Strain Fitness in Escherichia coli BL21 around ECD_01820

Experiment: EV116

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01818 and ECD_01819 are separated by 66 nucleotidesECD_01819 and ECD_01820 are separated by 133 nucleotidesECD_01820 and ECD_01821 are separated by 55 nucleotidesECD_01821 and ECD_01822 are separated by 36 nucleotides ECD_01818: ECD_01818 - extracellular Colicin M immunity family protein, at 1,875,758 to 1,876,114 _01818 ECD_01819: ECD_01819 - DNA damage-inducible protein regulated by LexA, at 1,876,181 to 1,876,471 _01819 ECD_01820: ECD_01820 - phosphoribosylglycinamide formyltransferase 2, at 1,876,605 to 1,877,783 _01820 ECD_01821: ECD_01821 - KHG/KDPG aldolase; 2-dehydro-3-deoxy-phosphogluconate/4-hydroxy-2- oxoglutarate aldolase, at 1,877,839 to 1,878,480 _01821 ECD_01822: ECD_01822 - 6-phosphogluconate dehydratase, at 1,878,517 to 1,880,328 _01822 Position (kb) 1876 1877 1878Strain fitness (log2 ratio) -1 0 1at 1875.830 kb on - strand, within ECD_01818at 1876.449 kb on - strandat 1876.453 kb on - strandat 1876.654 kb on + strandat 1876.655 kb on - strandat 1876.657 kb on + strandat 1876.784 kb on + strand, within ECD_01820at 1876.838 kb on + strand, within ECD_01820at 1876.838 kb on + strand, within ECD_01820at 1876.860 kb on - strand, within ECD_01820at 1877.515 kb on + strand, within ECD_01820at 1877.858 kb on - strandat 1877.992 kb on - strand, within ECD_01821at 1878.149 kb on - strand, within ECD_01821at 1878.741 kb on - strand, within ECD_01822at 1878.763 kb on - strand, within ECD_01822

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Per-strain Table

Position Strand Gene LocusTag Fraction EV116
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1,875,830 - ECD_01818 0.20 +0.6
1,876,449 - -0.4
1,876,453 - +0.4
1,876,654 + -1.2
1,876,655 - +0.2
1,876,657 + -1.3
1,876,784 + ECD_01820 0.15 +0.7
1,876,838 + ECD_01820 0.20 +0.7
1,876,838 + ECD_01820 0.20 +0.7
1,876,860 - ECD_01820 0.22 -1.5
1,877,515 + ECD_01820 0.77 +1.2
1,877,858 - -1.3
1,877,992 - ECD_01821 0.24 +0.5
1,878,149 - ECD_01821 0.48 -0.9
1,878,741 - ECD_01822 0.12 -0.4
1,878,763 - ECD_01822 0.14 -1.0

Or see this region's nucleotide sequence