Strain Fitness in Escherichia coli BL21 around ECD_01278

Experiment: EV116

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01277 and ECD_01278 are separated by 1 nucleotidesECD_01278 and ECD_01279 are separated by 37 nucleotides ECD_01277: ECD_01277 - gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase; succinate semialdehyde dehydrogenase, at 1,349,109 to 1,350,596 _01277 ECD_01278: ECD_01278 - gamma-glutamylputrescine oxidoreductase, at 1,350,598 to 1,351,878 _01278 ECD_01279: ECD_01279 - 4-aminobutyrate aminotransferase, PLP-dependent, at 1,351,916 to 1,353,181 _01279 Position (kb) 1350 1351 1352Strain fitness (log2 ratio) -1 0 1 2at 1350.073 kb on + strand, within ECD_01277at 1350.170 kb on + strand, within ECD_01277at 1350.287 kb on - strand, within ECD_01277at 1350.505 kb on - strandat 1350.619 kb on + strandat 1350.622 kb on + strandat 1350.826 kb on + strand, within ECD_01278at 1351.102 kb on + strand, within ECD_01278at 1351.201 kb on + strand, within ECD_01278at 1351.202 kb on - strand, within ECD_01278at 1351.508 kb on - strand, within ECD_01278at 1351.508 kb on - strand, within ECD_01278at 1351.635 kb on + strand, within ECD_01278at 1351.672 kb on + strand, within ECD_01278at 1351.847 kb on - strandat 1352.039 kb on + strandat 1352.340 kb on - strand, within ECD_01279at 1352.418 kb on + strand, within ECD_01279at 1352.419 kb on - strand, within ECD_01279at 1352.521 kb on + strand, within ECD_01279at 1352.611 kb on + strand, within ECD_01279

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Per-strain Table

Position Strand Gene LocusTag Fraction EV116
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1,350,073 + ECD_01277 0.65 -0.7
1,350,170 + ECD_01277 0.71 +0.4
1,350,287 - ECD_01277 0.79 +1.2
1,350,505 - +1.2
1,350,619 + +0.8
1,350,622 + -0.7
1,350,826 + ECD_01278 0.18 +2.5
1,351,102 + ECD_01278 0.39 +0.6
1,351,201 + ECD_01278 0.47 +0.6
1,351,202 - ECD_01278 0.47 +0.1
1,351,508 - ECD_01278 0.71 -0.7
1,351,508 - ECD_01278 0.71 +1.0
1,351,635 + ECD_01278 0.81 +0.0
1,351,672 + ECD_01278 0.84 +0.2
1,351,847 - +0.1
1,352,039 + +0.8
1,352,340 - ECD_01279 0.33 +0.0
1,352,418 + ECD_01279 0.40 +0.4
1,352,419 - ECD_01279 0.40 +0.0
1,352,521 + ECD_01279 0.48 -0.0
1,352,611 + ECD_01279 0.55 -0.5

Or see this region's nucleotide sequence