Strain Fitness in Escherichia coli BL21 around ECD_r00005

Experiment: Bas18

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02481 and ECD_r00004 are separated by 320 nucleotidesECD_r00004 and ECD_r00005 are separated by 92 nucleotidesECD_r00005 and ECD_t00045 are separated by 184 nucleotidesECD_t00045 and ECD_r00006 are separated by 85 nucleotides ECD_02481: ECD_02481 - alpha-ketoglutarate transporter, at 2,591,048 to 2,592,346 _02481 ECD_r00004: ECD_r00004 - 5S ribosomal RNA, at 2,592,667 to 2,592,786 _r00004 ECD_r00005: ECD_r00005 - 23S ribosomal RNA, at 2,592,879 to 2,595,784 _r00005 ECD_t00045: ECD_t00045 - tRNA-Glu, at 2,595,969 to 2,596,044 _t00045 ECD_r00006: ECD_r00006 - 16S ribosomal RNA, at 2,596,130 to 2,597,671 _r00006 Position (kb) 2592 2593 2594 2595 2596Strain fitness (log2 ratio) -1 0 1at 2591.902 kb on + strand, within ECD_02481at 2591.904 kb on + strand, within ECD_02481at 2591.904 kb on + strand, within ECD_02481at 2591.905 kb on - strand, within ECD_02481at 2591.905 kb on - strand, within ECD_02481at 2591.905 kb on - strand, within ECD_02481at 2591.969 kb on + strand, within ECD_02481at 2592.044 kb on - strand, within ECD_02481at 2592.162 kb on - strand, within ECD_02481at 2592.574 kb on + strandat 2592.574 kb on + strandat 2592.574 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas18
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2,591,902 + ECD_02481 0.66 +0.3
2,591,904 + ECD_02481 0.66 +0.7
2,591,904 + ECD_02481 0.66 -0.9
2,591,905 - ECD_02481 0.66 -0.1
2,591,905 - ECD_02481 0.66 +0.1
2,591,905 - ECD_02481 0.66 -0.6
2,591,969 + ECD_02481 0.71 +0.6
2,592,044 - ECD_02481 0.77 +0.3
2,592,162 - ECD_02481 0.86 +0.3
2,592,574 + -0.9
2,592,574 + -1.1
2,592,574 + -1.1

Or see this region's nucleotide sequence