Strain Fitness in Escherichia coli BL21 around ECD_02131

Experiment: EV116

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02129 and ECD_02130 are separated by 9 nucleotidesECD_02130 and ECD_02131 overlap by 4 nucleotidesECD_02131 and ECD_02132 overlap by 14 nucleotidesECD_02132 and ECD_02133 are separated by 6 nucleotides ECD_02129: ECD_02129 - quinol dehydrogenase, electron source for NapAB, at 2,191,307 to 2,191,909 _02129 ECD_02130: ECD_02130 - nitrate reductase, small, cytochrome C550 subunit, periplasmic, at 2,191,919 to 2,192,368 _02130 ECD_02131: ECD_02131 - ferredoxin-type protein, at 2,192,365 to 2,193,228 _02131 ECD_02132: ECD_02132 - ferredoxin-type protein, at 2,193,215 to 2,193,910 _02132 ECD_02133: ECD_02133 - nitrate reductase, periplasmic, large subunit, at 2,193,917 to 2,196,403 _02133 Position (kb) 2192 2193 2194Strain fitness (log2 ratio) -2 -1 0 1 2at 2191.372 kb on + strand, within ECD_02129at 2191.444 kb on + strand, within ECD_02129at 2191.578 kb on + strand, within ECD_02129at 2191.578 kb on + strand, within ECD_02129at 2191.578 kb on + strand, within ECD_02129at 2191.579 kb on - strand, within ECD_02129at 2191.680 kb on + strand, within ECD_02129at 2191.681 kb on - strand, within ECD_02129at 2191.698 kb on + strand, within ECD_02129at 2191.735 kb on + strand, within ECD_02129at 2191.736 kb on - strand, within ECD_02129at 2191.891 kb on + strandat 2191.892 kb on - strandat 2191.913 kb on - strandat 2192.132 kb on - strand, within ECD_02130at 2192.190 kb on + strand, within ECD_02130at 2192.395 kb on - strandat 2192.403 kb on + strandat 2192.403 kb on + strandat 2192.404 kb on - strandat 2192.404 kb on - strandat 2192.408 kb on - strandat 2192.408 kb on - strandat 2192.408 kb on - strandat 2192.408 kb on - strandat 2192.504 kb on - strand, within ECD_02131at 2192.504 kb on - strand, within ECD_02131at 2192.610 kb on - strand, within ECD_02131at 2192.663 kb on - strand, within ECD_02131at 2192.689 kb on - strand, within ECD_02131at 2192.777 kb on - strand, within ECD_02131at 2192.879 kb on - strand, within ECD_02131at 2193.069 kb on - strand, within ECD_02131at 2193.266 kb on + strandat 2193.266 kb on + strandat 2193.267 kb on - strandat 2193.340 kb on + strand, within ECD_02132at 2193.693 kb on + strand, within ECD_02132at 2194.033 kb on - strandat 2194.033 kb on - strandat 2194.033 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV116
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2,191,372 + ECD_02129 0.11 +0.2
2,191,444 + ECD_02129 0.23 -1.6
2,191,578 + ECD_02129 0.45 -0.7
2,191,578 + ECD_02129 0.45 -0.0
2,191,578 + ECD_02129 0.45 +1.4
2,191,579 - ECD_02129 0.45 -0.1
2,191,680 + ECD_02129 0.62 -1.0
2,191,681 - ECD_02129 0.62 +0.2
2,191,698 + ECD_02129 0.65 +0.4
2,191,735 + ECD_02129 0.71 +1.0
2,191,736 - ECD_02129 0.71 -0.5
2,191,891 + +0.5
2,191,892 - +1.1
2,191,913 - -2.0
2,192,132 - ECD_02130 0.47 -1.4
2,192,190 + ECD_02130 0.60 -1.8
2,192,395 - +0.6
2,192,403 + -0.3
2,192,403 + -1.4
2,192,404 - +0.6
2,192,404 - -0.1
2,192,408 - -0.5
2,192,408 - +0.5
2,192,408 - +0.6
2,192,408 - +0.7
2,192,504 - ECD_02131 0.16 +0.8
2,192,504 - ECD_02131 0.16 +1.2
2,192,610 - ECD_02131 0.28 +0.1
2,192,663 - ECD_02131 0.34 +0.0
2,192,689 - ECD_02131 0.38 -1.2
2,192,777 - ECD_02131 0.48 -0.8
2,192,879 - ECD_02131 0.59 -1.7
2,193,069 - ECD_02131 0.81 +1.9
2,193,266 + -0.4
2,193,266 + -1.5
2,193,267 - +0.6
2,193,340 + ECD_02132 0.18 -2.0
2,193,693 + ECD_02132 0.69 -0.5
2,194,033 - +0.7
2,194,033 - -0.4
2,194,033 - +1.6

Or see this region's nucleotide sequence