Strain Fitness in Escherichia coli BL21 around ECD_01181

Experiment: EV116

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01179 and ECD_01180 are separated by 277 nucleotidesECD_01180 and ECD_01181 are separated by 54 nucleotidesECD_01181 and ECD_01182 are separated by 124 nucleotides ECD_01179: ECD_01179 - peptidyl-tRNA hydrolase, at 1,246,077 to 1,246,661 _01179 ECD_01180: ECD_01180 - DUF2583 family putative inner membrane protein, at 1,246,939 to 1,247,217 _01180 ECD_01181: ECD_01181 - C4-dicarboxylic acid transporter, at 1,247,272 to 1,248,951 _01181 ECD_01182: ECD_01182 - phosphoribosylpyrophosphate synthase, at 1,249,076 to 1,250,023 _01182 Position (kb) 1247 1248 1249Strain fitness (log2 ratio) -1 0 1at 1246.755 kb on - strandat 1246.802 kb on + strandat 1246.855 kb on + strandat 1246.876 kb on + strandat 1246.887 kb on + strandat 1246.938 kb on + strandat 1247.046 kb on + strand, within ECD_01180at 1247.211 kb on + strandat 1247.274 kb on - strandat 1247.594 kb on - strand, within ECD_01181at 1247.619 kb on + strand, within ECD_01181at 1247.620 kb on - strand, within ECD_01181at 1248.045 kb on + strand, within ECD_01181at 1248.370 kb on - strand, within ECD_01181at 1248.453 kb on - strand, within ECD_01181at 1248.564 kb on - strand, within ECD_01181at 1248.765 kb on - strand, within ECD_01181at 1248.828 kb on + strandat 1249.010 kb on + strandat 1249.018 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV116
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1,246,755 - -0.7
1,246,802 + +1.7
1,246,855 + -0.1
1,246,876 + -0.1
1,246,887 + -1.0
1,246,938 + -0.0
1,247,046 + ECD_01180 0.38 -0.3
1,247,211 + -0.3
1,247,274 - -1.2
1,247,594 - ECD_01181 0.19 -0.2
1,247,619 + ECD_01181 0.21 +1.7
1,247,620 - ECD_01181 0.21 -0.4
1,248,045 + ECD_01181 0.46 -0.1
1,248,370 - ECD_01181 0.65 +0.4
1,248,453 - ECD_01181 0.70 +0.0
1,248,564 - ECD_01181 0.77 +0.9
1,248,765 - ECD_01181 0.89 -0.3
1,248,828 + +0.6
1,249,010 + -0.2
1,249,018 - -0.5

Or see this region's nucleotide sequence