Strain Fitness in Escherichia coli BL21 around ECD_03041

Experiment: EV116

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_t00058 and ECD_03040 are separated by 14 nucleotidesECD_03040 and ECD_03041 are separated by 227 nucleotidesECD_03041 and ECD_03042 overlap by 8 nucleotidesECD_03042 and ECD_03043 are separated by 89 nucleotides ECD_t00058: ECD_t00058 - tRNA-Leu, at 3,190,571 to 3,190,657 _t00058 ECD_03040: ECD_03040 - preprotein translocase membrane subunit, at 3,190,672 to 3,191,004 _03040 ECD_03041: ECD_03041 - phosphoglucosamine mutase, at 3,191,232 to 3,192,569 _03041 ECD_03042: ECD_03042 - 7,8-dihydropteroate synthase, at 3,192,562 to 3,193,410 _03042 ECD_03043: ECD_03043 - protease, ATP-dependent zinc-metallo, at 3,193,500 to 3,195,434 _03043 Position (kb) 3191 3192 3193Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3190.261 kb on + strandat 3190.358 kb on - strandat 3190.404 kb on + strandat 3190.427 kb on + strandat 3190.433 kb on + strandat 3190.433 kb on + strandat 3190.494 kb on - strandat 3190.494 kb on - strandat 3190.667 kb on - strandat 3190.667 kb on - strandat 3190.783 kb on - strand, within ECD_03040at 3190.930 kb on - strand, within ECD_03040at 3190.975 kb on - strandat 3190.975 kb on - strandat 3190.988 kb on - strandat 3191.001 kb on - strandat 3191.005 kb on - strandat 3191.005 kb on - strandat 3191.036 kb on - strandat 3191.222 kb on - strandat 3191.226 kb on - strandat 3191.245 kb on - strandat 3193.436 kb on - strandat 3193.479 kb on - strandat 3193.502 kb on - strandat 3193.502 kb on - strandat 3193.502 kb on - strandat 3193.502 kb on - strandat 3193.502 kb on - strandat 3193.513 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV116
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3,190,261 + +0.5
3,190,358 - +1.3
3,190,404 + +0.1
3,190,427 + +0.9
3,190,433 + +1.0
3,190,433 + -1.6
3,190,494 - -1.7
3,190,494 - -0.5
3,190,667 - -0.3
3,190,667 - -0.3
3,190,783 - ECD_03040 0.33 -1.5
3,190,930 - ECD_03040 0.77 -4.1
3,190,975 - +0.6
3,190,975 - -2.3
3,190,988 - -0.3
3,191,001 - -0.1
3,191,005 - -1.0
3,191,005 - -2.2
3,191,036 - -0.8
3,191,222 - +2.3
3,191,226 - +1.1
3,191,245 - -0.2
3,193,436 - -0.8
3,193,479 - +1.0
3,193,502 - +2.2
3,193,502 - +1.7
3,193,502 - -0.1
3,193,502 - -2.8
3,193,502 - +2.1
3,193,513 - +2.4

Or see this region's nucleotide sequence