Strain Fitness in Escherichia coli BL21 around ECD_00015

Experiment: EV116

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00014 and ECD_00015 are separated by 88 nucleotidesECD_00015 and ECD_00016 are separated by 146 nucleotides ECD_00014: ECD_00014 - chaperone Hsp70, with co-chaperone DnaJ, at 12,161 to 14,077 _00014 ECD_00015: ECD_00015 - chaperone Hsp40, DnaK co-chaperone, at 14,166 to 15,296 _00015 ECD_00016: ECD_00016 - IS186 hypothetical protein, at 15,443 to 16,555 _00016 Position (kb) 14 15 16Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 13.246 kb on + strand, within ECD_00014at 13.272 kb on + strand, within ECD_00014at 13.326 kb on + strand, within ECD_00014at 13.326 kb on + strand, within ECD_00014at 13.758 kb on + strand, within ECD_00014at 14.084 kb on + strandat 14.084 kb on + strandat 14.086 kb on + strandat 14.086 kb on + strandat 14.098 kb on + strandat 14.098 kb on + strandat 14.098 kb on + strandat 14.098 kb on + strandat 14.098 kb on + strandat 14.100 kb on + strandat 14.100 kb on + strandat 14.171 kb on + strandat 14.184 kb on + strandat 14.259 kb on + strandat 14.306 kb on + strand, within ECD_00015at 14.325 kb on + strand, within ECD_00015at 14.325 kb on + strand, within ECD_00015at 14.325 kb on + strand, within ECD_00015at 14.361 kb on + strand, within ECD_00015at 14.362 kb on - strand, within ECD_00015at 14.370 kb on + strand, within ECD_00015at 14.399 kb on + strand, within ECD_00015at 14.520 kb on + strand, within ECD_00015at 14.956 kb on - strand, within ECD_00015at 15.337 kb on + strandat 15.354 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV116
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13,246 + ECD_00014 0.57 +3.1
13,272 + ECD_00014 0.58 +1.6
13,326 + ECD_00014 0.61 +3.1
13,326 + ECD_00014 0.61 +3.1
13,758 + ECD_00014 0.83 +1.2
14,084 + +0.6
14,084 + -3.7
14,086 + -1.0
14,086 + -0.6
14,098 + -1.2
14,098 + -1.1
14,098 + -0.6
14,098 + +0.2
14,098 + +0.0
14,100 + -4.1
14,100 + -0.9
14,171 + -1.5
14,184 + -2.5
14,259 + -2.4
14,306 + ECD_00015 0.12 -2.0
14,325 + ECD_00015 0.14 -2.9
14,325 + ECD_00015 0.14 -1.2
14,325 + ECD_00015 0.14 -3.0
14,361 + ECD_00015 0.17 -2.3
14,362 - ECD_00015 0.17 -1.1
14,370 + ECD_00015 0.18 +0.2
14,399 + ECD_00015 0.21 -3.3
14,520 + ECD_00015 0.31 -0.2
14,956 - ECD_00015 0.70 -2.3
15,337 + +0.6
15,354 - +1.0

Or see this region's nucleotide sequence