Strain Fitness in Escherichia coli BL21 around ECD_01227

Experiment: Bas24

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01225 and ECD_01226 are separated by 223 nucleotidesECD_01226 and ECD_01227 are separated by 39 nucleotidesECD_01227 and ECD_01228 are separated by 104 nucleotidesECD_01228 and ECD_01229 are separated by 29 nucleotides ECD_01225: ECD_01225 - putative DGPF domain-containing enzyme, at 1,296,161 to 1,296,457 _01225 ECD_01226: ECD_01226 - membrane spanning protein in TonB-ExbB-ExbD transport complex, at 1,296,681 to 1,297,400 _01226 ECD_01227: ECD_01227 - acyl-CoA esterase, at 1,297,440 to 1,297,838 _01227 ECD_01228: ECD_01228 - IspA family inner membrane protein, at 1,297,943 to 1,298,482 _01228 ECD_01229: ECD_01229 - UPF0259 family inner membrane protein, at 1,298,512 to 1,299,255 _01229 Position (kb) 1297 1298Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1296.492 kb on - strandat 1296.765 kb on + strand, within ECD_01226at 1296.767 kb on + strand, within ECD_01226at 1296.886 kb on + strand, within ECD_01226at 1297.214 kb on + strand, within ECD_01226at 1297.490 kb on - strand, within ECD_01227at 1297.494 kb on - strand, within ECD_01227at 1297.494 kb on - strand, within ECD_01227at 1297.516 kb on + strand, within ECD_01227at 1297.823 kb on + strandat 1297.824 kb on - strandat 1297.824 kb on - strandat 1297.842 kb on - strandat 1297.878 kb on + strandat 1298.530 kb on - strandat 1298.719 kb on - strand, within ECD_01229

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas24
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1,296,492 - +1.6
1,296,765 + ECD_01226 0.12 +1.0
1,296,767 + ECD_01226 0.12 -0.2
1,296,886 + ECD_01226 0.28 +0.7
1,297,214 + ECD_01226 0.74 +2.4
1,297,490 - ECD_01227 0.13 +0.3
1,297,494 - ECD_01227 0.14 -2.1
1,297,494 - ECD_01227 0.14 -1.4
1,297,516 + ECD_01227 0.19 +0.6
1,297,823 + -0.7
1,297,824 - -3.5
1,297,824 - +0.2
1,297,842 - +0.8
1,297,878 + +1.1
1,298,530 - -0.1
1,298,719 - ECD_01229 0.28 -0.1

Or see this region's nucleotide sequence