Strain Fitness in Escherichia coli BL21 around ECD_01321

Experiment: Bas24

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01320 and ECD_01321 are separated by 128 nucleotidesECD_01321 and ECD_01322 are separated by 51 nucleotides ECD_01320: ECD_01320 - ATP-dependent RNA helicase, specific for 23S rRNA, at 1,394,679 to 1,396,052 _01320 ECD_01321: ECD_01321 - tRNA s(2)C32 thioltransferase, iron sulfur cluster protein, at 1,396,181 to 1,397,116 _01321 ECD_01322: ECD_01322 - Rac prophage; integrase, at 1,397,168 to 1,398,403 _01322 Position (kb) 1396 1397 1398Strain fitness (log2 ratio) -1 0 1 2at 1395.385 kb on + strand, within ECD_01320at 1395.791 kb on + strand, within ECD_01320at 1396.607 kb on - strand, within ECD_01321at 1396.607 kb on - strand, within ECD_01321at 1396.960 kb on - strand, within ECD_01321at 1397.015 kb on - strand, within ECD_01321at 1397.015 kb on - strand, within ECD_01321at 1397.282 kb on - strandat 1397.327 kb on - strand, within ECD_01322at 1397.416 kb on - strand, within ECD_01322at 1397.542 kb on - strand, within ECD_01322at 1397.631 kb on - strand, within ECD_01322at 1397.914 kb on - strand, within ECD_01322

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas24
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1,395,385 + ECD_01320 0.51 -0.2
1,395,791 + ECD_01320 0.81 +1.3
1,396,607 - ECD_01321 0.46 +0.5
1,396,607 - ECD_01321 0.46 -0.4
1,396,960 - ECD_01321 0.83 -1.2
1,397,015 - ECD_01321 0.89 -1.2
1,397,015 - ECD_01321 0.89 -0.2
1,397,282 - +1.9
1,397,327 - ECD_01322 0.13 -0.8
1,397,416 - ECD_01322 0.20 -1.0
1,397,542 - ECD_01322 0.30 +0.1
1,397,631 - ECD_01322 0.37 -0.7
1,397,914 - ECD_01322 0.60 +0.3

Or see this region's nucleotide sequence