Strain Fitness in Escherichia coli BL21 around ECD_01789

Experiment: Bas24

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01787 and ECD_01788 are separated by 62 nucleotidesECD_01788 and ECD_01789 are separated by 12 nucleotidesECD_01789 and ECD_01790 are separated by 54 nucleotidesECD_01790 and ECD_01791 are separated by 428 nucleotides ECD_01787: ECD_01787 - fused mannose-specific PTS enzymes: IIA component/IIB component, at 1,847,775 to 1,848,746 _01787 ECD_01788: ECD_01788 - mannose-specific enzyme IIC component of PTS, at 1,848,809 to 1,849,609 _01788 ECD_01789: ECD_01789 - mannose-specific enzyme IID component of PTS, at 1,849,622 to 1,850,473 _01789 ECD_01790: ECD_01790 - UPF0266 family inner membrane protein, at 1,850,528 to 1,850,986 _01790 ECD_01791: ECD_01791 - putative Mn(2+) efflux pump, mntR-regulated, at 1,851,415 to 1,851,981 _01791 Position (kb) 1849 1850 1851Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1848.749 kb on + strandat 1848.749 kb on + strandat 1848.844 kb on + strandat 1849.150 kb on + strand, within ECD_01788at 1849.541 kb on + strandat 1849.630 kb on + strandat 1849.729 kb on + strand, within ECD_01789at 1849.729 kb on + strand, within ECD_01789at 1849.975 kb on - strand, within ECD_01789at 1850.123 kb on + strand, within ECD_01789at 1850.374 kb on + strand, within ECD_01789at 1851.240 kb on + strandat 1851.296 kb on + strandat 1851.356 kb on + strandat 1851.356 kb on + strandat 1851.357 kb on - strandat 1851.388 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas24
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1,848,749 + -0.8
1,848,749 + -1.1
1,848,844 + +0.2
1,849,150 + ECD_01788 0.43 -1.7
1,849,541 + +0.3
1,849,630 + -0.8
1,849,729 + ECD_01789 0.13 -0.8
1,849,729 + ECD_01789 0.13 -1.8
1,849,975 - ECD_01789 0.41 -1.9
1,850,123 + ECD_01789 0.59 -1.7
1,850,374 + ECD_01789 0.88 -3.8
1,851,240 + +0.5
1,851,296 + -2.5
1,851,356 + -1.2
1,851,356 + -0.6
1,851,357 - -2.4
1,851,388 + +1.3

Or see this region's nucleotide sequence