Strain Fitness in Escherichia coli BL21 around ECD_01012

Experiment: EV116

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01010 and ECD_01011 are separated by 10 nucleotidesECD_01011 and ECD_01012 are separated by 9 nucleotidesECD_01012 and ECD_01013 are separated by 7 nucleotidesECD_01013 and ECD_01014 are separated by 11 nucleotides ECD_01010: ECD_01010 - flavin:NADH reductase, at 1,074,142 to 1,074,636 _01010 ECD_01011: ECD_01011 - putative malonic semialdehyde reductase, at 1,074,647 to 1,075,237 _01011 ECD_01012: ECD_01012 - putative reactive intermediate detoxifying aminoacrylate hydrolase, at 1,075,247 to 1,076,047 _01012 ECD_01013: ECD_01013 - putative aminoacrylate deaminase, reactive intermediate detoxification; weak enamine/imine deaminase activity, at 1,076,055 to 1,076,441 _01013 ECD_01014: ECD_01014 - ureidoacrylate amidohydrolase, at 1,076,453 to 1,077,145 _01014 Position (kb) 1075 1076 1077Strain fitness (log2 ratio) -2 -1 0 1 2at 1074.649 kb on - strandat 1074.727 kb on + strand, within ECD_01011at 1075.129 kb on - strand, within ECD_01011at 1075.271 kb on + strandat 1075.272 kb on - strandat 1075.477 kb on + strand, within ECD_01012at 1075.501 kb on + strand, within ECD_01012at 1075.867 kb on - strand, within ECD_01012at 1075.892 kb on - strand, within ECD_01012at 1076.204 kb on + strand, within ECD_01013at 1076.324 kb on + strand, within ECD_01013at 1076.325 kb on - strand, within ECD_01013at 1076.356 kb on + strand, within ECD_01013at 1076.356 kb on + strand, within ECD_01013at 1076.356 kb on + strand, within ECD_01013at 1076.357 kb on - strand, within ECD_01013at 1076.357 kb on - strand, within ECD_01013at 1076.848 kb on + strand, within ECD_01014at 1076.913 kb on + strand, within ECD_01014at 1076.914 kb on - strand, within ECD_01014at 1077.038 kb on + strand, within ECD_01014

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Per-strain Table

Position Strand Gene LocusTag Fraction EV116
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1,074,649 - -1.0
1,074,727 + ECD_01011 0.14 +0.0
1,075,129 - ECD_01011 0.82 +2.2
1,075,271 + +1.4
1,075,272 - +1.0
1,075,477 + ECD_01012 0.29 -0.8
1,075,501 + ECD_01012 0.32 -0.8
1,075,867 - ECD_01012 0.77 -0.3
1,075,892 - ECD_01012 0.81 +0.5
1,076,204 + ECD_01013 0.39 -1.1
1,076,324 + ECD_01013 0.70 +0.2
1,076,325 - ECD_01013 0.70 -0.4
1,076,356 + ECD_01013 0.78 +0.3
1,076,356 + ECD_01013 0.78 -0.4
1,076,356 + ECD_01013 0.78 +0.2
1,076,357 - ECD_01013 0.78 -0.4
1,076,357 - ECD_01013 0.78 -1.8
1,076,848 + ECD_01014 0.57 +0.4
1,076,913 + ECD_01014 0.66 +0.3
1,076,914 - ECD_01014 0.67 +0.2
1,077,038 + ECD_01014 0.84 -2.2

Or see this region's nucleotide sequence