Strain Fitness in Escherichia coli BL21 around ECD_01822

Experiment: EV116

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01820 and ECD_01821 are separated by 55 nucleotidesECD_01821 and ECD_01822 are separated by 36 nucleotidesECD_01822 and ECD_01823 are separated by 234 nucleotides ECD_01820: ECD_01820 - phosphoribosylglycinamide formyltransferase 2, at 1,876,605 to 1,877,783 _01820 ECD_01821: ECD_01821 - KHG/KDPG aldolase; 2-dehydro-3-deoxy-phosphogluconate/4-hydroxy-2- oxoglutarate aldolase, at 1,877,839 to 1,878,480 _01821 ECD_01822: ECD_01822 - 6-phosphogluconate dehydratase, at 1,878,517 to 1,880,328 _01822 ECD_01823: ECD_01823 - glucose-6-phosphate 1-dehydrogenase, at 1,880,563 to 1,882,038 _01823 Position (kb) 1878 1879 1880 1881Strain fitness (log2 ratio) -1 0 1at 1877.858 kb on - strandat 1877.992 kb on - strand, within ECD_01821at 1878.149 kb on - strand, within ECD_01821at 1878.741 kb on - strand, within ECD_01822at 1878.763 kb on - strand, within ECD_01822at 1878.865 kb on - strand, within ECD_01822at 1878.958 kb on - strand, within ECD_01822at 1879.165 kb on - strand, within ECD_01822at 1879.339 kb on + strand, within ECD_01822at 1879.659 kb on - strand, within ECD_01822at 1879.686 kb on - strand, within ECD_01822at 1879.686 kb on - strand, within ECD_01822at 1879.949 kb on - strand, within ECD_01822at 1880.262 kb on - strandat 1880.262 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV116
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1,877,858 - -1.3
1,877,992 - ECD_01821 0.24 +0.5
1,878,149 - ECD_01821 0.48 -0.9
1,878,741 - ECD_01822 0.12 -0.4
1,878,763 - ECD_01822 0.14 -1.0
1,878,865 - ECD_01822 0.19 +0.0
1,878,958 - ECD_01822 0.24 -0.2
1,879,165 - ECD_01822 0.36 +0.5
1,879,339 + ECD_01822 0.45 +1.7
1,879,659 - ECD_01822 0.63 +0.0
1,879,686 - ECD_01822 0.65 +0.7
1,879,686 - ECD_01822 0.65 +1.3
1,879,949 - ECD_01822 0.79 +0.9
1,880,262 - -0.9
1,880,262 - +1.0

Or see this region's nucleotide sequence