Strain Fitness in Escherichia coli BL21 around ECD_01694

Experiment: Bas18

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01692 and ECD_01693 are separated by 100 nucleotidesECD_01693 and ECD_01694 are separated by 105 nucleotidesECD_01694 and ECD_01695 are separated by 40 nucleotidesECD_01695 and ECD_01696 are separated by 146 nucleotides ECD_01692: ECD_01692 - 6-phosphofructokinase II, at 1,750,783 to 1,751,712 _01692 ECD_01693: ECD_01693 - uncharacterized protein, at 1,751,813 to 1,752,103 _01693 ECD_01694: ECD_01694 - fructosamine kinase family protein, at 1,752,209 to 1,753,069 _01694 ECD_01695: ECD_01695 - putative inner membrane protein, at 1,753,110 to 1,753,646 _01695 ECD_01696: ECD_01696 - 2-deoxyglucose-6-P phosphatase, at 1,753,793 to 1,754,461 _01696 Position (kb) 1752 1753 1754Strain fitness (log2 ratio) -2 -1 0 1at 1751.319 kb on + strand, within ECD_01692at 1751.496 kb on + strand, within ECD_01692at 1751.838 kb on - strandat 1751.867 kb on - strand, within ECD_01693at 1751.893 kb on + strand, within ECD_01693at 1751.926 kb on + strand, within ECD_01693at 1752.413 kb on - strand, within ECD_01694at 1752.943 kb on - strand, within ECD_01694at 1753.175 kb on - strand, within ECD_01695at 1753.691 kb on + strandat 1753.691 kb on + strandat 1753.739 kb on - strandat 1753.744 kb on + strandat 1753.759 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas18
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1,751,319 + ECD_01692 0.58 +1.0
1,751,496 + ECD_01692 0.77 +0.4
1,751,838 - +0.3
1,751,867 - ECD_01693 0.19 -0.4
1,751,893 + ECD_01693 0.27 +0.0
1,751,926 + ECD_01693 0.39 +0.5
1,752,413 - ECD_01694 0.24 -0.5
1,752,943 - ECD_01694 0.85 -0.3
1,753,175 - ECD_01695 0.12 -0.5
1,753,691 + +0.3
1,753,691 + +0.7
1,753,739 - +0.6
1,753,744 + -2.1
1,753,759 - +1.1

Or see this region's nucleotide sequence