Strain Fitness in Escherichia coli BL21 around ECD_01259

Experiment: EV116

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01257 and ECD_01258 are separated by 192 nucleotidesECD_01258 and ECD_01259 overlap by 1 nucleotidesECD_01259 and ECD_01260 are separated by 125 nucleotidesECD_01260 and ECD_01261 are separated by 268 nucleotides ECD_01257: ECD_01257 - LPS regulatory protein; putative modulator of LpxC proteolysis, at 1,326,928 to 1,328,097 _01257 ECD_01258: ECD_01258 - orotidine-5'-phosphate decarboxylase, at 1,328,290 to 1,329,027 _01258 ECD_01259: ECD_01259 - initiation factor function partial mimic, SUI1 family, at 1,329,027 to 1,329,353 _01259 ECD_01260: ECD_01260 - osmotically and stress inducible lipoprotein, at 1,329,479 to 1,329,697 _01260 ECD_01261: ECD_01261 - global regulator of transcription; DeoR family, at 1,329,966 to 1,330,715 _01261 Position (kb) 1329 1330Strain fitness (log2 ratio) -1 0 1 2 3at 1328.361 kb on + strandat 1328.439 kb on + strand, within ECD_01258at 1328.439 kb on + strand, within ECD_01258at 1328.940 kb on + strand, within ECD_01258at 1330.082 kb on - strand, within ECD_01261at 1330.167 kb on - strand, within ECD_01261at 1330.281 kb on - strand, within ECD_01261

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Per-strain Table

Position Strand Gene LocusTag Fraction EV116
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1,328,361 + +3.7
1,328,439 + ECD_01258 0.20 -0.8
1,328,439 + ECD_01258 0.20 -1.1
1,328,940 + ECD_01258 0.88 -0.4
1,330,082 - ECD_01261 0.15 -0.3
1,330,167 - ECD_01261 0.27 +0.4
1,330,281 - ECD_01261 0.42 -1.3

Or see this region's nucleotide sequence