + ECD_02329 0.83 -0.8
2,420,334 + ECD_02329 0.88 -0.5
2,420,455 + -0.1
2,420,502 - ECD_02330 0.47 +0.6
2,420,523 + ECD_02330 0.59 +1.5
2,420,543 + ECD_02330 0.71 -0.6
2,420,543 + ECD_02330 0.71 -0.8
2,420,543 + ECD_02330 0.71 +0.7
2,420,588 + +1.0
2,420,588 + -0.4
2,420,588 + -0.5
2,420,589 - -0.9
2,420,589 - -1.2
2,420,600 + -1.6
2,420,600 + +0.2
2,420,601 - +1.0
2,420,602 + +0.2
2,420,602 + +0.4
2,420,602 + -0.7
2,420,603 - +0.1
2,420,623 + -0.5
2,420,717 + +0.1
2,420,766 + -0.2
2,420,841 - ECD_02331 0.18 -2.4
2,420,841 - ECD_02331 0.18 -0.7
2,421,103 - ECD_02331 0.47 -1.9
2,421,331 - ECD_02331 0.72 +0.1
2,421,477 + ECD_02331 0.89 -2.5

Or see this region's nucleotide sequence