Strain Fitness in Escherichia coli BL21 around ECD_00354

Experiment: EV116

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00353 and ECD_00354 are separated by 55 nucleotidesECD_00354 and ECD_00355 are separated by 22 nucleotidesECD_00355 and ECD_00356 are separated by 27 nucleotides ECD_00353: ECD_00353 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 390,413 to 391,483 _00353 ECD_00354: ECD_00354 - tRNA-guanine transglycosylase, at 391,539 to 392,666 _00354 ECD_00355: ECD_00355 - SecYEG protein translocase auxillary subunit, at 392,689 to 393,021 _00355 ECD_00356: ECD_00356 - SecYEG protein translocase auxillary subunit, at 393,049 to 394,896 _00356 Position (kb) 391 392 393Strain fitness (log2 ratio) -3 -2 -1 0 1at 390.866 kb on + strand, within ECD_00353at 390.866 kb on + strand, within ECD_00353at 391.186 kb on - strand, within ECD_00353at 391.261 kb on + strand, within ECD_00353at 391.337 kb on - strand, within ECD_00353at 391.438 kb on + strandat 391.445 kb on + strandat 391.445 kb on + strandat 391.462 kb on + strandat 391.526 kb on + strandat 391.526 kb on + strandat 391.844 kb on + strand, within ECD_00354at 391.974 kb on - strand, within ECD_00354at 392.119 kb on + strand, within ECD_00354at 392.207 kb on + strand, within ECD_00354at 392.230 kb on + strand, within ECD_00354at 392.230 kb on + strand, within ECD_00354at 392.230 kb on + strand, within ECD_00354at 392.409 kb on + strand, within ECD_00354at 392.411 kb on + strand, within ECD_00354at 392.411 kb on + strand, within ECD_00354at 392.451 kb on + strand, within ECD_00354at 392.451 kb on + strand, within ECD_00354at 392.478 kb on + strand, within ECD_00354at 392.478 kb on + strand, within ECD_00354at 392.550 kb on + strand, within ECD_00354at 392.644 kb on + strandat 392.644 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV116
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390,866 + ECD_00353 0.42 +0.3
390,866 + ECD_00353 0.42 -2.0
391,186 - ECD_00353 0.72 -0.6
391,261 + ECD_00353 0.79 -0.1
391,337 - ECD_00353 0.86 -2.1
391,438 + +1.6
391,445 + +0.6
391,445 + +0.5
391,462 + -1.0
391,526 + -0.2
391,526 + +0.2
391,844 + ECD_00354 0.27 -1.2
391,974 - ECD_00354 0.39 +0.2
392,119 + ECD_00354 0.51 -2.5
392,207 + ECD_00354 0.59 +0.4
392,230 + ECD_00354 0.61 -0.9
392,230 + ECD_00354 0.61 -1.8
392,230 + ECD_00354 0.61 -0.2
392,409 + ECD_00354 0.77 +0.6
392,411 + ECD_00354 0.77 -0.9
392,411 + ECD_00354 0.77 -3.0
392,451 + ECD_00354 0.81 -0.7
392,451 + ECD_00354 0.81 -0.3
392,478 + ECD_00354 0.83 -1.6
392,478 + ECD_00354 0.83 +0.2
392,550 + ECD_00354 0.90 +0.2
392,644 + -3.3
392,644 + +0.3

Or see this region's nucleotide sequence