Experiment: Bas18
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_02481 and ECD_r00004 are separated by 320 nucleotides ECD_r00004 and ECD_r00005 are separated by 92 nucleotides ECD_r00005 and ECD_t00045 are separated by 184 nucleotides ECD_t00045 and ECD_r00006 are separated by 85 nucleotides
ECD_02481: ECD_02481 - alpha-ketoglutarate transporter, at 2,591,048 to 2,592,346
_02481
ECD_r00004: ECD_r00004 - 5S ribosomal RNA, at 2,592,667 to 2,592,786
_r00004
ECD_r00005: ECD_r00005 - 23S ribosomal RNA, at 2,592,879 to 2,595,784
_r00005
ECD_t00045: ECD_t00045 - tRNA-Glu, at 2,595,969 to 2,596,044
_t00045
ECD_r00006: ECD_r00006 - 16S ribosomal RNA, at 2,596,130 to 2,597,671
_r00006
Position (kb)
2592
2593
2594
2595
2596 Strain fitness (log2 ratio)
-1
0
1 at 2591.902 kb on + strand, within ECD_02481 at 2591.904 kb on + strand, within ECD_02481 at 2591.904 kb on + strand, within ECD_02481 at 2591.905 kb on - strand, within ECD_02481 at 2591.905 kb on - strand, within ECD_02481 at 2591.905 kb on - strand, within ECD_02481 at 2591.969 kb on + strand, within ECD_02481 at 2592.044 kb on - strand, within ECD_02481 at 2592.162 kb on - strand, within ECD_02481 at 2592.574 kb on + strand at 2592.574 kb on + strand at 2592.574 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas18 remove 2,591,902 + ECD_02481 0.66 +0.3 2,591,904 + ECD_02481 0.66 +0.7 2,591,904 + ECD_02481 0.66 -0.9 2,591,905 - ECD_02481 0.66 -0.1 2,591,905 - ECD_02481 0.66 +0.1 2,591,905 - ECD_02481 0.66 -0.6 2,591,969 + ECD_02481 0.71 +0.6 2,592,044 - ECD_02481 0.77 +0.3 2,592,162 - ECD_02481 0.86 +0.3 2,592,574 + -0.9 2,592,574 + -1.1 2,592,574 + -1.1
Or see this region's nucleotide sequence