Strain Fitness in Escherichia coli BL21 around ECD_00327

Experiment: EV116

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00325 and ECD_00326 are separated by 58 nucleotidesECD_00326 and ECD_00327 are separated by 259 nucleotidesECD_00327 and ECD_00328 are separated by 23 nucleotides ECD_00325: ECD_00325 - microcin Bac7 uptake protein; outer membrane surface-exposed lipoprotein, at 362,497 to 363,591 _00325 ECD_00326: ECD_00326 - DUF2755 family inner membrane protein, at 363,650 to 363,958 _00326 ECD_00327: ECD_00327 - DUF2754 family putative inner membrane protein, at 364,218 to 364,430 _00327 ECD_00328: ECD_00328 - D-alanine-D-alanine ligase A, at 364,454 to 365,548 _00328 Position (kb) 364 365Strain fitness (log2 ratio) -2 -1 0 1 2 3at 363.304 kb on - strand, within ECD_00325at 363.387 kb on - strand, within ECD_00325at 363.579 kb on + strandat 363.580 kb on - strandat 363.625 kb on + strandat 363.645 kb on - strandat 363.645 kb on - strandat 363.645 kb on - strandat 363.713 kb on + strand, within ECD_00326at 364.157 kb on + strandat 364.254 kb on + strand, within ECD_00327at 364.254 kb on + strand, within ECD_00327at 364.257 kb on + strand, within ECD_00327at 364.277 kb on + strand, within ECD_00327at 364.280 kb on - strand, within ECD_00327at 364.339 kb on - strand, within ECD_00327at 364.367 kb on + strand, within ECD_00327at 364.434 kb on + strandat 364.570 kb on + strand, within ECD_00328at 364.570 kb on + strand, within ECD_00328at 364.571 kb on - strand, within ECD_00328at 364.571 kb on - strand, within ECD_00328at 364.571 kb on - strand, within ECD_00328at 364.571 kb on - strand, within ECD_00328at 364.770 kb on + strand, within ECD_00328at 364.770 kb on + strand, within ECD_00328at 364.770 kb on + strand, within ECD_00328at 364.771 kb on - strand, within ECD_00328at 364.789 kb on - strand, within ECD_00328at 364.901 kb on - strand, within ECD_00328at 364.956 kb on - strand, within ECD_00328at 364.956 kb on - strand, within ECD_00328at 364.998 kb on - strand, within ECD_00328at 365.166 kb on - strand, within ECD_00328at 365.174 kb on + strand, within ECD_00328at 365.417 kb on - strand, within ECD_00328

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Per-strain Table

Position Strand Gene LocusTag Fraction EV116
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363,304 - ECD_00325 0.74 +1.4
363,387 - ECD_00325 0.81 +1.5
363,579 + -0.7
363,580 - -0.3
363,625 + +0.4
363,645 - -0.9
363,645 - +0.6
363,645 - +0.1
363,713 + ECD_00326 0.20 +0.4
364,157 + -1.5
364,254 + ECD_00327 0.17 -0.4
364,254 + ECD_00327 0.17 +0.1
364,257 + ECD_00327 0.18 -0.4
364,277 + ECD_00327 0.28 -0.3
364,280 - ECD_00327 0.29 +1.7
364,339 - ECD_00327 0.57 +3.2
364,367 + ECD_00327 0.70 +0.1
364,434 + -1.8
364,570 + ECD_00328 0.11 -0.4
364,570 + ECD_00328 0.11 -1.0
364,571 - ECD_00328 0.11 +0.7
364,571 - ECD_00328 0.11 -0.4
364,571 - ECD_00328 0.11 +0.2
364,571 - ECD_00328 0.11 +0.9
364,770 + ECD_00328 0.29 -0.0
364,770 + ECD_00328 0.29 -0.3
364,770 + ECD_00328 0.29 +1.3
364,771 - ECD_00328 0.29 -0.1
364,789 - ECD_00328 0.31 -0.5
364,901 - ECD_00328 0.41 -0.1
364,956 - ECD_00328 0.46 +0.2
364,956 - ECD_00328 0.46 -1.5
364,998 - ECD_00328 0.50 -0.2
365,166 - ECD_00328 0.65 +1.1
365,174 + ECD_00328 0.66 -1.7
365,417 - ECD_00328 0.88 +1.1

Or see this region's nucleotide sequence